Genetic variability and pathogenicity potential of Escherichia coli isolated from recreational water reservoirs

Orsi, Renato H.; Stoppe, Nancy C.; Sato, Maria Inês Z.; Gomes, Tânia A. T.; Prado, Paulo Inácio; Manfio, Gilson Paulo; Ottoboni, Laura Maria Mariscal

doi:10.1016/j.resmic.2007.02.009

Cited by 30 publications

(22 citation statements)

References 21 publications

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“…MLST-based phylogenetic analysis showed that plantassociated strains cover most of E. coli diversity, with the exception of the recently described phylogroup F. However, as observed in other environments (Walk et al, 2007;Orsi et al, 2007b;2007a;Ratajczak et al, 2010;Bergholz et al, 2011;Cook et al, 2011), we found an unequal phylogenetic distribution with a prevalence of B1 isolates, suggesting that the E. coli phylogroups are not equivalent in their ecology and that B1 may be different (O'Brien and Gordon, 2011). By combining phenotypic and phylogenetic characterization we could then reveal that phenotypes discriminating plant-and host-associated strains were phylogenetically distributed.…”

Section: Discussionmentioning

confidence: 90%

Phylogenetic distribution of traits associated with plant colonization in Escherichia coli

Méric

Kemsley

Falush³

et al. 2012

Environmental Microbiology

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Plants are increasingly considered as secondary reservoirs for commensal and pathogenic Escherichia coli strains, but the ecological and functional factors involved in this association are not clear. To address this question, we undertook a comparative approach combining phenotypic and phylogenetic analyses of E. coli isolates from crops and mammalian hosts. Phenotypic profiling revealed significant differences according to the source of isolation. Notably, isolates from plants displayed higher biofilm and extracellular matrix production and higher frequency of utilization of sucrose and the aromatic compound p-hydroxyphenylacetic acid. However, when compared with mammalian-associated strains, they reached lower growth yields on many C-sources commonly used by E. coli. Strikingly, we observed a strong association between phenotypes and E. coli phylogenetic groups. Strains belonging to phylogroup B1 were more likely to harbour traits indicative of a higher ability to colonize plants, whereas phylogroup A and B2 isolates displayed phenotypes linked to an animal-associated lifestyle. This work provides clear indications that E. coli phylogroups are specifically affected by niche-specific selective pressures, and provides an explanation on why E. coli population structures vary in natural environments, implying that different lineages in E. coli have substantially different transmission ecology.

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Section: Discussionmentioning

confidence: 90%

Phylogenetic distribution of traits associated with plant colonization in Escherichia coli

Méric

Kemsley

Falush³

et al. 2012

Environmental Microbiology

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“…The declining rate of production of new antimicrobials and an increase in MDR pathogens in the clinic and community is precipitating a global health crisis [77]. Continuous migration and international travel have led to increased antimicrobial resistance in this group of bacteria due to mobile genetic elements that are easily spread through direct contact and ingestion of contaminated water or food [29,78,79]. It is therefore necessary to screen outpatients for asymptomatic bacterial infections and also food animal handlers before they handle animal food products sold to the community to prevent transmission or circulation of clonal strains of MDR E. coli.…”

Section: Discussionmentioning

confidence: 99%

Prevalence and multidrug resistance of Escherichia coli from community-acquired infections in Lagos, Nigeria

Adenipekun

Jackson

Ramadan

et al. 2016

J Infect Dev Ctries

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Introduction: The emergence of multidrug resistance (MDR; resistance to ≥ 2 more antimicrobials) in Escherichia coli is of concern due to complications encountered in treatment. Methodology: In this study, prevalence, antimicrobial resistance, and genetic characteristics of MDR community isolates of E. coli from Lagos, Nigeria were determined. Urine and stool samples were obtained from outpatients attending Lagos State hospitals and from animal handlers in abattoirs, poultries, and open markets, from December 2012 to July 2013.Results: Approximately 50% of urine (200/394) and 88% of stool samples (120/136) were positive for E. coli. Based upon β-lactamase production, a subset of those isolates was selected for further study. Of the 22 antimicrobials tested, E. coli exhibited resistance to all antimicrobials except amikacin and piperacillin/tazobactam. The highest levels of resistance were to tetracycline (182/247; 73.7%), trimethoprim/sulfamethoxazole (152/247; 61.5%), and ampicillin (147/247; 59.1%). Resistance to the cephalosporins ranged from 1.6%-15% including the third-and fourth-generation cephalosporins, cefpodoxime (20/247; 8.1%) and cefepime (4/247; 1.6%), respectively. MDR was observed in 69.6% (172/247) of the isolates. Forty-eight E. coli resistant to at least five antimicrobials were selected for further analysis using pulsed-field gel electrophoresis; seven distinct clusters were observed among the diverse patterns. Of the 48 MDR E. coli, 30 different sequence types (ST) were detected using multilocus sequence typing, including four ST131. Conclusions: This study demonstrated circulating MDR E. coli in the Nigerian community. Monitoring of antimicrobial resistance in developing countries is necessary to optimize empiric treatment and the prudent use of antimicrobials.

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“…The detection of specific virulence attributes of a given strain allows for the determination of potential reservoirs of virulence genes, which are expected to play a key role as the origin of emerging diseases caused by pathogenic E. coli (Orsi et al 2007). Virulence gene detection is also a useful tool in the analysis and detection of new E. coli strains (Kuhnert et al 2000).…”

Section: Discussionmentioning

confidence: 99%

Antibiotic resistance profiles of Escherichia coli isolates from river sources in Durban, South Africa

Olaniran

Naicker

Pillay

2009

World J Microbiol Biotechnol

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Lack of access to potable water has forced many inhabitants of informal settlements in South Africa to rely on surface water sources for their daily water needs, thus exposing these communities to microbial contamination that can result in water-borne diseases. These water sources also serve as natural habitats of pathogenic E. coli strains which harbour virulence factors, which could play a role in the disease process, as well as various multi-drug resistant water-borne pathogens. This study investigated the microbiological quality of two river waters in Durban, South Africa, using total coliform and faecal coliform population as indices. The virulence markers and antibiogram profiles of the E. coli isolates from these rivers were also determined. The results indicated that water from these river sources were of poor microbiological quality and unfit for human consumption. Antibiotic Resistance Profiles of the isolates revealed that 97.1% of the Palmiet River isolates and 71.15% of the Umgeni River isolates were multiresistant to the antibiotics tested, with all the isolates found to be resistant to novobiocin. Characterization of the virulence markers revealed the presence of stx1, cnf1 and eaeA genes, indicating the possible health risk associated with the ingestion of water from these rivers. The inherent health risks associated with the use of these river water emphasises the need for safe water supply and provision of proper sanitation facilities for the inhabitants of the informal settlements along these river banks.

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Genetic variability and pathogenicity potential of Escherichia coli isolated from recreational water reservoirs

Cited by 30 publications

References 21 publications

Phylogenetic distribution of traits associated with plant colonization in Escherichia coli

Phylogenetic distribution of traits associated with plant colonization in Escherichia coli

Prevalence and multidrug resistance of Escherichia coli from community-acquired infections in Lagos, Nigeria

Antibiotic resistance profiles of Escherichia coli isolates from river sources in Durban, South Africa

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