Genetic variability in the three subspecies of <i>Marchantia polymorpha (Hepaticae)</i>: isozymes, RFLP and RAPD markers

Boisselier‐Dubayle, Marie‐Catherine; Jubier, Marie-France; Lejeune, Bernard; Bischler, H.

doi:10.2307/1223406

Cited by 62 publications

(34 citation statements)

References 27 publications

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“…These loci were acknowledged as diagnostic markers in many studies (e.g. [11,21,22]). For the genus Calypogeia, the most effective marker was the fastest isozyme of esterase system (Est-1), which allows to identify all species occurring in Poland except for the pair C. suecica -C. neesiana [12].…”

Section: Discussionmentioning

confidence: 99%

New taxon of the genus Calypogeia (Jungermanniales, Hepaticae) in Poland

Buczkowska

Bączkiewicz

2011

Acta Soc Bot Pol

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Genetic differentiation of Calypogeia muelleriana s.l. was studied using isozyme analysis. Two forms of this species: typical and atypical were reported from Poland. The 10 putative loci in 7 enzyme systems were analyzed in 58 samples: 34 of the typical and 15 of atypical form. The isozyme studies revealed that the typical and atypical forms of C. muelleriana in Poland are clearly genetically different. Typical plants morphologically correspond to the type specimen of C. muelleriana, but atypical form is a new, genetically distinct but unrecognized so far taxon. Each group is defined by several fixed alleles present in all populations. The UPGMA dendrogram based on Nei's genetic distances shows that both taxa (C. muelleriana and the newly detected taxon) clearly differ from C. azurea -the species used as a reference group. Genetic distance among two groups of C. muelleriana (D = 1.093) was almost the same as among C. azurea and the newly detected taxon (D = 1.060). Genetic distance among C. azurea and the typical form of C. muelleriana was the lowest (D = 0.628).

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Section: Discussionmentioning

confidence: 99%

New taxon of the genus Calypogeia (Jungermanniales, Hepaticae) in Poland

Buczkowska

Bączkiewicz

2011

Acta Soc Bot Pol

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“…2, 4B). The first study based not only on isozymes but also DNA fingerprints, was performed by Boisselier-Dubayle et al (1995) to distinguish subspecies within Marchantia polymorpha, using RAPD (random amplified polymorphic DNA) and RFLP (restriction fragment length polymorphism) techniques, which were the first DNA fingerprinting techniques available. In particular RAPD became widely used, including in bryophytes, for some time in the 1990s.…”

Section: Fingerprinting Methodsmentioning

confidence: 99%

20,000 species and five key markers: The status of molecular bryophyte phylogenetics

Stech¹,

Quandt²

2014

Phytotaxa

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A number of reviews have accompanied and monitored the progress of molecular phylogenetic research on bryophytes, focusing on the publication record itself, bryophyte phylogeny and systematics in the molecular era, as well as the evolution and phylogenetic utility of markers from different genomes. However, none of the recent reviews include a detailed characterization of all molecular markers used in bryophyte phylogenetics. Here we provide an overview of the history and current state of marker utilization, including coding and non-coding sequence markers from all three genomes as well as fingerprinting approaches. The molecular architecture and evolutionary peculiarities, as well as practical aspects such as amplification and sequencing strategies, are outlined for the DNA sequence markers, with a focus on the most commonly employed regions. Their phylogenetic utility and potential for solving some of the remaining, pressing questions in bryophyte phylogeny, as well as their suitability for molecular species identification by DNA barcoding, are discussed.

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“…Markers of this class were highly effective in distinguishing between the morphologically similar S. capillifolium and S. rubellum species, revealing five species-specific bands for each. RAPD markers were also successfully used in identifying the species of the genus Fossombronia [30] as well as the sibling species of Marchantia polymorpha [53]. RAPD markers are often criticized for low replicability [28,29].…”

Section: Discussionmentioning

confidence: 99%

A comparison of PCR-based markers for the molecular identification of Sphagnum species of the section Acutifolia

Sawicki

Szczecińska

2011

Acta Soc Bot Pol

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IntroductionDue to their great phenotypic plasticity, the classification of peat moss species often requires microscopic identification, which is both labor-and time-consuming. In addition to a standard morphological description, the classification of bryophytes increasingly often involves the use of molecular markers [1,2]. Chloroplast and nuclear genomes are mainly used, but this approach implies high analysis costs. Species-specific molecular markers support material identification even when diagnostic traits cannot be observed. The above applies mostly to traits related to sexual reproduction (gametengia, sporophytes) and vegetative reproduction (gemmae) which are often undeveloped or already degraded.The identification of peat moss species possess some problems. According to various authors, their number ranges from 39 [3] to 340 [4]. The main reason for such a wide variation in the reported number of Sphagnum species is their high phenotypic plasticity. Mosses growing in wet habitats exhibit high morphological variability in response to water level fluctuations and changes in other environmental conditions [5]. Another factor that affects morphological variability and hinders the species classification of peat mosses is hybridization [6][7][8][9].Isoenzymatic electrophoresis is a rapid and relatively inexpensive method of molecular species identification. The use of markers of this class permitted the molecular identification of the majority of morphologically controversial species of the genus Sphagnum [10,11]. However, their practical application is difficult, as in order to obtain stable and distinct isoenzymatic patterns, plants should be grown under glass-house conditions to normalize their expression levels and increase vitality [12]. Electrophoresis of enzymatic proteins cannot be applied to herbaceous materials, either, which makes it useless as a tool for verifying the correctness of species identification. Therefore, other molecular markers enabling a rapid, reliable and inexpensive identification have to be found.PCR-based DNA analyses provided new insights into the taxonomy of peat mosses. Studies of the genus Sphagnum rely primarily on RAPD [13,14], ISSR [9,15] and SSR [16] markers, and ITS sequences [17,18]. With a few exceptions [19], the above markers have not been used for molecular species identification.The objective of the present study was to evaluate the suitability of five types of DNA markers for molecular species identification. Peat moss species of the section Acutifolia were analyzed. Apart from markers that are commonly applied in studies of the genus Sphagnum, i.e. RAPD, ISSR and nuclear ITS regions, we also tested ISJ markers and primers complementary to the katG gene, which had been successfully used as species-specific markers in higher plants [20] The nuclear ITS region, analyzed based on the polymorphism of restriction loci [24,25] and a direct comparison of sequences [18,26], is widely used in the taxonomic studies of bryophytes. An extensive review of ITS applications i...

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Genetic variability in the three subspecies of Marchantia polymorpha (Hepaticae): isozymes, RFLP and RAPD markers

Cited by 62 publications

References 27 publications

New taxon of the genus Calypogeia (Jungermanniales, Hepaticae) in Poland

New taxon of the genus Calypogeia (Jungermanniales, Hepaticae) in Poland

20,000 species and five key markers: The status of molecular bryophyte phylogenetics

A comparison of PCR-based markers for the molecular identification of Sphagnum species of the section Acutifolia

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