Genetic Variability of NS5B Region of Hepatitis C Virus in Togo

Abstract

Background: Hepatitis C virus (HCV) infection is a major cause of chronic liver disease worldwide. The high prevalence and spread rates of the disease require serious actions to stop these rates. Determination of HCV genotypes and subgenotypes adds significant knowledge about the epidemiology of the disease, and provides and added value in the decision-making process of what strategy to follow and what therapy response to expert. The molecular epidemiology and genetic variability of HCV variant circulating in Togo still need further analysis. Materials and methods: The HCV molecular epidemiology in Togo was studies using direct sequencing and further phylogenetic analysis of a partial NS5B region of the HCV from 56 patients. HCV genotype and subtype were successfully determined in 41 out of 53. Results: The highest prevalent genotype was genotype 2, which was found in 30 patients (73,2%) followed by genotype 1 that was isolated from 7 patients (17,1%), genotype 4 was isolated from 3 patients (7,3%) and 1 patient (2,4%) was infected by genotype 6. Conclusion: The molecular epidemiology study revealed high prevalence of genotype 2 among Togolese patients followed by genotype 1. HCV RNA Extraction and HCV-NS5B amplification.HCV RNA extraction was carried out by using QIAsymphony DPS virus/Pathogen Midi Kit on automate

Hepatitis C: A Review on Current and Emerging Genotyping Assays

“…Another study revealed that DNA sequencing on NS5B region was able to precisely detect few HCV subtypes; 1a, 1b, 2a, 3b, 6a, as well as new subtypes; 1b-2a, 1b-2k and 6d-6k (42). NS5B region was successfully sequenced in around 77% of the positive samples reported in the recent study by Anyovi et al (43). Therefore, NS5B region is among the recommended region for HCV genotyping as well as Core due to the highly informative in phylogeny analysis and identification of HCV strains (43).…”

“…NS5B region was successfully sequenced in around 77% of the positive samples reported in the recent study by Anyovi et al (43). Therefore, NS5B region is among the recommended region for HCV genotyping as well as Core due to the highly informative in phylogeny analysis and identification of HCV strains (43).…”