Genetic variability of &lt;i&gt;Papaya ringspot virus&lt;/i&gt; isolates in Norte de Santander - Colombia

Chaves-Bedoya, Giovanni; Ortiz-Rojas, Luz Yineth

doi:10.15446/agron.colomb.v33n2.50095

Cited by 9 publications

(6 citation statements)

References 41 publications

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“…Su genoma es de ARN monocatenario de polaridad positiva, de alrededor de 10,000 nucleótidos de longitud y cuenta con una cola poli(A) en el extremo 3' (Kumar et al, 2019). La región central del genoma del PRSV-p es altamente conservada, mientras que la región Nterminal es más variable (Ortiz-Rojas, 2015). La acumulación de mutaciones, los procesos de selección natural y el movimiento a larga distancia en estructuras de las plantas donde los plasmodesmos son limitados, son factores que incrementan la diversidad genética (Chaves-Bedoya et al, 2015).…”

Section: Introductionunclassified

“…La región central del genoma del PRSV-p es altamente conservada, mientras que la región Nterminal es más variable (Ortiz-Rojas, 2015). La acumulación de mutaciones, los procesos de selección natural y el movimiento a larga distancia en estructuras de las plantas donde los plasmodesmos son limitados, son factores que incrementan la diversidad genética (Chaves-Bedoya et al, 2015). Es importante señalar que los potyvirus se han utilizado como herramienta para explicar cómo las especies de virus en plantas no son una población uniforme y acumulan una gran cantidad de variación fenotípica y genética.…”

Section: Introductionunclassified

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VARIABILIDAD GENÉTICA DE AISLADOS DE PRSV-p DE PAPAYO EN LA ZONA CENTRO DEL ESTADO DE VERACRUZ, MÉXIC

Sósol-Reyes

Villanueva-Jiménez

Osorio-Acosta

et al. 2022

Trop Subtrop Agroecosyst

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Background: The Potyvirus Papaya ringspot virus type P (PRSV-p) causes the papaya (Carica papaya L.) ringspot disease, which causes damage to the plantations of this crop. In regions where there is movement of plant material and continuous production processes, there is greater genetic variability of this virus; such is the case of the State of Veracruz, Mexico, one of the main papaya producers in the country. Objective: To analyze the genetic variability of isolates of PRSV-p, present in commercial papaya plantations in the central zone of the State of Veracruz. Methodology: Leaves with differential symptoms of the disease were collected. The capsid protein (CP) coding region was amplified by RT-PCR to confirm the identity of the virus. The sequences obtained were analyzed, aligned and a phylogenetic tree was obtained. Results: The presence of PRSV-p was confirmed by RT-PCR. Through sequence analysis, high diversity was observed in the first 50 amino acids encoded in the N-terminal region of CP. In the phylogenetic tree, three viral genetic groups were obtained. The largest conserved sites of interest (even the WCIEN box and QMKAAA) were found near the C-terminal region of the CP. 100% homology was observed in the central and C-terminal region of the Ver75Co isolate, with respect to the consensus sequence. Implications: The results show the genetic diversity of the virus in the central zone of the state of Veracruz, which will allow genetic modifications to improve resistance in papaya plants. Conclusions: There is genetic variability of PRSV-p in Veracruz, there are conserved sites of interest (WCIEN and QMKAAA) and changes in the N-terminal region, which includes the EK repetitive region and the DAG codon. These changes may be a genetic characteristic of the virus for adaptation. The Ver75Co isolate may be the point of change that gives rise to the other isolates of this work. The phylogenetic tree shows a single genetic group of the virus, which is distributed in three different clades.

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Section: Introductionunclassified

VARIABILIDAD GENÉTICA DE AISLADOS DE PRSV-p DE PAPAYO EN LA ZONA CENTRO DEL ESTADO DE VERACRUZ, MÉXIC

Sósol-Reyes

Villanueva-Jiménez

Osorio-Acosta

et al. 2022

Trop Subtrop Agroecosyst

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“…In Colombia, this potyvirus has been reported in crops such as the tree tomato (Ayala et al, 2010), papaya (Chaves-Bedoya andOrtiz-Rojas, 2015;Ortiz-Rojas and Chaves-Bedoya, 2017), potato (Riascos et al, 2017) and recently pepper (Rivera-Toro et al, 2021). Breeding programs need to know which viral agents, such as potyviruses, are present in the field.…”

Section: Introductionmentioning

confidence: 99%

Presence of Potyvirus in the Norte de Santander department and molecular characterization of NIb protein in Colombian SCMV isolates

Chaves-Bedoya

Ortiz-Rojas

2021

Rev. Colomb. Cienc. Hortic.

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Potyviruses are the largest genus of plant viruses that cause significant losses over a wide range of crops. In this paper, the presence of potyvirus in different plant crops in the provinces of Ocaña and Pamplona located in the north and south of the Department of Norte de Santander (Colombia) was evaluated with RT-PCR analysis using universal oligonucleotides specific to the region that encodes the NIB protein. The results indicate the presence of several potyvirus in Pamplona in economically important crops such as corn (Zea mays), tomato (Solanum lycopersicum), potato (Solanum tuberosum) and zucchini (Cucurbita pepo). In Ocaña, potyvirus was found in bean (Phaseolus vulgaris), corn and pumpkin (Cucurbita maxima). In corn, one of the most important crops, the presence of the Sugarcane mosaic virus (SCMV) was confirmed with nucleotide sequencing. This is the first report of this virus in the department. The presence of several potyviruses in different crops in Norte de Santander indicate an alarming phytosanitary condition that must be addressed with priority to establish detection and control systems that maximize production, ensure agricultural sustainability, and propose certification schemes and improvement programs to reduce economic losses.

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“…In addition, a short polypeptide (PIPO) is expressed within the P3 cistron by frame shifting (Chung et al, 2008). Among the 11 encoded functional proteins, the coat protein (CP) sequence has been frequently used in strain identification, species classification, and phylogenetic analysis of potyviruses (Chaves‐Bedoya & Ortiz‐Rojas, 2015; Cuevas et al, 2012; Gao et al, 2016; Gibbs & Ohshima, 2010; Martinez, 2014; Wu et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity analysis of papaya leaf distortion mosaic virus isolates infecting transgenic papaya “Huanong No. 1” in South China

Xie

et al. 2020

Ecology and Evolution

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The commercialized genetically modified papaya “Huanong No. 1” has been utilized to successfully control the destructive virus‐papaya ringspot virus (PRSV) in South China since 2006. However, another new emerging virus, papaya leaf distortion mosaic virus (PLDMV), was found in some PRSV‐resistant transgenic plants in Guangdong and Hainan provinces of South China through a field investigation from 2012 to 2019. The survey results showed that “Huanong No. 1” papaya plants are susceptible to PLDMV, and the disease prevalence in Hainan Province is generally higher than that in Guangdong Province. Twenty representative isolates were selected to inoculate “Huanong No. 1,” and all of the inoculated plants showed obvious disease symptoms similar to those in the field, indicating that PLDMV is a new threat to widely cultivated transgenic papaya in South China. Phylogenetic analysis of 111 PLDMV isolates in Guangdong and Hainan based on the coat protein nucleotide sequences showed that PLDMV isolates can be divided into two groups. The Japan and Taiwan China isolates belong to group I, whereas the Guangdong and Hainan isolates belong to group II and can be further divided into two subgroups. The Guangdong and Hainan isolates are far different from the Japan and Taiwan China isolates and belong to a new lineage. Further analysis showed that the Guangdong and Hainan isolates had a high degree of genetic differentiation, and no recombination was found. These isolates deviated from neutral evolution and experienced population expansion events in the past, which might still be unstable. The results of this study provide a theoretical basis for clarifying the evolutionary mechanism and population genetics of the virus and for preventing and controlling the viral disease.

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Genetic variability of <i>Papaya ringspot virus</i> isolates in Norte de Santander - Colombia

Cited by 9 publications

References 41 publications

VARIABILIDAD GENÉTICA DE AISLADOS DE PRSV-p DE PAPAYO EN LA ZONA CENTRO DEL ESTADO DE VERACRUZ, MÉXIC

VARIABILIDAD GENÉTICA DE AISLADOS DE PRSV-p DE PAPAYO EN LA ZONA CENTRO DEL ESTADO DE VERACRUZ, MÉXIC

Presence of Potyvirus in the Norte de Santander department and molecular characterization of NIb protein in Colombian SCMV isolates

Genetic diversity analysis of papaya leaf distortion mosaic virus isolates infecting transgenic papaya “Huanong No. 1” in South China

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