Genetic variability in Tunisian populations of faba bean (Vicia faba L. var. major) assessed by morphological and SSR markers

Rebaa, Feten; Abid, Ghassen; Aouida, Marwa; Abdelkarim, Souhir; Aroua, Ibtissem; Muhovski, Yordan; Baudoin, Jean-Pierre; Mahmoud, Mahmoud A.; Sassı, Khaled; Jebara, Moez

doi:10.1007/s12298-017-0419-x

Cited by 27 publications

(28 citation statements)

References 53 publications

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“…The polymorphic information content which is a measure of allelic diversity based on allele frequency (Smith et al, 2000) ranged from 0.00 to 0.97 with a mean of 0.52 (Table 3). The mean PIC value in the current study was comparable to those reported in other cultivated legumes including common bean , soybean (Chauhan et al, 2015), cowpea (Ali et al, 2015), groundnut (Otenga-Frimpong et al, 2015) and faba bean (Rebaa et al, 2017). Three of the genomic microsatellites (e.g.…”

Section: Discussionsupporting

confidence: 86%

Genetic interrelationship among tepary bean (Phaseolus acutifolius A. Gray) genotypes revealed through SSR markers

Mhlaba¹,

Amelework²,

Shimelis³

et al. 2018

Aust J Crop Sci

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Tepary bean (Phaseolus acutifolius A. Gray) is one of the useful plant genetic resources possessing novel genes for abiotic and biotic stress tolerance. It is largely used in the breeding of common bean or related tropical legumes. The objective of this study was to determine the genetic interrelationship present among 20 diverse tepary bean genotypes using 10 selected and polymorphic simple sequence repeat (SSR) markers and to identify genetically unique parental lines for developing breeding populations. The SSR markers amplified a total of 57 putative alleles with a size range of 102 to 304 base pairs. Number of alleles ranged from 2 to 16 with a mean of 5.7 per locus. Number of effective alleles ranged from 1.5 to 11.6 with a mean of 4.32. The mean observed and expected heterozygosity were 0.45 and 0.51, respectively, reflecting moderate level of genetic interrelationship among the tested genotypes. The mean polymorphic information content (PIC) of the SSR loci was 0.50 suggesting that the selected markers had high discriminatory power for genetic diversity analysis of tepary bean. Genetic distances varied from 0.18 to 0.64 with a mean value of 0.42, signifying relatively low levels of genetic diversity among the studied genotypes. Cluster analysis grouped the genotypes into three distinct genetic groups. Unique genotypes such as G40201, G40237, G40158, G40157 and G40158 from Cluster I, G40084 and G40033 from Cluster III were selected. These are distantly related parents and recommended for further breeding or genetic recombination to broaden the genetic basis of tepary bean or related legumes.

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Section: Discussionsupporting

confidence: 86%

Genetic interrelationship among tepary bean (Phaseolus acutifolius A. Gray) genotypes revealed through SSR markers

Mhlaba¹,

Amelework²,

Shimelis³

et al. 2018

Aust J Crop Sci

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“…The results of critical analysis of seed color of faba bean germplasm accessions at the laboratory indicated least variation in seed coat color pattern but large variation seed size characters ( Table 2). These variations observed in seed size among the collected samples of indigenous Omani accessions are in conformity with those observed in previous studies that dealt with either local small or large germplasm collections [4,7,[17][18]. (Terzopolous et al, 2003;Kaur et al, 2014;Gasim et al, 2015;Rebaa et al, 2017).…”

Section: Discussionsupporting

confidence: 91%

“…These variations observed in seed size among the collected samples of indigenous Omani accessions are in conformity with those observed in previous studies that dealt with either local small or large germplasm collections [4,7,[17][18]. (Terzopolous et al, 2003;Kaur et al, 2014;Gasim et al, 2015;Rebaa et al, 2017). In the present study, villages located closely with the collecting sites appeared to have interestingly either similar patterns of seed coat color indicating the presence of large amount of homogeneity in the collected faba bean seed samples/ accessions whereas heterogenous seed samples were found in seed samples from three different sites.…”

Section: Discussionsupporting

confidence: 91%

Faba bean (<em>Vicia Faba </em>L.) Germplasm Collection and its Diversity in Oman

N¹,

Nadaf²,

Al-Lawati³

et al. 2018

Preprint

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Faba bean (Vicia faba L.) is one of the popular pulse crops grown for traditional consumption, not only in Oman but also in Arabian Peninsula and other countries. In Oman, the famers living in and around high altitude mountain areas mostly grow it. This paper highlights features of variation in seed color and diversity in seed traits found in collected accessions from the joint collecting missions of 2008-2010 between the staff of College of Agriculture, Sultan Qaboos University and the Ministry of Agriculture & Fisheries throughout all the governorates of the Sultanate of Oman. 41 seed samples/accessions were collected from 38 sites. Of these, the highest number of accessions was collected from Batinah South governorate (38) represented mostly by the mountains of Rustaq, followed by the mountains of Interior/Al-Dakhliyah (8), and Dhahira & Buraimi (4). Only one accession was collected from mountains of the Eastern/ Al-Sharqiyah governorates. The seed accessions were found diverse with respect to all seed characters studied, i.e. seed length (cm) and width (cm) and 1000-seed weight (g) besides seed color. The faba bean accessions were classified into 9 genetically diverse clusters based on the Principal Component Analysis, which indicated that the contribution of seed width and 100-seed weight to the total variation existing in indigenous germplasm collected from all the governorates of Oman. It was found from the critical analysis of seed colors that 38 accessions were homogenous (pure) with one color and three were heterogeneous with combinations of colors.

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“…The evaluation of genetic variability among faba bean landraces using agro-morphological and molecular markers revealed that sequence-related amplified polymorphism (SRAP) and amplified fragment length polymorphism (AFLP) markers are used to determine the genetic variability among genotypes (Ammar et al 2015;Backouchi et al 2015;Abid et al 2015;Rebaa et al 2017;Alemu et al 2018). AFLP markers and combined SRAP are considered to be remarkably beneficial for the evaluation of genetic variability at the molecular level.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the genetic variability of faba bean (Vicia faba L.) genotypes using agronomic traits and molecular markers

El-Shafei

Amer²,

El-Enany

et al. 2019

Bull Natl Res Cent

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Background: Breeding programs need to work with magnitudes of genetic variation in order to achieve the best results. In this study, we have estimated the genetic diversity and evaluated the field performance of 18 faba bean genotypes from different origins, during the 2014-2015 and 2015-2016 growing seasons. Results: The field evaluations revealed that the faba bean genotypes showed enough variation for their agronomic thoughtful parameters. ALB was noted as being the genotype that was the tallest (126.25 cm), but the superlative genotypes were L251 and L252 for a high number of seeds per plant (72.25) and the maximum seed yield per plant (56.40 g). The estimations of the genetic diversity between all of the faba bean genotypes were done using eight sequence-related amplified polymorphism (SRAP)-combined primers. The number of different alleles amplified for each polymorphic marker, ranged from 2 to 9, was observed by SRAP genotyping, with polymorphic information contents (PIC) between 0.445and 0.896, and discrimination power (DP) with an average of 0.125 per allele. Conclusions: Across both years, the best grain yields were gathered from the L25, L252, and L182 genotypes, in descending order, whereas the ALB genotype had the lowest grain yield in comparison with the other genotypes. The cluster analysis of faba bean genotypes was generally based on not only the origin but also the genetic background.

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Genetic variability in Tunisian populations of faba bean (Vicia faba L. var. major) assessed by morphological and SSR markers

Cited by 27 publications

References 53 publications

Genetic interrelationship among tepary bean (Phaseolus acutifolius A. Gray) genotypes revealed through SSR markers

Genetic interrelationship among tepary bean (Phaseolus acutifolius A. Gray) genotypes revealed through SSR markers

Faba bean (<em>Vicia Faba </em>L.) Germplasm Collection and its Diversity in Oman

Evaluation of the genetic variability of faba bean (Vicia faba L.) genotypes using agronomic traits and molecular markers

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