Genetic typing of Malassezia pachydermatis from different domestic animals

Castellá, G.; Hernández, J. J.; Cabañes, F. Javier

doi:10.1016/j.vetmic.2005.04.016

Cited by 34 publications

(32 citation statements)

References 29 publications

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“…Genotypes I and II were detected more frequently in isolates from animals with clinical signs; genotype III was detected mostly in healthy animals; and genotype IV was distributed equally among animals with or without clinical signs (Figure 1). In our investigation, the distribution profi le of the genotypes is similar to that described by Castellá et al (2005), although in their study genotype III was found only in healthy animals. This is probably because the isolates used by Castellá et al (2005) were from only 11 healthy or sick animals (5 dogs, 3 cats, 1 horse, 1 goat and 1 pig).…”

Section: Resultssupporting

confidence: 87%

“…In our investigation, the distribution profi le of the genotypes is similar to that described by Castellá et al (2005), although in their study genotype III was found only in healthy animals. This is probably because the isolates used by Castellá et al (2005) were from only 11 healthy or sick animals (5 dogs, 3 cats, 1 horse, 1 goat and 1 pig).…”

Section: Resultssupporting

confidence: 87%

“…The amplification products were analyzed by electrophoresis in a 2% agarose gel stained with ethidium bromide (10 μgml -1 ), for 3 h at 12 Vcm -1 and observed in an UV transilluminator with a 100 bp DNA Ladder marker. The samples were classified into four genetic patterns, type I, II, III and IV, as described by Castellá et al (2005).…”

Section: Rapd Techniquementioning

confidence: 99%

“…The genetic diversity of M. pachydermatis has been identified by several molecular techniques such as the Amplified Fragment Length Polymorphisms(AFLP), Random Amplified Polymorphic DNA (RAPD) (CASTELLÁ et al, 2005;THEELEN et al, 2001) and analysis of sequences of the Internal Transcribed Spacers1 (ITS 1) (MAKIMURA et al, 2000), which suggested that some of the genotypes are pathogenic (CASTELLÁ et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

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Genotipificação de Malassezia pachydermatis através da técnica de RAPD

Kagueyama

Moraes

Rosa

et al. 2016

SCA

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Malassezia pachydermatis (M. pachydermatis) is a fungus of importance in human and veterinary medicine. Although a part of the normal microbiota, it can sometimes be present in its pathogenic form, particularly causing otitis and dermatitis in animals. Among human beings, it mainly affects immuno compromised patients and newborns, causing simple pustulosis, seborrheic dermatitis, tinea versicolor or fungemia. This study aimed to analyze the genomic polymorphism in M. pachydermatis samples isolated from Canis familiaris (domestic dog), Felis catus (domestic cat), and Myrmecophaga tridactyla (giant anteater). Two hundred and fourteen samples were collected and cultured in Sabouraud agar with chloranphenicol (100mg L -1 ) and incubated at 37 °C for a period of 7 to 10 days. One hundred and sixty six samples that appeared morphologically comparable to yeast cultures were processed for DNA extraction and PCR was performed for a specific region in the Internal Transcribed Spacer (ITS) of M. pachydermatis. Among these, seven (4.21%) were negative and 159 (95.79%) were positive. Of the 159 positive samples, 102 (64.15%) were from animals with clinical signs and 57 (35.85%) without clinical signs. Fifty-seven samples were selected at random for RAPD-PCR based genotyping and distributed into four genetic groups. Types I and II were more frequent in animals with clinical signs while type III was frequent in healthy animals. Type IV occurred evenly across animals with or without clinical signs. These results indicate differences in pathogenicity of the fungus based on the genotype. Key words: Malassezia pachydermatis. PCR. RAPD. ResumoA levedura Malassezia pachydermatis é de importância na medicina humana e veterinária por se apresentar de forma comensal e por vezes sob a forma patogênica. Em animais, causa principalmente otites e dermatites e em humanos acomete principalmente pacientes imunocomprometidos e neonatos, causando desde pustulose simples, dermatite seborréica, pitiríase versicolor até fungemia.

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Section: Resultssupporting

confidence: 87%

Section: Resultssupporting

confidence: 87%

Section: Rapd Techniquementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Genotipificação de Malassezia pachydermatis através da técnica de RAPD

Kagueyama

Moraes

Rosa

et al. 2016

SCA

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“…In PCR-fingerprinting, the same approach as PCR-RAPD is employed, but the primer used is specific for mini-satellite DNA [29,30 ]. The drawback of these Molecular methods for typing Malassezia Gaitanis et al 123 Figure 1 The rRNA gene complex consists of multiple copies of the small subunit or 18S, the 5.8S region flanked by the internal transcribed spacer regions 1 and 2, the large subunit or 28S and the intergenic spacer regions 1 and 2 that flank the 5S rDNA IGS, intergenic spacer region; ITS, internal transcribed spacer; LSU, large subunit; SSU, small subunit.…”

Section: Polymorphic Dna Amplification Methodsmentioning

confidence: 99%

The range of molecular methods for typing Malassezia

Gaitanis

Bassukas

Velegraki

2009

Current Opinion in Infectious Diseases

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Current awareness on yeast

2006

Yeast

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In order to keep subscribers up‐to‐date with the latest developments in their field, this current awareness service is provided by John Wiley & Sons and contains newly‐published material on yeasts. Each bibliography is divided into 10 sections. 1 Books, Reviews & Symposia; 2 General; 3 Biochemistry; 4 Biotechnology; 5 Cell Biology; 6 Gene Expression; 7 Genetics; 8 Physiology; 9 Medical Mycology; 10 Recombinant DNA Technology. Within each section, articles are listed in alphabetical order with respect to author. If, in the preceding period, no publications are located relevant to any one of these headings, that section will be omitted. (4 weeks journals ‐ search completed 16th. Nov. 2004)

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Genetic typing of Malassezia pachydermatis from different domestic animals

Cited by 34 publications

References 29 publications

Genotipificação de Malassezia pachydermatis através da técnica de RAPD

Genotipificação de Malassezia pachydermatis através da técnica de RAPD

The range of molecular methods for typing Malassezia

Current awareness on yeast

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