Genetic Variability Trend of Lusitano Horse Breed Reared in Italy

Cozzi, M.C.; Valiati, P.; Longeri, M.; Ferreira, Carlos; Ferreira, Sofia Abreu

doi:10.3390/ani12010098

Cited by 6 publications

(3 citation statements)

References 28 publications

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“…Recently, many studies have been focused on the loss of biodiversity [ 23 , 24 , 25 ] or the increase in deleterious genotypes [ 26 ] caused by such inbreeding (as reviewed in [ 7 ]). In the last 200 years, the inbreeding practice led to the phenotypical expression at homozygous sites of deleterious variants [ 27 ], as highlighted when the coefficients are calculated in order to evaluate the genetic diversity among different horse breeds [ 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 , 54 , 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 , 64 , 65 , 66 , 67 , 68 , 69 , 70 , 71 , 72 , 73 , 74 , 75 ,…”

Section: Introductionmentioning

confidence: 99%

Unlocking Horse Y Chromosome Diversity

Cardinali

Giontella

Tommasi

et al. 2022

Genes

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The present equine genetic variation mirrors the deep influence of intensive breeding programs during the last 200 years. Here, we provide a comprehensive current state of knowledge on the trends and prospects on the variation in the equine male-specific region of the Y chromosome (MSY), which was assembled for the first time in 2018. In comparison with the other 12 mammalian species, horses are now the most represented, with 56 documented MSY genes. However, in contrast to the high variability in mitochondrial DNA observed in many horse breeds from different geographic areas, modern horse populations demonstrate extremely low genetic Y-chromosome diversity. The selective pressures employed by breeders using pedigree data (which are not always error-free) as a predictive tool represent the main cause of this lack of variation in the Y-chromosome. Nevertheless, the detailed phylogenies obtained by recent fine-scaled Y-chromosomal genotyping in many horse breeds worldwide have contributed to addressing the genealogical, forensic, and population questions leading to the reappraisal of the Y-chromosome as a powerful genetic marker to avoid the loss of biodiversity as a result of selective breeding practices, and to better understand the historical development of horse breeds.

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Section: Introductionmentioning

confidence: 99%

Unlocking Horse Y Chromosome Diversity

Cardinali

Giontella

Tommasi

et al. 2022

Genes

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“…The SEA sire line revealed the lowest value of genetic diversity compared with the other populations (Table 1). In general, the founder effect, genetic drift, selection, and/or inbreeding result in low genetic variability in farmed stocks, including horses [61,62]. The level of genetic diversity found in a population largely depends on the mating system, the evolutionary history of a horse breed, and the population history, as well as the geographical ranges of the populations [63].…”

Section: Genetic Diversity Within and Among The Arabian Horse Sire Linesmentioning

confidence: 99%

Genetic Diversity and Population Structure among Arabian Horse Genealogical Lineages in Bulgaria

Hristov,

Radoslavov,

Mehandjyiski

et al. 2024

Diversity

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The present research aimed to characterize the genetic diversity and relationships among extant Arabian horse sire lines in Bulgaria, using 15 equine microsatellite markers. The evaluation included 537 Arabian horses representing nine sire lines (SAKLAWI I, LATIF, SEANDERICH, IBRAHIM, SHABAB, DJEBEL MOUSA, KUHAILAN AFAS, BAIRACTAR, and SARHAN). The obtained results indicated that within these lines, the mean number of alleles ranged from 4.15 in SARHAN to 5.54 in SAKLAWI I and LATIF. The mean expected heterozygosity (He) ranged from 0.54 in the SEANDERICH line to 0.67 in SAKLAWI I. The inbreeding coefficient for the entire Arabian populations was rather low: FIS = −0.109, fluctuating from −0.204 in SHABAB to −0.041 in SAKLAWI I. The mean genetic differentiation, FST, was 0.096, demonstrating that nearly 90% of the total genetic variation was due to genetic differentiation within each population. STRUCTURE analysis indicated a genetic similarity between SHABAB and LATIF, between IBRAHIM and KUHAILAN AFAS, as well as between SAKLAWI I, SEANDERICH, and BAIRACTAR. This study of the genetic diversity of Arabian sire lines in Bulgaria can assist in developing a national strategy for the exclusion of non-purebred animals from breeding programs in order to preserve the genetic profile of the original Arabian lines.

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“…Genotyping of microsatellite DNA polymorphisms was carried out through agarose gel electrophoresis (Asif et al 2008), then DNA were analyzed using GeneScan and Genotyper® software (Cozzi et al 2022). Genetic diversity measures include allele frequency, the observed number of alleles, banding patterns and expected heterozygosity, molecular variance, and similarity relationship based on the dendrogram.…”

Section: Microsatellite Analysismentioning

confidence: 99%

Revealing the genetic diversity of Sumbawa endemic horse using microsatellite-based DNA fingerprint

SUKRI¹,

DEWI²,

PRIMAWATI³

et al. 2022

Biodiversitas

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Abstract. Sukri A, Dewi IN, Primawati SB, Wangiyana IGAS, Muttaqin Z, Winaya A. 2022. Revealing the genetic diversity of Sumbawa endemic horse using microsatellite-based DNA fingerprint. Biodiversitas 23: 4153-4159. Sumbawa horse (Equus caballus) is a genetic resource that must be preserved. Sumbawa horses have a significant economic, social, and cultural role. Information on the genetic status of the Sumbawa horse is needed as a step in developing a long-term conservation strategy. Therefore, this study aims to reveal the genetic diversity of the Sumbawa endemic horse using a microsatellite-based DNA fingerprint. DNA isolation was conducted from horse blood samples. Blood samples were taken from two horse farms with 24 individual horses from two different populations, namely Lalar and Liang,West Sumbawa District, Indonesia. Blood was taken from the jugular vein and collected by a qualified veterinarian from the Faculty of Veterinary Medicine, Universitas Pendidikan Mandalika. A total of 4 microsatellite primers were used in this study, INRA032, HEL09, CA425, and AHT4. This study revealed that the genetic diversity of horses in the Lalar population was higher than Liang. A greater number of alleles reinforces this; higher number and frequency of bands; and the presence of specific bands that indicate unique alleles. This research shows that the Sumbawa horse is unique from other horse breeds in the world. This is evidenced by the lower number of alleles per locus (Na) with a maximum number of two alleles per locus. Sumbawa horses have higher observed heterozygosity (Ho) than expected heterozygosity (He), with a Ho value less than 0.5. Analysis of Molecular Variance result has shown that variation within the population was higher than among the population. This is presumably due to the high gene flow in both horse populations caused by inbreeding. In general, AHT4 primers had the best ability to reveal the genetic diversity of Sumbawa horses with the highest Shannon's information index compared to other markers.

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Genetic Variability Trend of Lusitano Horse Breed Reared in Italy

Cited by 6 publications

References 28 publications

Unlocking Horse Y Chromosome Diversity

Unlocking Horse Y Chromosome Diversity

Genetic Diversity and Population Structure among Arabian Horse Genealogical Lineages in Bulgaria

Revealing the genetic diversity of Sumbawa endemic horse using microsatellite-based DNA fingerprint

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