Genetic variability and virulence of Meloidogyne incognita populations from Brazil to resistant cotton genotypes

Silva, Esdras Henrique da; Mattos, Vanessa da Silva; Furlaneto, Cleber; Giband, Marc; Barroso, Paulo Augusto Vianna; Moita, A. W.; Aldemiro, Jorge-Junior; Correa, Valdir R.; Castagnone‐Sereno, Philippe; Carneiro, R. M. D. G.

doi:10.1007/s10658-014-0381-1

Cited by 15 publications

(17 citation statements)

References 28 publications

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“…Differences in the virulence of M. incognita isolates on resistant cotton genotypes have been demonstrated by Ogallo et al (1997), Zhou et al (2000), Anwar and McKenry (2007) and Silva et al (2014). Different strategies have been used for assessing resistance to M. incognita in cotton germplasm collections.…”

Section: Discussionmentioning

confidence: 98%

Reaction of Cotton Genotypes to Meloidogyne Incognita Race 3 and Selection Ofisolates With High Reproductive Rate in Cotton

Pires

Antes²,

Domiciano

et al. 2020

Preprint

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Background: Among the pathogens that reduce cotton productivity in Brazil, Meloidogyne incognita is one of the most important as it causes severe yield losses and is widespread. The most recommended methods for control of this species are the use of resistant cultivars and crop rotation systems. In Brazil, M. incognita races 3 and 4 have already been reported as cotton parasites but the race 3 is the most commonly found and widely disseminated.This work aimed at selecting virulent and aggressive populations of M. incognita race 3 for testing sources of resistance to this nematode in cotton genotypes.Results: The three isolates of M. incognita race 3 were important for screening of resistant cotton genotypes. The isolate from Umuarama was the most aggressive followed by Moreira Sales and Iporã. The genotypes CD 05-419, CD 05-945, CD 05-1087 and CD 05-1170 showed good performance against M. incognita race 3 in both greenhouse and field conditions.Conclusions: The cotton genotypes CD05-945, CD05-1170, CD05-1087 and CD05-419 will be selected for future work involving tests for resistance against other important cotton pathogens. Those genotypes can also be used as source of genes for resistance to nematodes in cotton breeding programs. The search for virulent and aggressive M. incognita isolates was very important when screening for resistance in cotton germplasm.

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Section: Discussionmentioning

confidence: 98%

Reaction of Cotton Genotypes to Meloidogyne Incognita Race 3 and Selection Ofisolates With High Reproductive Rate in Cotton

Pires

Antes²,

Domiciano

et al. 2020

Preprint

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“…Although the terminology 'races' of Meloidogyne spp. has been recommended not to be used since 2009 [2], several papers related to M. incognita diversity of host compatibility or selection of resistant cultivars are still using this term; including on coffee [55,56]; cotton [57,58] or soybean [59]. This reflects the practical importance to differentiate M. incognita populations according to their different ranges of host compatibilities.…”

Section: Discussionmentioning

confidence: 99%

Population genomics supports clonal reproduction and multiple gains and losses of parasitic abilities in the most devastating nematode plant pest

Koutsovoulos

Marques

Arguel

et al. 2018

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17The most devastating nematodes to worldwide agriculture are the root-knot nematodes with 18Meloidogyne incognita being the most widely distributed and damaging species. This parasitic 19 and ecological success seem surprising given its supposed obligatory clonal reproduction. Clonal 20 reproduction has been suspected based on cytological observations but, so far, never confirmed 21 by population genomics data. At the species level, M. incognita is highly polyphagous with 22 thousands of host plants. However, the host range varies among different M. incognita isolates 23 that may present distinct and more restricted host compatibilities. Historically, four 'host races' 24 had been defined as a function of ranges of compatible and incompatible plants. We sequenced 25 the genomes of 11 isolates across Brazil, covering these four distinct races to assess (i) how clonal 26 reproduction is and (ii) how the level of genome variability associates with biological traits such 27 as the host races, affected agronomic culture, and geographical distribution. By aligning the 28 genomic reads of the isolates to the M. incognita reference genome assembly, we identified SNPs 29 and small-scale insertions/deletions. Analysis of linkage disequilibrium and 4-gametes test, 30 showed no sign of recombination, confirming the clonal mode of reproduction of M. incognita. 31We showed that there are relatively few point variations between the different isolates, and these 32 variations show no significant association with either the host races, the geographical origin of 33 the samples or the host plant on which they have been collected. Due to the lack of phylogenetic 34 signal underlying their existence, we recommend the discontinuation of the terminology "race". 35Overall, these results suggest that multiple gains and losses of parasitic abilities and adaptations 36 to different environmental conditions account for the broad host spectrum and wide geographic 37 distribution of M. incognita. Hence, this metazoan constitutes a model species to study 38 adaptability without sexual recombination and overall low genomic variations in animals. 39 40

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“…The genetic diversity of Brazilian M. incognita populations was compared using populations from Paraná (PR) and Mato Grosso do Sul (MS) states, studied by Silva et al . (), and a population of M. enterolobii was included as an out‐group (Table ). RAPD reactions occurred in a volume of 13 μL containing 9 ng genomic DNA under the conditions described by Randig et al .…”

Section: Methodsmentioning

confidence: 99%

“…Bahia is the second largest cotton‐growing state in Brazil, with 94% of the production in the western region, and has suffered severe losses due to the attack of this nematode (CONAB, ). In Brazil, most commercial cotton cultivars are susceptible to this RKN species, and only a few are moderately resistant or resistant (Silva et al ., ).…”

Section: Introductionmentioning

confidence: 97%

“…New sources of resistance are desirable for the development of cultivars with higher levels of resistance to this RKN and less likely to be supplanted by variants of the pathogen. Recently, the accession of Gossypium barbadense (CIR1348) was identified as a new source of high resistance to M. incognita (Mota et al ., ; Silva et al ., ). In accession CIR1348 two QTLs were identified, one on chromosome 11 and another on chromosome 15, which are responsible for the high level of resistance to the nematode (Silva et al ., ; M. Giband, CIRAD, France, personal communication).…”

Section: Introductionmentioning

confidence: 97%

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Diversity of Meloidogyne incognita populations from cotton and aggressiveness to Gossypium spp. accessions

et al. 2019

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a Programa de P os-Graduac ßão em Fitopatologia, Universidade de Bras ılia, Bras ılia, DF 70910-900; b Embrapa Centro de Recursos Gen eticos e Biotecnologia, Bras ılia, DF 70849-970; c Embrapa Algodão,The root-knot nematode (RKN) Meloidogyne incognita is the main nematode causing losses to the cotton crop in Brazil. In order to implement control strategies within integrated management, an accurate identification of the nematode populations prevailing in the cotton production areas is necessary. This study aimed to assess the genetic variability and aggressiveness of RKN populations from cotton production areas in Bahia state, Brazil. All populations were characterized biochemically and molecularly and identified as M. incognita. RAPD and AFLP markers detected 44% of polymorphic fragments among the 13 populations of this species. The 10 M. incognita populations collected in Bahia presented 33.7% of diversity when compared to each other, and 25% when the population from Barreiras (the most polymorphic) was excluded. This polymorphism increased when populations from other Brazilian states were included. The aggressiveness and virulence among populations from Bahia towards different cotton accessions (susceptible/resistant) was also studied. None of the populations showed virulence against the moderately resistant (Clevewilt 6, Wild Mexican Jack Jones and LA 887) and highly resistant (CIR1348 and M-315 RNR) cultivars. Two M. incognita populations from Barreiras were the most aggressive, reaching reproduction factors of 539 and 218, respectively, in the susceptible cultivar FiberMax 966. The most aggressive population (8) was also the most genetically divergent in phylogenetic analyses. These results demonstrate that diversity of M. incognita populations from cotton farms in Bahia is not related to virulence against resistant accessions, which suggests that cultivars containing one or two resistance genes with good agronomic characteristics could be used in infested commercial areas in Bahia state, Brazil.

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Genetic variability and virulence of Meloidogyne incognita populations from Brazil to resistant cotton genotypes

Cited by 15 publications

References 28 publications

Reaction of Cotton Genotypes to Meloidogyne Incognita Race 3 and Selection Ofisolates With High Reproductive Rate in Cotton

Reaction of Cotton Genotypes to Meloidogyne Incognita Race 3 and Selection Ofisolates With High Reproductive Rate in Cotton

Population genomics supports clonal reproduction and multiple gains and losses of parasitic abilities in the most devastating nematode plant pest

Diversity of Meloidogyne incognita populations from cotton and aggressiveness to Gossypium spp. accessions

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