Genetic Transformation Systems in Fungi, Volume 1

Berg, M. A.; Maruthachalam, Karunakaran

doi:10.1007/978-3-319-10142-2

Fungal Biology

2015

DOI: 10.1007/978-3-319-10142-2

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Genetic Transformation Systems in Fungi, Volume 1

M. A. Berg¹,

Karunakaran Maruthachalam²

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Cited by 4 publications

References 323 publications

(538 reference statements)

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Genomic Variations and Mutational Events Associated with Plant–Pathogen Interactions

Dolatabadian

Fernando

2022

Biology

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Phytopathologists are actively researching the molecular basis of plant–pathogen interactions. The mechanisms of responses to pathogens have been studied extensively in model crop plant species and natural populations. Today, with the rapid expansion of genomic technologies such as DNA sequencing, transcriptomics, proteomics, and metabolomics, as well as the development of new methods and protocols, data analysis, and bioinformatics, it is now possible to assess the role of genetic variation in plant–microbe interactions and to understand the underlying molecular mechanisms of plant defense and microbe pathogenicity with ever-greater resolution and accuracy. Genetic variation is an important force in evolution that enables organisms to survive in stressful environments. Moreover, understanding the role of genetic variation and mutational events is essential for crop breeders to produce improved cultivars. This review focuses on genetic variations and mutational events associated with plant–pathogen interactions and discusses how these genome compartments enhance plants’ and pathogens’ evolutionary processes.

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Genomic Variations and Mutational Events Associated with Plant–Pathogen Interactions

Dolatabadian

Fernando

2022

Biology

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Genetic Engineering in Bacteria, Fungi, and Oomycetes, Taking Advantage of CRISPR

Yang,

Condrich,

et al. 2024

DNA

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Genetic engineering has revolutionized our ability to modify microorganisms for various applications in agriculture, medicine, and industry. This review examines recent advances in genetic engineering techniques for bacteria, fungi, and oomycetes, with a focus on CRISPR-Cas systems. In bacteria, CRISPR-Cas9 has enabled precise genome editing, enhancing applications in antibiotic production and metabolic engineering. For fungi, despite challenges associated with their complex cell structures, CRISPR/Cas9 has advanced the production of enzymes and secondary metabolites. In oomycetes, significant plant pathogens, modified Agrobacterium-mediated transformation, and CRISPR/Cas12a have contributed to developing disease-resistant crops. This review provides a comparative analysis of genetic engineering efficiencies across these microorganisms and addresses ethical and regulatory considerations. Future research directions include refining genetic tools to improve efficiency and expand applicability in non-model organisms. This comprehensive overview highlights the transformative potential of genetic engineering in microbiology and its implications for addressing global challenges in agriculture, medicine, and biotechnology.

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Identification of Aspergillus niger Aquaporins Involved in Hydrogen Peroxide Signaling

Laothanachareon

Asin-Garcia

Volkers

et al. 2023

JoF

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Aspergillus niger is a robust microbial cell factory for organic acid production. However, the regulation of many industrially important pathways is still poorly understood. The regulation of the glucose oxidase (Gox) expression system, involved in the biosynthesis of gluconic acid, has recently been uncovered. The results of that study show hydrogen peroxide, a by-product of the extracellular conversion of glucose to gluconate, has a pivotal role as a signaling molecule in the induction of this system. In this study, the facilitated diffusion of hydrogen peroxide via aquaporin water channels (AQPs) was studied. AQPs are transmembrane proteins of the major intrinsic proteins (MIPs) superfamily. In addition to water and glycerol, they may also transport small solutes such as hydrogen peroxide. The genome sequence of A. niger N402 was screened for putative AQPs. Seven AQPs were found and could be classified into three main groups. One protein (AQPA) belonged to orthodox AQP, three (AQPB, AQPD, and AQPE) were grouped in aquaglyceroporins (AQGP), two (AQPC and AQPF) were in X-intrinsic proteins (XIPs), and the other (AQPG) could not be classified. Their ability to facilitate diffusion of hydrogen peroxide was identified using yeast phenotypic growth assays and by studying AQP gene knock-outs in A. niger. The X-intrinsic protein AQPF appears to play roles in facilitating hydrogen peroxide transport across the cellular membrane in both Saccharomyces cerevisiae and A. niger experiments.

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Genetic Transformation Systems in Fungi, Volume 1

Cited by 4 publications

References 323 publications

Genomic Variations and Mutational Events Associated with Plant–Pathogen Interactions

Genomic Variations and Mutational Events Associated with Plant–Pathogen Interactions

Genetic Engineering in Bacteria, Fungi, and Oomycetes, Taking Advantage of CRISPR

Identification of Aspergillus niger Aquaporins Involved in Hydrogen Peroxide Signaling

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