Genetic Variability Analysis of Phaius spp Orchid based on RAPD Markers

Abstract

Phaius spp live in tropical forests with high humidity due to deforestation, which has resulted in the extinction of many endemic orchid species, especially orchids Phaius spp, so it needs to be conserved through a plant breeding program. There are not many research reports on the genetic diversity of orchids Phaius spp, even though the study of genetic diversity plays an important role in plant breeding programs and genetic conversation. This study aims to determine the genetic diversity of Phaius spp. This research was carried out using molecular markers RAPD (Random Amplified Polymorphic DNA). DNA from Phaius spp was extracted and then amplified by PCR using 6 RAPD primers. The results of this study found that four species of orchids of the genus Phaius spp (Phaius tankervillae (1); Phaius montanus (2); Phaius collasus (3); and Phaius amboinensis (4)) amplified using 6 RAPD primers to produce 37 DNA bands with a size of 250-1600 bp, and produce 100% polymorphic bands The genetic similarity coefficient of the 4 orchid species ranges from 0.19-0.53 and the genetic variability ranges from 47%-81%.

Genetic similarity among Dendrobium species from Indonesia using RAPD markers

“…In this study we used five RAPD primers from the 20 primers (Operon Technology Ltd) that were selected previously. The five selected primers, namely OPD-8 (Dendrobium (Choopeng et al 2019); OPA-7 (Dendrobium (Tikendra et al 2019a), Coelogyne (Hartati 2020), Phaius (Hartati et al 2021), Rhynchostylis retusa (Oliya et al 2021), Dendrobium (Joshi et al 2023)); OPB-12, OPA-13 and OPB-18 (Coelogyne (Hartati 2020), Phaius (Hartati and Samanhudi 2022) have been shown to produce polymorphic bands in orchids. PCR reaction was performed with a total volume of 15 µL containing 0.2 n MdNTP; 1 x reaction buffer; 2mM MgCl2; DNA sample 25 ng; 1 pmol; and 1 unit of Taq DNA polymerase (Promega) using a Thermocycler (Takara) for 45 cycles.…”

Genetic Diversity and Relationship among and within the Bird of Paradise Plants of Strelitziaceae and Heliconiaceae

“…RAPD is considered adequate genotype identification and analysis of genetic differences among induced mutants (Wahyudi et al 2020). In addition to Heliconiaceae and Streltiziceae, RAPD has been popularly applied at inter-and intra-specific levels of various plant species, such as wheat and barley (Ghabeish et al, 2021), rice (Sholikhah et al, 2019), ramie (Mayerni et al, 2019), legumes (Purwanti et al, 2021), pea (Osman and Ali, 2021), orchid (Hartati and Samanhudi, 2022), kalanchoe (Al-Khayri et al, 2022), banana (Probojati et al, 2019;Wiguna and Pharmawati, 2021), ginger (Trimanto et al 2023), and many more. RAPD is a DNA amplification technique based on PCR using random primers and generally consists of 10 arbitrary base sequences with a minimum content of G and C bases of 60%.…”

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“…RAPD is considered adequate genotype identification and analysis of genetic differences among induced mutants (Wahyudi et al 2020). In addition to Heliconiaceae and Streltiziceae, RAPD has been popularly applied at inter-and intra-specific levels of various plant species, such as wheat and barley (Ghabeish et al, 2021), rice (Sholikhah et al, 2019), ramie (Mayerni et al, 2019), legumes (Purwanti et al, 2021), pea (Osman and Ali, 2021), orchid (Hartati and Samanhudi, 2022), kalanchoe (Al-Khayri et al, 2022), banana (Probojati et al, 2019;Wiguna and Pharmawati, 2021), ginger (Trimanto et al 2023), and many more. RAPD is a DNA amplification technique based on PCR using random primers and generally consists of 10 arbitrary base sequences with a minimum content of G and C bases of 60%.…”