Genetic variability of pig and human rotavirus group A isolates from Slovakia

Salamunova, Slavomira; Jacková, Anna; Csank, Tomáš; Mandelik, Rene; Novotný, Jaroslav; Beckova, Z; Helmova, L; Vilček, Štefan

doi:10.1007/s00705-019-04504-6

Cited by 5 publications

(1 citation statement)

References 18 publications

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“…According to the literature data, double intergroup G3P[8] reassortants, which in a number of articles are also called "equine-like" RVAs, dominated in Thailand (2014-16), Indonesia (2013-16), Malaysia (2018-19), Japan (2017-18), Brazil (2018-19), Russia (2019) [33][34][35][36][37][38]. In addition, G3P[8] reassortants were found in the territories of European countries: Spain (2014-15), Italy (2017-19), Slovakia (2019), Germany (2016), Hungary (2015), Turkey (2016-17), as well as China (2017-18) and Kenya (2018) [39][40][41][42][43][44][45][46].…”

Section: Discussionmentioning

confidence: 99%

Phylogeographic characteristics of reassortant DS-1-like G3P[8]-strains of rotavirus type A isolated in Nizhny Novgorod (Russia).

Морозова

Сашина

Епифанова

et al. 2023

Preprint

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Since 2013, there has been an increase in reports of the spread of a double intergroup reassortant strain of rotavirus type A (RVA) with the genotype G3P[8] and other genes belonging to the second genogroup I2-R2-C2-M2-A2-N2-T2-E2-H2. Our study found an increase in the proportion of G3P[8] with a short migration profile of genome segments in RNA-PAGE from 15% (2020-2021) to 53% (2021-2022) in Nizhny Novgorod (Russia). The VP7, VP4 and VP6 genes were sequenced by the Sanger method. It was found that the studied strains belong to the genotype G3P[8]-I2, and are reassortants between Wa- and DS-1-like rotaviruses. Phylogenetic analysis revealed that VP7 sequences of studied strains are closely related to DS-1-like G3P[8] RVA, which have been isolated in other countries. The RVAs isolated in Nizhny Novgorod carried the allele P[8]-3.1 of the VP4 gene, as well as other worldwide spreading G3P[8] DS-1-like reassortants. None of the VP6 sequences of studied G3P[8] RVA were included in the group with equine-like strains isolated in other countries. Most VP6 alleles of Nizhny Novgorod G3P[8] DS-1-like RVAs clustered with G2P[4], which were studied earlier. This fact may indicate a local reassortment of the spread strains.

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Section: Discussionmentioning

confidence: 99%

Phylogeographic characteristics of reassortant DS-1-like G3P[8]-strains of rotavirus type A isolated in Nizhny Novgorod (Russia).

Морозова

Сашина

Епифанова

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

Phylodynamic characteristics of reassortant DS-1-like G3P[8]-strains of rotavirus type A isolated in Nizhny Novgorod (Russia)

Morozova,

Sashina,

Epifanova

et al. 2023

Braz J Microbiol

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Genome analyses of species A rotavirus isolated from various mammalian hosts in Northern Ireland during 2013−2016

Lagan,

Mooney,

Lemon

2023

Virus Evolution

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Rotavirus group A (RVA) is the most important cause of acute diarrhoea and severe dehydration in young mammals. Infection in livestock is associated with significant mortality and economic losses and, together with wildlife reservoirs, acts as a potential source of zoonotic transmission. Therefore, molecular surveillance of circulating RVA strains in animal species is necessary to assess the risks posed to humans and their livestock. An RVA molecular epidemiological surveillance study on clinically diseased livestock species revealed high prevalence in cattle and pigs (31 per cent and 18 per cent, respectively) with significant phylogenetic diversity including a novel and divergent ovine artiodactyl DS-1-like constellation G10-P[15]-I2-R2-C2-M2-A11-N2-T6-E2-H3. An RVA gene reassortment occurred in an RVA asymptomatic pig and identified as a G5-P[13] strain, and a non-structural protein (NSP)2 gene had intergenomically reassorted with a human RVA strain (reverse zoonosis) and possessed a novel NSP4 enterotoxin E9 which may relate to the asymptomatic RVA infection. Analysis of a novel sheep G10-P[15] strain viral protein 4 gene imparts a putative homologous intergenic and interspecies recombination event, subsequently creating the new P[15] divergent lineage. While surveillance across a wider range of wildlife and exotic species identified generally negative or low prevalence, a novel RVA interspecies transmission in a non-indigenous pudu deer (zoo origin) with the constellation of G6-P[11]12-R2-C2-M2-A3-N2-T6-E2-H3 was detected at a viral load of 11.1 log10 copies/gram. The detection of novel emerging strains, interspecies reassortment, interspecies infection, and recombination of RVA circulating in animal livestock and wildlife reservoirs is of paramount importance to the RVA epidemiology and evolution for the One Health approach and post-human vaccine introduction era where highly virulent animal RVA genotypes have the potential to be zoonotically transmitted.

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Genetic variability of pig and human rotavirus group A isolates from Slovakia

Cited by 5 publications

References 18 publications

Phylogeographic characteristics of reassortant DS-1-like G3P[8]-strains of rotavirus type A isolated in Nizhny Novgorod (Russia).

Phylogeographic characteristics of reassortant DS-1-like G3P[8]-strains of rotavirus type A isolated in Nizhny Novgorod (Russia).

Phylodynamic characteristics of reassortant DS-1-like G3P[8]-strains of rotavirus type A isolated in Nizhny Novgorod (Russia)

Genome analyses of species A rotavirus isolated from various mammalian hosts in Northern Ireland during 2013−2016

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