Genetic variability in sugarcane (Saccharum spp. hybrid) genotypes through inter simple sequence repeats (ISSR) markers

Abstract

In the present study, 14 sugarcane (Saccharum spp. hybrid) genotypes were used for genomic diversity analysis based on nineteen inter simple sequence repeat (ISSR). These nineteen sets of ISSR markers generated a total of 164 discernible and reproducible bands including 109 polymorphic and 55 monomorphic bands. The unweighted pair group method with arithmetic average (UPGMA) analysis revealed three distinct clusters: I, II and III within the 14 genotypes. The polymorphic information content (PIC) value per locus ranged from 0.14 (UBC811) to 0.53 (ISSR1) locus with an average of 0.42 for all loci. The range of genetic distance or coefficient of similarity among sugarcane genotypes varied 0.14 - 0.78. The analysis of these similarities matrix revealed that greater similarity between CoS03234 and CoSe1424 (0.78), and lowest similarity between CoS03234 and Co0118 (0.14). The knowledge gained in this study would be useful to future breeding programs for increasing genetic diversity of sugarcane varieties and cultivars to meet the increasing demand of sugarcane cultivation for sugar and bio energy uses.

Genetic diversity of Saccharum complex using ISSR markers

“…Devarumath et al (2012) found the production of 65 amplified fragments, with 96.5% polymorphism, in 13 ISSR primers used for PCR amplification. Rao et al (2016), for ISSR analysis, used 19 primers in 14 genotypes of sugarcane for amplification of the PCR, a total of 164 bands were marked with 109 polymorphic bands and 55 monomorphic bands. These results, as well as in this study, prove the efficiency of ISSR when compared with other markers, revealing a high degree of polymorphism.…”

“…The molecular markers are important tools for the breeding of plants and aim at the mapping of genes, genetic diversity analysis, disease diagnostics, and taxonomic and evolutionary studies (Wünsch and Hormaza, 2007). In sugarcane, some studies have been conducted to identify the genetic diversity among genotypes using AFLP (Selvi et al, 2006), RAPD (Khan et al, 2009;Ullah et al, 2013), chloroplast microsatellite markers (coSSR) (Raj et al, 2016), SSR (Pandey et al, 2011) and inter-simple sequence repeat (ISSR) (Srisvastava and Gupta, 2008;Almeida et al, 2009;Devarumath et al, 2012;Rao et al, 2016). RAPD and ISSR have been amplified for water stress tolerance in sugarcane varieties (Fahmy et al, 2008).…”

Genetic relationships in Saccharum complex germplasm collections based on morphological and molecular markers

“…Sugarcane breeders and geneticists have discovered the use of numerous DNA markers, including amplified fragment length polymorphisms (AFLP) (Caroll & Curtis, 1996;D'Hont & Glaszmann, 2001), restriction fragment length polymorphisms (RFLP) (Burnquist et al, 1995), single nucleotide polymorphism (SNP), simple sequence repeats (SSRs) (Pandey et al, 2011;Hameed et al, 2012), random amplification of polymorphic DNAs (RAPD) (Ullah et al, 2013), inter simple sequence repeat (ISSRs) (Devarumath et al, 2012;Rao et al, 2016;Olieviera et al, 2017), r-DNA-ITS sequence and expressed sequence tagsimple sequence repeat (EST-SSRs) to improve the Saccharum breeding (Ali et al, 2019). Among these molecular markers, SSR (microsatellite) markers have been widely used to study sugarcane genetic diversity, genetic mapping, crosstransferability, paternity analysis, segregation analysis, and marker-assisted selection.…”

Agro-morphological characteristics and genetic diversity of newly registered sugarcane variety Jitpur-5, Jitpur-6 & Jitpur-7 in Nepal.

“…Today's sugarcane are the complex hybrids obtained after crossing between diverse species of Saccharum (Srivastava and Gupta, 2008). The size and complexity of its genome is a major limitation in genetic improvement, however morphological traits, pedigree record and molecular markers can be used to estimate genetic diversity (Rao et al, 2016). For the country like Nepal, where breeding is just at a nascent stage, there is a great need for the evaluation of introduced sugarcane varieties for morphological characteristics for their identification, distinctiveness, uniformity and stability, for its use in breeding and selection of suitable canes for the commercial sugarcane production.…”