Genetic variability of Rickettsia spp. in Ixodes persulcatus ticks from continental and island areas of the Russian Far East

Igolkina, Yana; Бондаренко, Е. И.; Рар, В. А.; Epikhina, Tamara; Высочина, Н. П.; Pukhovskaya, N. M.; Tikunov, Artem; Иванов, Л. И.; Golovljova, Irina; Ivanov, M. Ja.; Tikunova, Nina

doi:10.1016/j.ttbdis.2016.06.005

Cited by 16 publications

(25 citation statements)

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“…Rickettsia helvetica, a tick-borne rickettsiae species, is also frequently detected in Europe and Asia [2,28,29], being reported to be the prevalent Rickettsia species in some of the regions, e.g. in Germany [30], Slovakia [31] and Sakhalin Island in Russia [29]. Estonia belongs to one of the predominant region as well as over 95% of all Rickettsia species detected in questing [12] and rodent-attached ticks in the present study were R. helvetica.…”

Section: Discussionsupporting

confidence: 55%

“…Serological or molecular tools have been used to detect R. helvetica infection in samples from patients with suspected Lyme neuroborreliosis in the Netherlands [10], with unexplained fever following a tick bite in France and Italy [11] and with rash, febrile illness and meningitis in Sweden [8,9]. Rickettsia helvetica, a tick-borne rickettsiae species, is also frequently detected in Europe and Asia [2,28,29], being reported to be the prevalent Rickettsia species in some of the regions, e.g. in Germany [30], Slovakia [31] and Sakhalin Island in Russia [29].…”

Section: Discussionmentioning

confidence: 99%

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Rickettsia spp. in rodent-attached ticks in Estonia and first evidence of spotted fever group Rickettsia species Candidatus Rickettsia uralica in Europe.

Vikentjeva

Geller

Remm

et al. 2021

Preprint

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BACKGROUND: Rickettsia spp. are human pathogens that cause a number of diseases and are transmitted by arthropods, such as ixodid ticks. Estonia is one of few regions where the distribution area of two medically important tick species, Ixodes persulcatus and I. ricinus, overlaps. The presence of the nidicolous rodent-associated I. trianguliceps has also recently been shown in Estonia. Although there is no data available in Estonia on human disease caused by tick-borne Rickettsia spp., the presence of three Rickettsia species in non-nidicolous ticks was also previously reported. The aim of this study was to detect, identify and partially characterize Rickettsia species in nidicolous and non-nidicolous ticks attached to rodents.RESULTS: Larvae and nymphs of I. ricinus (n = 1004), I. persulcatus (n = 75) and I. trianguliceps (n = 117) removed from rodents and shrews caught in different parts of Estonia were studied for the presence of Rickettsia spp. by nested PCR. Ticks were collected from 314 small animals of 5 species (Myodes glareolus (bank voles), Apodemus flavicollis (yellow necked mice), A. agrarius (striped field mice), Microtus subterranius (pine voles) and Sorex araneus (common shrews)). Rickettsial DNA was detected in 8.7% (103/1186) of the studied ticks. In addition to R. helvetica previously found in questing ticks, this study reports the first identification of the recently described I. trianguliceps-associated Candidatus R. uralica west of the Ural Mountains.

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Section: Discussionsupporting

confidence: 55%

Section: Discussionmentioning

confidence: 99%

Rickettsia spp. in rodent-attached ticks in Estonia and first evidence of spotted fever group Rickettsia species Candidatus Rickettsia uralica in Europe.

Vikentjeva

Geller

Remm

et al. 2021

Preprint

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“…R. tarasevichiae” is known to be a predominant Rickettsia species and was identified in 45–90% of individuals collected in most of the examined regions. R. heilongjiangensis , R. helvetica , R. raoultii and R. sibirica are substantially rarer species of Rickettsia [15, 40, 49, 50]. Surprisingly, I. pavlovskyi ticks were significantly less often infected by “ Ca .…”

Section: Discussionmentioning

confidence: 99%

Detection and genetic characterization of a wide range of infectious agents in Ixodes pavlovskyi ticks in Western Siberia, Russia

et al. 2017

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BackgroundThe Ixodes pavlovskyi tick species, a member of the I. persulcatus/I. ricinus group, was discovered in the middle of the 20th century in the Russian Far East. Limited data have been reported on the detection of infectious agents in this tick species. The aim of this study was to investigate the prevalence and genetic variability of a wide range of infectious agents in I. pavlovskyi ticks collected in their traditional and recently invaded habitats, the Altai Mountains and Novosibirsk Province, respectively, which are both located within the Western Siberian part of the I. pavlovskyi distribution area.ResultsThis study reports the novel discovery of Borrelia bavariensis, Rickettsia helvetica, R. heilongjiangensis, R. raoultii, “Candidatus Rickettsia tarasevichiae”, Anaplasma phagocytophilum, Ehrlichia muris, “Candidatus Neoehrlichia mikurensis” and Babesia microti in I. pavlovskyi ticks. In addition, we confirmed the previous identification of B. afzelii, B. garinii and B. miyamotoi, as well as tick-borne encephalitis and Kemerovo viruses in this tick species. The prevalence and some genetic characteristics of all of the tested agents were compared with those found in I. persulcatus ticks that were collected at the same time in the same locations, where these tick species occur in sympatry. It was shown that the prevalence and genotypes of many of the identified pathogens did not significantly differ between I. pavlovskyi and I. persulcatus ticks. However, I. pavlovskyi ticks were significantly more often infected by B. garinii and less often by B. bavariensis, B. afzelii, “Ca. R. tarasevichiae”, and E. muris than I. persulcatus ticks in both studied regions. Moreover, new genetic variants of B. burgdorferi (sensu lato) and Rickettsia spp. as well as tick-borne encephalitis and Kemerovo viruses were found in both I. pavlovskyi and I. persulcatus ticks.ConclusionAlmost all pathogens that were previously detected in I. persulcatus ticks were identified in I. pavlovskyi ticks; however, the distribution of species belonging to the B. burgdorferi (sensu lato) complex, the genus Rickettsia, and the family Anaplasmataceae was different between the two tick species. Several new genetic variants of viral and bacterial agents were identified in I. pavlovskyi and I. persulcatus ticks.

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“…Ixodes persulcatus, a tick species distributed widely from the eastern Baltic coast to Japan (22)(23)(24)(25), has been reported to harbour symbionts including the Montezuma agent, now called Candidatus Lariskella arthropodarum (26)(27)(28), Coxiella and Spiroplasma spp. (29), as well as human and livestock pathogens including tick-borne encephalitis virus (TBEV), Kemerovo virus, Alongshan virus, Anaplasma phagocytophilum, Candidatus Neoehrlichia mikurensis, Ehrlichia muris, Rickettsia helvetica, Rickettsia heilongjiangensis, Candidatus Rickettsia tarasevichiae, Borrelia miyamotoi, Borrelia burgdorferi sensu lato, Theileria equi and several species of Babesia (25,(30)(31)(32)(33)(34)(35)(36)(37)(38). Co-infections with multiple pathogens and symbionts are common (29,35,39).…”

Section: Introductionmentioning

confidence: 99%

Spiroplasma Isolated From Third-Generation Laboratory Colony Ixodes persulcatus Ticks

et al. 2021

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Spiroplasma are vertically-transmitted endosymbionts of ticks and other arthropods. Field-collected Ixodes persulcatus have been reported to harbour Spiroplasma, but nothing is known about their persistence during laboratory colonisation of this tick species. We successfully isolated Spiroplasma from internal organs of 6/10 unfed adult ticks, belonging to the third generation of an I. persulcatus laboratory colony, into tick cell culture. We screened a further 51 adult male and female ticks from the same colony for presence of Spiroplasma by genus-specific PCR amplification of fragments of the 16S rRNA and rpoB genes; 100% of these ticks were infected and the 16S rRNA sequence showed 99.8% similarity to that of a previously-published Spiroplasma isolated from field-collected I. persulcatus. Our study shows that Spiroplasma endosymbionts persist at high prevalence in colonised I. persulcatus through at least three generations, and confirms the usefulness of tick cell lines for isolation and cultivation of this bacterium.

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Genetic variability of Rickettsia spp. in Ixodes persulcatus ticks from continental and island areas of the Russian Far East

Cited by 16 publications

References 23 publications

Rickettsia spp. in rodent-attached ticks in Estonia and first evidence of spotted fever group Rickettsia species Candidatus Rickettsia uralica in Europe.

Rickettsia spp. in rodent-attached ticks in Estonia and first evidence of spotted fever group Rickettsia species Candidatus Rickettsia uralica in Europe.

Detection and genetic characterization of a wide range of infectious agents in Ixodes pavlovskyi ticks in Western Siberia, Russia

Spiroplasma Isolated From Third-Generation Laboratory Colony Ixodes persulcatus Ticks

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