Genetic transformation with the gfp gene of Colletotrichum gloeosporioides isolates from coffee with blister spot

Armesto, Cecilia; Maia, Fernanda Gonçalves Martins; Abreu, Márvio Lobão Teixeira de; Figueira, Antonia dos Reis; Silva, Bruno Marques da; Monteiro, Fernando Pereira

doi:10.1590/s1517-83822012000300050

Cited by 11 publications

(5 citation statements)

References 3 publications

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“…Nevertheless, the expression of GFP allows detection under UV light in both in vitro and in vivo experiments (Armesto et al, 2012), and it can be used as reporter for gene expression and localization in cell development studies (Lorang et al, 2001).…”

Section: Discussionmentioning

confidence: 99%

Agrobacterium-mediated transformation of Ceratocystis albifundus

Sayari

Nest

Steenkamp

et al. 2019

Microbiological Research

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Section: Discussionmentioning

confidence: 99%

Agrobacterium-mediated transformation of Ceratocystis albifundus

Sayari

Nest

Steenkamp

et al. 2019

Microbiological Research

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“…Conidia of F . temperatum were transformed, since the insertion of the GFP was randomized in the genome of the fungus, the effect of decreasing the expression of the GFP may be due to the number of copies inserted in the genome and the place of insertion (Armesto et al., 2012; Chen et al., 2011). The transforming colonies were selected by PCR.…”

Section: Discussionmentioning

confidence: 99%

Fusarium temperatum shows a hemibiotrophic infection process and differential pathogenicity over different maize breeds from Mexico

Robles‐Barrios

Ramírez-Granillo

Medina-Canales

et al. 2021

Journal of Phytopathology

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The genus Fusarium contains several species that cause diseases, such as drowning and wilting, in cultivable plants. Fusarium temperatum has been included in the Giberella fujikuroi complex and has been reported as a devastating pathogen for maize in Asia, Europe and South America. In Mexico, maize is one of the most important crops, so we consider it of great importance to describe the infection process of F. temperatum on maize plantlets and the susceptibility of several breeds of maize. We use in vivo assays to determine the infectivity of 6 maize breeds from Mexico. The most resistant breed was Pepitilla with less than 10 percent of damage, while Harinoso de 8, Tuxpeño and Tepecintle showed moderate resistance, while Reventador and Zapata 2 showed the most extensive damage. Because of its susceptibility, Zapata 2 was used to follow the infection process of F. temperatum. To follow the fungus in the infected roots we use a transformed strain that expresses GFPs. We found that the fungus directly penetrates the root tissue without forming an appressorium. We were able to establish a hemibiotrophic infection pattern for F. temperatum (biotrophic stage: 5–10 dpi; transition stage: 15 dpi; necrotrophic stage: 20 dpi). Our results described that F. temperatum presents a hemibiotropic infection pattern and suggests the presence of maize breeds in Mexico that have natural resistance to F. temperatum.

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“…Transformation efficiency can vary depending on the technology used and the organism transformed. Armesto et al (2012) obtained only 21 transformants from C . gloeosporioides (1 × 10 7 protoplasts/ml) using PEG-mediated transformation, whereas Talhinhas et al (2008) obtained 45–156 transformants of C .…”

Section: Discussionmentioning

confidence: 99%