Genetic variability of farmed fur animals of the Canidae family assessed by nuclear and mitochondrial DNA analysis

Nisztuk-Pacek, Sylwia

doi:10.21521/mw.5544

Cited by 3 publications

(2 citation statements)

References 13 publications

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“…Canids (Canidae) is a family of carnivorous animals, including 16 genera and as many as 41 species (Nisztuk‐Pacek, ). All foxes belong to this family.…”

Section: Introductionmentioning

confidence: 99%

Assessment of chosen semen characteristics of two colour morphs of the Arctic fox Alopex lagopus L.

Stasiak

Kondracki

Iwanina

2019

Animal Science Journal

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The aim of the study was to evaluate semen quality in the two most popular colour morphs of the Arctic fox Alopex lagopus L., blue and white, based on ejaculate parameters, acrosin activity and analysis of sperm morphology. The research material consisted of ejaculates collected once by manual stimulation from 20 one‐year‐old male Arctic foxes (10 individuals of the blue morph and 10 of the white morph). Ejaculates were evaluated in terms of volume, sperm concentration, total number of spermatozoa and the percentage of spermatozoa with major and minor defects. The study revealed that male blue Arctic foxes produce ejaculates with much higher concentration (148.75 × 106/ml) and total number of spermatozoa (98.16 × 106) compared to white Arctic foxes (42.88 × 106/ml and 35.2 × 106 respectively). The level of acrosin activity from white foxes seemed to be higher compared to blue foxes but the difference was not statistically confirmed. Semen from Arctic foxes is characterized by high inter‐individual variability in sperm morphology. The frequency of morphological changes in sperm from Arctic foxes does not significantly depend on ejaculate volume, sperm concentration or the total number of spermatozoa in the ejaculate, but is associated with acrosin activity.

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“…Canids (Canidae) is a family of carnivorous animals, including 16 genera and as many as 41 species (Nisztuk‐Pacek, ). All foxes belong to this family.…”

Section: Introductionmentioning

confidence: 99%

Assessment of chosen semen characteristics of two colour morphs of the Arctic fox Alopex lagopus L.

Stasiak

Kondracki

Iwanina

2019

Animal Science Journal

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“…Consequently, the mitochondrial genome is a single haplotype inherited almost exclusively in the maternal line (Slaska and Grzybowska-Szatkowska 2011). Furthermore, it was found that the frequency of polymorphisms in the mitochondrial genome in the raccoon dog was twenty-fold higher than that in the nuclear genome (Nisztuk-Pacek 2016). With these features, mtDNA is often used in phylogenetic studies, molecular ecology, and veterinary medicine (Slaska and Grzybowska-Szatkowska 2011;Nisztuk-Pacek 2016;Surdyka and Slaska 2016a, b).…”

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confidence: 99%

Two mitochondrial genes are associated with performance traits in farmed raccoon dogs (Nyctereutes procyonoides)

Nisztuk-Pacek¹,

Ślaska²,

Zięba³

et al. 2018

CZECH J ANIM SCI

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The relationships between chosen mitochondrial genes polymorphisms and performance traits in raccoon dogs were determined. The study involved 354 farmed raccoon dogs. Blood collected from the animals was the analysed biological material. Mitochondrial DNA genes, i.e. MT-CO1 (mitochondrially encoded cytochrome c oxidase I), MT-CO2 (mitochondrially encoded cytochrome c oxidase II), and MT-CYB (mitochondrially encoded cytochrome b) were amplified using the polymerase chain reaction method. The amplicons obtained were sequenced and subjected to bioinformatics analysis. Based on the nucleotide sequences, three haplotypes for the MT-CO1 gene fragment and two haplotypes for the MT-CO2 gene fragment were identified. The sequence of the MT-CYB gene was monomorphic. Based on the haplotypes, five previously undescribed mitochondrial haplogroups were determined. Statistical analysis revealed significant differences between the values of three of the five investigated performance traits of raccoon dogs and the identified haplotypes and mitochondrial haplogroups, taking into account predictors of direct additive effects, additive maternal effects, and fixed specific maternal environmental effects. The new mitochondrial haplogroups identified in the farmed raccoon dog population may imply constant emergence of adaptive mutations that are conserved in subsequent generations. The results of the association study indicate a statistically significant association between haplotypes and mitochondrial haplogroups of farmed raccoon dogs and their body weight, body size, and colour type, which allows considering MT-CO1 and MT-CO2 genes as candidate genes encoding these traits in raccoon dogs. The results of the molecular analyses can be applied to improve the performance traits in farmed raccoon dogs.

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Paternal Leakage of Mitochondrial DNA in the Raccoon Dog (Nyctereutes Procyonoides Gray 1834)

Nisztuk-Pacek

Ślaska

Grzybowska‐Szatkowska

et al. 2019

Annals of Animal Science

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the aim of the study was to describe the mechanism of mitochondrial dna inheritance in a group of farmed raccoon dogs. the study involved 354 individuals. Whole peripheral blood was the research material. dna was isolated and Pcr was performed for two fragments of mitochondrial genes: coX1 (cytochrome oxidase subunit 1 gene) and coX2 (cytochrome oxidase subunit 2 gene). the Pcr products were sequenced and subjected to bioinformatics analyses. three mitochondrial haplotypes were identified in the coX1 gene fragment and two in the coX2 gene fragment. the analysis of mtDNA inheritance in the paternal line confirmed the three cases of paternal mtDNA inheritance, i.e. the so-called "paternal leakage" in the analysed population. in two families, all offspring inherited paternal mitochondrial dna, whereas in one family one descendant inherited paternal mtdna and another one inherited maternal mtdna. the lineage data indicated that one female which inherited maternal mitochondrial dna transferred it onto the next generation. To sum up, the results of the study for the first time demonstrated the phenomenon of "paternal leakage" in farmed raccoon dogs, which facilitated description of mitochondrial dna inheritance in the paternal line.

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Genetic variability of farmed fur animals of the Canidae family assessed by nuclear and mitochondrial DNA analysis

Cited by 3 publications

References 13 publications

Assessment of chosen semen characteristics of two colour morphs of the Arctic fox Alopex lagopus L.

Assessment of chosen semen characteristics of two colour morphs of the Arctic fox Alopex lagopus L.

Two mitochondrial genes are associated with performance traits in farmed raccoon dogs (Nyctereutes procyonoides)

Paternal Leakage of Mitochondrial DNA in the Raccoon Dog (Nyctereutes Procyonoides Gray 1834)

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