Genetic variability in Metarhizium flavoviride revealed by telomeric fingerprinting

Inglis, Peter W.

doi:10.1016/s0378-1097(99)00390-0

Cited by 4 publications

(6 citation statements)

References 14 publications

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“…Telomeric probes have previously been used for DNA fingerprinting of filamentous fungi such as U. maydis (Sanchez-Alonso et al 1996), Metarhizium sp. (Inglis et al 1999), Nomuraea rileyi (Boucias et al 2000), Cercospora caricis (Inglis et al 2001b) and Beauveria sp. (Viaud et al 1998).…”

Section: Discussionmentioning

confidence: 99%

“…Extraction of fungal genomic DNA from liquid-grown mycelium, and analysis of telomeric RFLPs were described previously (Inglis et al 1999). In order to map the extent of the element associated with the monomorphic telomeres in strain CG34, genomic DNA was digested with a range of restriction enzymes, and Southern blots were probed with the telomeric sequence from Fusarium oxysporum [TTAGGG] 18 (Powell and Kistler 1990).…”

Section: Telomere Fingerprintsmentioning

confidence: 99%

“…acridum (= M. flavoviride) isolates by restriction fragment length polymorphism (RFLP) using a telomeric probe (Inglis et al 1999), and found the telomere-associated region to be highly polymorphic. We have also used this technique to investigate genetic variability in our collection of entomogenous Communicated by E. di Mauro M. anisopliae var.…”

Section: Introductionmentioning

confidence: 99%

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Monomorphic subtelomeric DNA in the filamentous fungus, Metarhizium anisopliae,contains a RecQ helicase-like gene

et al. 2005

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In most filamentous fungi, telomere-associated sequences (TASs) are polymorphic, and the presence of restriction fragment length polymorphisms (RFLPs) may permit the number of chromosome ends to be estimated from the number of telomeric bands obtained by restriction digestion. Here, we describe strains of Metarhizium, Gliocladium and Paecilomyces species in which only one or a few telomeric bands of unequal intensity are detectable by Southern hybridization, indicating that interchromosomal TAS exchange occurs. We also studied an anomalous strain of Metarhizium anisopliae, which produces polymorphic telomeric bands larger than 8 kb upon digestion of genomic DNA with XhoI. In this case, the first XhoI site in from the chromosome end must lie beyond the presumed monomorphic region. Cloned telomeres from this strain comprise 18-26 TTAGGG repeats, followed at the internal end of the telomere tract by five repeats of the telomere-like sequence TAAACGCTGG. An 8.1-kb TAS clone also contains a gene for a RecQ-like helicase, designated TAH1, suggesting that this TAS is analogous to the Y' elements in yeast and the subtelomeric helicase ORFs of Ustilago maydis (UTASRecQ) and Magnaporthe grisea (TLH1). The TAS in the anomalous strain of M. anisopliae, however, appears distinct from these in that it is found at most telomeres and its predicted protein product possesses a significantly longer N-terminal region in comparison to the M. grisea and U. maydis helicases. Hybridization analyses showed that TAH1 homologues are present in all other anomalous M. anisopliae strains studied, as well as in some other polymorphic strains, where the recQ-like gene also appears to be telomere-associated.

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Section: Discussionmentioning

confidence: 99%

Section: Telomere Fingerprintsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Monomorphic subtelomeric DNA in the filamentous fungus, Metarhizium anisopliae,contains a RecQ helicase-like gene

et al. 2005

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“…Intra-specific genetic polymorphism in entomogenous fungi has facilitated strain characterization often employing a range of techniques (e.g. St Leger et al 1992;Pfeifer and Khachatourians 1993;Viaud et al 1996;Couteaudier and Viaud 1997;Maurer et al 1997;Inglis et al 1999;Enkerli et al 2001;Coates et al 2002a,b). Most often the markers can distinguish at the species but not at the strain level.…”

Section: Introductionmentioning

confidence: 99%

Molecular monitoring and evaluation of the application of the insect-pathogenic fungus Beauveria bassiana in southeast China

Wang

Meizhen

et al. 2004

J Appl Microbiol

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Aims: To monitor the fate of inundatively applied strains of Beauveria bassiana against Masson's pine caterpillar, Dendrolimus punctatus, in Magushan Forest, southeast China, and evaluate the potential environmental risks of biocontrol introduction. Methods and Results: Filed samplings of mycosed cadavers were carried out continuously for more than one year after the inundative applications of two exotic B. bassiana strains. By employing four complementary molecular markers, the purified cultures from collections were characterized and the two released strains Bb17 and Bb13 were recovered with respective frequencies of 9AE1 and 5AE2%. The released strains were isolated from nontarget hosts. The genetic diversity of the population of B. bassiana in the trial plot changed dynamically over time and no single genotype strain dominated throughout the sampling period or at different seasons. The indigenous strains were observed to be predominant in the local environment. Co-infection and/or genetic recombination might occur between strains under natural conditions. Conclusion: Field applications of the biocontrol agent B. bassiana appear to pose no major risk to the environment. There was no evidence of displacement of indigenous strains of B. bassiana. Significance and Impact of the Study: The results indicate that the introduction of exotic B. bassiana strains can persist in local environment and infect nontarget insect hosts, highlighting the importance of safety assessment of the large-scale application of fungal biocontrol agents.

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“…acridum isolates [7]. The same set of isolates, however, were found to be highly distinguishable by RFLP using a telomeric probe [8].…”

Section: Introductionmentioning

confidence: 88%

Biolistic co-transformation ofMetarhizium anisopliaevar.acridumstrain CG423 with green fluorescent protein and resistance to glufosinate ammonium

Inglis

Aragão

Frazão

et al. 2000

FEMS Microbiology Letters

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Metarhizium anisopliae var. acridum (syn. M. flavoviride) is recognized as a highly specific and virulent mycopathogen of locusts and grasshoppers and is currently being developed as a biological control agent for this group of insects in Brazil. Intact conidia of M. anisopliae var. acridum strain CG423 were transformed using microparticle bombardment. Plasmids used were: (1) pBARKS1 carrying the bar gene of Streptomyces hygroscopicus fused to the Aspergillus nidulans trpC promoter, encoding resistance to glufosinate ammonium (or phosphinothricin) and modified by addition of the telomeric repeat (TTAGGG) 18 of Fusarium oxysporum and 2.pEGFP/gpd/tel carrying a red-shifted variant gene for Aequorea victoria green fluorescent protein (EGFP) which we have fused to the A. nidulans gpd promoter and trpC terminator. Highly fluorescent co-transformants were selected on solid minimal medium containing 100 Wg ml 31 glufosinate ammonium using an inverted microscope with 450^490 nm excitation/510 nm emission filter set. Southern blot analysis of co-transformants revealed varying multiple chromosomal integrations of both bar and egfp genes at both telomeric and non-telomeric loci. Transformants retained pathogenicity in bioassays against Rhammatocerus schistocercoides and showed unaltered lack of pathogenicity against larvae of the non-target insect Anticarsia gemmatalis. One co-transformant from four tested, however, showed a significant, but non-dose-dependent, elevation in virulence against Tenebrio molitor. ß

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Genetic variability in Metarhizium flavoviride revealed by telomeric fingerprinting

Cited by 4 publications

References 14 publications

Monomorphic subtelomeric DNA in the filamentous fungus, Metarhizium anisopliae,contains a RecQ helicase-like gene

Monomorphic subtelomeric DNA in the filamentous fungus, Metarhizium anisopliae,contains a RecQ helicase-like gene

Molecular monitoring and evaluation of the application of the insect-pathogenic fungus Beauveria bassiana in southeast China

Biolistic co-transformation ofMetarhizium anisopliaevar.acridumstrain CG423 with green fluorescent protein and resistance to glufosinate ammonium

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