Genetic variability and phylogenetic analysis of hosta virus X

Fajolu, O. L.; Wen, R.-H.; Windham, Alan S.; Windham, Mark T.; Moulton, John K.; Hajimorad, M. R.

doi:10.1007/s00705-009-0522-6

Cited by 10 publications

(15 citation statements)

References 24 publications

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“…It is not likely that the CymMV subgrouping based on nt sequences reflects specific host-plant features, since phylogenetic tree analysis showed no correlation between isolates of each subgroup and plant species that they were isolated from or between isolates of each subgroup and the geographical origins. In accordance with these observations, the clustering of CymMV isolates confirmed previous CP analysis data for the trees obtained from the CP alignment of CymMV or other potexviruses [22][23][24]. Some isolates of CymMV showed significantly variability at the C-termini of the CP.…”

Section: Discussionsupporting

confidence: 89%

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Genetic variability in the coat protein genes of Cymbidium mosaic virus isolates from orchids

et al. 2011

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The variability in the nucleotide (nt) and amino acid (aa) sequences of the coat protein (CP) of Cymbidium mosaic virus (CymMV), which naturally infects orchids worldwide, was investigated. The CP genes of 55 CymMV isolates originating from different locations in Korea were amplified using RT-PCR and sequenced. The encoded CP consists of 223 aa. The CP sequences of the Korean isolates were compared with those of previously published CymMV isolates originating from different countries at both nt and aa levels. The Korean isolates shared 74.9-98.3 and 52.7-100% CP homology with CymMV isolates from other countries at the nt and aa levels, respectively. No particular region of variability could be found in either grouping of viruses. In the deduced CymMV CP aa sequence, the C-terminal region was more divergent than the N-terminal. The phylogenetic tree analysis based on nt sequence diversity of CP genes of CymMV isolates supported the hypothesis that CymMV isolates were divided into two subgroups. However, these subgroups were not formed by phylogenetic tree analysis of CP aa sequences. There was no distinct correlation between geographical locations and specific sequence identity, while recombination analysis revealed that there were no intra-specific recombination events among CymMV isolates.

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Section: Discussionsupporting

confidence: 89%

“…Some isolates of CymMV showed significantly variability at the C-termini of the CP. A similar observation was found in the CP analysis of Hosta virus X (HVX), which showed that the C-terminal region of the CP in HVX isolates is the most variable region of this protein [24].…”

Section: Discussionmentioning

confidence: 54%

Genetic variability in the coat protein genes of Cymbidium mosaic virus isolates from orchids

et al. 2011

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“…This indicated that PVX was under strong selection pressure against changes in the structures of PVX proteins and even nt sequences. A similar situation occurs in the case of another potexvirus, hosta virus X, whose isolates share identities of 98.3-100% at nt level and 98.6-100% at aa level [23]. Therefore, the variability of viruses transmitted via infected vegetative propagating materials may not be higher than aphid-borne or seed-borne viruses.…”

Section: Discussionmentioning

confidence: 87%

Phylogenetic analyses of an isolate obtained from potato in 1985 revealed potato virus X was introduced to China via multiple events

Jia

Zhang

et al. 2010

Virus Genes

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Potato virus X (PVX) is one of the most common plant viruses that cause great economic losses to solanaceous plants. We have previously reported the complete genomic sequence of the 2006 Chinese potato isolate FX21 and demonstrated that PVX isolates cluster into two groups: Eurasia and America. Here, we present the complete genomic sequence of one PVX isolate collected from potato in 1985 (PVX-1985). The genome of PVX-1985 is identical to that of FX21 in length and has the same genomic structure. PVX-1985, which like FX21 fell within the Eurasia group, clustered together with isolates from Europe, whereas FX21 clustered together with isolates of primarily Asian origin. Phylogenetic analyses of the complete genomic sequences and of CP gene sequences showed that Chinese PVX isolates have different origins and were introduced via multiple events. Though all the open reading frames of PVX are under negative/purifying selection, the central region of RNA-dependent RNA polymerase is under positive/diversifying selection.

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“…HVX has been subsequently reported in Korea (Ryu et al 2002), the Netherlands (Verhoeven et al 2006), Poland (Cajza and Zielinska 2007) and France (Jullien and Chauvel 2007) and has become a problem to hosta growers. HVX has been confirmed to be a distinct species of the genus Potexvirus in the family Flexiviridae (Park and Ryu 2003;Fajolu et al 2009). In nature, this virus infects only hostas and is most likely to be spread by vegetative propagation or mechanical contact (Currier and Lockhart 1996), and through seed (Ryu et al 2006).…”

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confidence: 98%