2023
DOI: 10.3390/v15071617
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Genetic Variability and Molecular Evolution of Tomato Mosaic Virus Populations in Three Northern China Provinces

Abstract: RNA viruses tend to mutate during transmission and host infection, which is critical to viral adaptation and evolution. Tomato mosaic virus (ToMV) is a member of the genus Tobamovirus (family Virgaviridae) and an economically important virus with detrimental effects on tomatoes worldwide. Although the ToMV gene sequences have been completed in China, their genetic diversity and population structure remain unclear. We collected 425 tomato samples from tomato-growing areas in three northern Chinese provinces 201… Show more

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Cited by 4 publications
(3 citation statements)
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“…The phylogenetic analysis provides insights into the global diversity of ToMV strains. The majority of the strains cluster within a primary clade, which is consistent with the findings reported by Lyu et al [ 34 ]. Notably, the isolates INIFAP JM1–3 form a distinct group.…”
Section: Discussionsupporting
confidence: 92%
See 1 more Smart Citation
“…The phylogenetic analysis provides insights into the global diversity of ToMV strains. The majority of the strains cluster within a primary clade, which is consistent with the findings reported by Lyu et al [ 34 ]. Notably, the isolates INIFAP JM1–3 form a distinct group.…”
Section: Discussionsupporting
confidence: 92%
“…For ToMV, the 3′ end was additionally amplified with RT-PCR and Sanger sequencing. The RT-PCR was performed on each of the 36 samples using the specific direct primer ToMV_GSPD1 (5′-GGTTGCAATTCGGTCTGCTAT-3′) designed from the assembled contigs and the reverse primer ToMV_3R from the study by Lyu et al [ 34 ], with a 16 (T > A) base substitution (3′-TTATATATGGGCCCCTACCG-5′). These primers flank a 124 nt region of the coat protein (CP) end and the complete 3′ untranslated region (UTR) of ToMV.…”
Section: Methodsmentioning
confidence: 99%
“…To determine the viral particle concentration in infected plants, researchers employ various methods. One approach involves infecting indicator plants and observing the development of mosaic-like or necrotic spots on their leaves, which vary depending on the viral species [28]. For instance, Nicotiana glutinosa L. leaves are used to identify and measure potato virus concentrations, while young tobacco plants (Nicotiana glutinosa) serve as indicators for tomato aspermy virus.…”
Section: A Comprehensive Overview Of Visualizing Infected Plants and ...mentioning
confidence: 99%