Genetic variability and association of AFLP markers with some important biochemical traits in Dendrobium thyrsiflorum, a threatened medicinal orchid

Bhattacharyya, Paromik; Ghosh, Sumon; Mandi, Swati Sen; Kumaria, Suman; Tandon, Pramod

doi:10.1016/j.sajb.2016.12.012

Cited by 23 publications

(8 citation statements)

References 72 publications

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“…In breeding projects, achieving favorable plant materials is mostly a time‐consuming operation, and consequently, marker‐assisted tools could be of effective application in the recognition of elite accessions with the proper value. These profitable markers can be employed for high‐quality selection, especially when no genetic sequence map and identified gene loci are available for the species [75] . Besides, the combined data analysis indicated that there are four main clusters for the whole studied accessions ( Figure S3 ).…”

Section: Resultsmentioning

confidence: 99%

Association of Genetic Structure and Diversity in Iranian Wild Germplasms ofMentha longifoliaL. Based on Phenotypical, Biochemical, and Molecular Markers

Araghi

Nemati

Azizi

et al. 2021

Chemistry & Biodiversity

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Mentha longifolia L. is well‐known to be one of the most pervasive wild‐growing species of the Lamiaceae family, which has extensive beneficial properties in the fields of pharmacology and biological products. In the present study, the correlation between Inter‐simple sequence repeat (ISSR) markers and morpho‐chemical parameters of twenty different M. longifolia accessions (MLACs) were assessed. The geographic information system (GIS) has been employed to interpret the original habitat of the accessions in Iran. ISSR analysis indicated a remarkable difference in the studied accessions, segregated them into three main groups, constructed by an unweighted pair‐group method with arithmetic (UPGMA) and principal coordinate analysis (PCoA). A total of 89 bands were generated by 12 ISSR primers, among which 82 (91.97 %) of them were polymorphic. The cluster analysis based on agro‐morphological data scattered MLACs into two main groups. The essential oils (EOs) were analyzed through GC/FID/MS, and four chemotypes were characterized according to the major constituents. Pulegone ranged from 0.17 to 69.50 % was the main oil constituent with the highest content. Also, HPLC‐PDA was employed to identify and to quantify the phenolic compounds in the MeOH extracts of MLACs. Heatmap cluster based on phenolic compounds produced three main categories of accessions. The components identified in the extracts were rosmarinic acid, rutin, vanillic acid, ferulic acid, chlorogenic acid, caffeic acid, 3,4‐dihydroxybenzoic acid, 2,5‐dihydroxybenzoic acid, and p‐coumaric acid, which among them rosmarinic acid (RA) varied from 39.16 to 261.55 mg/100 g (DW) as a predominant constituent. Subsequently, multiple regression analyses between ISSR fragments and morpho‐chemical data illustrated considerable relationships in the plant materials. The high variation and correlation observed in metabolic and phenotypic traits of MLACs establish an adequate source to conduct reserves conservation programs.

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Section: Resultsmentioning

confidence: 99%

Association of Genetic Structure and Diversity in Iranian Wild Germplasms ofMentha longifoliaL. Based on Phenotypical, Biochemical, and Molecular Markers

Araghi

Nemati

Azizi

et al. 2021

Chemistry & Biodiversity

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“…In comparison to these classical approaches, a molecular marker based approach is much more accurate, precise and simple and has efficiently characterized genetic variability both at inter-specific, as well as intra-specific levels (Gepts 2002), and provide important directives for development of species specific markers (Tong-Jian et al 1991;Nekrutenko et al 2000). Thus, in order to analyze the genome diversity of RET category plants including orchids, molecular markers such as RAPD, ISSR, AFLP have been used routinely (Sharma et al 2011;Manners et al 2013;Bhattacharyya et al 2013Bhattacharyya et al , 2017Bhattacharyya and Van Staden 2016). These approaches are pragmatic and useful for designing conservation of plant genetic resources which are facing the risk of habitat fragmentation and extinction.…”

Section: Discussionmentioning

confidence: 99%

“…As the molecular markers are not influenced by any physical or biochemical factors, they help in a vivid manner in cultivar identification in early developmental stages (Chen et al 2009;Yu et al 2009;Cabo et al 2014). In the recent past, extensive research has been carried out on the aspects of genetic diversity in various orchid species at both interspecific, as well as intra-specific, levels with special reference to the genus Dendrobium (Sharma et al 2011;Manners et al 2013;Bhattacharyya et al 2013Bhattacharyya et al , 2017. However, till date there exists no scientific documentation of genetic diversity in any species of Eulophia.…”

Section: Introductionmentioning

confidence: 99%

Molecular insights into genetic diversity and population dynamics of five medicinal Eulophia species: a threatened orchid taxa of Africa

Bhattacharyya

Staden

2018

Physiol Mol Biol Plants

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Genetic diversity existing amongst five orchid species were assessed using start codon targeted polymorphism (SCoT) and inter-retrotransposon amplified polymorphism (IRAP) markers. A total of 12 SCoT and 5 IRAP markers revealed an average of 63% genetic variability [SCoT = 63.87; IRAP = 64.95%] amongst the five species investigated. The genetic similarities were assessed using both UPGMA and Bayesian approaches which indicated identical clustering patterns at a genetic similarity level of 50%. Analysis of molecular variance (AMOVA) revealed the presence of a significant degree of genetic variability, mostly compartmentalized within the species level. Amongst the five assessed species, was the most genetically diverse representative whereas was found to be least diverse based on a comparative assessment of various population genetic parameters like Nei's gene diversity (h) and Shannon's information index (I) with an overall gene flow value greater than 1. In order to evaluate the comparative marker efficiency, SCoT and IRAP marker data were subjected to various benchmark analyses like marker index, resolving power, polymorphic index content, multiplex ratio and effective multiplex ratio which revealed the robustness of both the marker techniques in assessment of genetic diversity. The present report provides the first molecular insights into the aspects of inter and intra specific genetic variability in medicinally as well as horticulturally important species along with addressing their conservation concerns. In a nutshell, the present approach is simple, rapid and cost effective and can be extended for analysis of genetic diversity of other related plant species.

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“…These genes will give an insight into homology, convergence as it is believed that these genes may be species specific 8 . Using PCR (Thermo Scientific make) based techniques the detection of intra and inter specific genetic variations can be done that helps in the identification 16 . These techniques help in evaluating the polymorphisms in the species even with the little differences 7 .…”

Section: Molecular Identificationmentioning

confidence: 99%

Isolation and Molecular Characterization of Thermophilic Coprophilous Fungus Malbranchea cinnamomea GSMBKU from Goat Dung

Ajmera¹,

Vamkudoth²,

Ranipadmini³

et al. 2019

J Pure Appl Microbiol

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Genetic variability and association of AFLP markers with some important biochemical traits in Dendrobium thyrsiflorum, a threatened medicinal orchid

Cited by 23 publications

References 72 publications

Association of Genetic Structure and Diversity in Iranian Wild Germplasms ofMentha longifoliaL. Based on Phenotypical, Biochemical, and Molecular Markers

Association of Genetic Structure and Diversity in Iranian Wild Germplasms ofMentha longifoliaL. Based on Phenotypical, Biochemical, and Molecular Markers

Molecular insights into genetic diversity and population dynamics of five medicinal Eulophia species: a threatened orchid taxa of Africa

Isolation and Molecular Characterization of Thermophilic Coprophilous Fungus Malbranchea cinnamomea GSMBKU from Goat Dung

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