Genetic variability of Channa punctatus populations using randomly amplified polymorphic DNA

Nagarajan, Muniyandi; Haniffa, M. A.; Gopalakrishnan, A; Basheer, V. S.; Muneer, Abdul

doi:10.1111/j.1365-2109.2006.01539.x

Cited by 27 publications

(29 citation statements)

References 22 publications

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“…In conclusion, allozyme analysis revealed, as did the RAPD markers used by Nagarajan et al (2006), that of the three C. punctatus populations, the Tamirabarani and Kallada populations have similar genetic structures. The estimated values of average number of alleles, percentage of polymorphic loci, and heterozygosity for populations are considered indicators of the actual level of genetic variability of the species.…”

Section: Discussionmentioning

confidence: 67%

Allozyme Variation in a Threatened Freshwater Fish, Spotted Murrel (Channa punctatus) in a South Indian River System

et al. 2007

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Samples of the spotted murrel (Channa punctatus) were collected from three rivers of Tamil Nadu and Kerala. The allozyme variation of C. punctatus was investigated by polyacrylamide gel electrophoresis. Eighteen enzymes were detected, but only 10 (EST, PGM, G3PDH, G6PDH, SOD, GPI, ODH, GDH, XDH, and CK) showed consistent phenotypic variations. Allele frequencies were estimated at the 18 polymorphic loci representing 10 enzymes. Two rare alleles, EST-4*C and G6PDH-2*C, were noted in the Tamirabarani and Kallada populations but were absent in the Siruvani population. The allele frequencies of the Tamirabarani and Kallada populations were similar, except for a few loci. Among the three populations, the maximum genetic distance (0.026) and FST (0.203) were found between the geographically distant Siruvani and Kallada populations. Overall the study showed that among the three populations, the Tamirabarani and Kallada have similar genetic structures.

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Section: Discussionmentioning

confidence: 67%

Allozyme Variation in a Threatened Freshwater Fish, Spotted Murrel (Channa punctatus) in a South Indian River System

et al. 2007

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“…Firstly, heavy fishing pressure on the target species in these rivers may be one of the most important factors. Over-fishing is a severe problem during the past decades in India for unauthorized trade for aquarium keeping and this riverine habitat suffered from excessive exploitation and resources were severely declined [22,[56][57][58][59][60]. Extensive habitat alterations due to damming and deforestation, the proliferation of exotic species, and pollution, pesticides, and agrochemicals used in plantations are the other reasons.…”

Section: Discussionmentioning

confidence: 99%

Identification and characterization of microsatellite markers for the population genetic structure in endemic red-tailed barb, Gonoproktopterus curmuca

et al. 2014

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Gonoproktopterus curmuca is an endangered red tailed barb found in Southern part of Western Ghat, India. As a part of stock-specific, propagation assisted rehabilitation and management program, polymorphic microsatellites markers were used to study the genetic diversity and population structure of this species from the three River systems of Southern Western Ghats, such as Periyar River, the Chalakkudy River, and the Chaliyar River. From selected eight polymorphic microsatellite markers, the number of alleles per locus ranged from 2 to 8, and the average number of alleles among 3 populations ranged from 5.0 to 5.75. The mean observed (H ob ) and expected (H ex ) heterozygosity ranged from 0.5148 to 0.5360 and from 0.5996 to 0.6067, respectively. Significant deviations from Hardy-Weinberg Equilibrium expectation were found at majority of the loci (except Gcur MFW72 and Gcur MFW19) and in all three populations in which heterozygote deficits were apparent. The analysis of molecular variance indicates that the percent of variance among populations and within populations were 6.73 and 93.27, respectively. The pairwise F ST values between populations indicate that there were significant deviations in genetic differentiations for the red-tailed barb populations from these three Rivers of the Western Ghats, India. The microsatellites methods reported a low degree of gene diversity and lack of genetic heterogeneity in the population of G. curmuca, which strongly emphasize the need of fishery management, conservation and rehabilitation of G. curmuca.

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“…Populations GJ and MDJ showed the lowest genetic identity and their genetic relationship was the farthest. The population structure of freshwater organisms is primarily dependent on the distribution of river systems, as previously reported (Nagarajan et al, 2006;Zhuo et al, 2012). Genetic differentiation is primarily dependent on geographical isolation.…”

Section: Discussionmentioning

confidence: 74%

New microsatellite loci for the mandarin fish Siniperca chuatsi and their application in population genetic analysis

Tian¹,

Liang²,

Mu³

et al. 2014

Genet. Mol. Res.

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ABSTRACT. The mandarin fish is a popular fresh water food fish in China. Fifty-three polymorphic microsatellite markers were isolated through construction of an enriched library of genomic DNA of Siniperca chuatsi (Percichthyidae). We found 2 to 7 alleles per locus. The observed and expected heterozygosity values varied from 0.059 to 1.000 and from 0.305 to 0.818, respectively. The polymorphic information content value varied from 0.255 to 0.782. Twelve microsatellite loci deviated significantly from Hardy -Weinberg equilibrium after Bonferroni's correction. These markers were evaluated in five species of sinipercine fish; 98% of the 265 locus/taxon combinations tested gave crossamplification. Eight polymorphic microsatellite markers were randomly selected for genetic characterization of three S. chuatsi populations. The Ganjiang River and Yuanjiang River populations had moderate levels of genetic diversity, while the Mudanjiang River population had a relatively low level genetic diversity. Genetic distance-based cluster analysis showed clustering of the Ganjiang River and Yuanjiang River Microsatellite markers and mandarin fish genetic diversity populations in a single group and the Mudanjiang River population in a separate group. Based on these results, we suggest that S. chuatsi from the Yangtze River watershed are distinct from the Mudanjiang River population. These SSR markers will be useful for diversity, mapping and marker assisted studies of S. chuatsi and other sinipercine fishes.

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Genetic variability of Channa punctatus populations using randomly amplified polymorphic DNA

Cited by 27 publications

References 22 publications

Allozyme Variation in a Threatened Freshwater Fish, Spotted Murrel (Channa punctatus) in a South Indian River System

Allozyme Variation in a Threatened Freshwater Fish, Spotted Murrel (Channa punctatus) in a South Indian River System

Identification and characterization of microsatellite markers for the population genetic structure in endemic red-tailed barb, Gonoproktopterus curmuca

New microsatellite loci for the mandarin fish Siniperca chuatsi and their application in population genetic analysis

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