Genetic variants of p21 and p27 and hepatocellular cancer risk in a Chinese Han population: A case‐control study

Liu, Fei; Yue, Wei; Luo, Limei; Wang, Wentao; Yan, Lvnan; Wen, Tianfu; Xu, Mingqing; Yang, Jiayin; Li, Bo

doi:10.1002/ijc.27885

Cited by 23 publications

(15 citation statements)

References 52 publications

(111 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The G allele of the CDKN1B gene (rs2066827) has been associated with an increased risk for thyroid (13), squamous cell (28), prostate, and breast cancers (27,30). However, whether this polymorphism is associated with a better or worse prognosis remains uncertain (13,21,28,31). Pasqualini et al also found differences in the frequency of the WT (TT -53.6%) and polymorphic alleles Figure 1 Graphical representation of the relative contribution of the CDKN1B and CDKN2A genes to sporadic medullary thyroid carcinoma risk according to a stepwise regression analysis.…”

Section: Discussionmentioning

confidence: 99%

“…Nine polymorphisms were genotyped: CDKN1A (rs1801270 and rs1059234), CDKN1B (rs2066827 and rs34330), CDKN2A (rs11515), CDKN2B (rs2069426, rs3731239, and rs1063192), and CDKN2C (rs12885) using the TaqMan system (Applied Biosystems), as described previously (17). These SNPs were chosen because they have previously been associated with thyroid carcinoma or other tumor risks as described in Table 1 (13,15,16,20,21,22).…”

Section: Genotypingmentioning

confidence: 99%

See 1 more Smart Citation

Polymorphisms of cell cycle control genes influence the development of sporadic medullary thyroid carcinoma

Barbieri

Bufalo

Secolin

et al. 2014

European Journal of Endocrinology

View full text Add to dashboard Cite

Background: The role of key cell cycle regulation genes such as, CDKN1B, CDKN2A, CDKN2B, and CDKN2C in sporadic medullary thyroid carcinoma (s-MTC) is still largely unknown. Methods: In order to evaluate the influence of inherited polymorphisms of these genes on the pathogenesis of s-MTC, we used TaqMan SNP genotyping to examine 45 s-MTC patients carefully matched with 98 controls. Results: A multivariate logistic regression analysis demonstrated that CDKN1B and CDKN2A genes were related to s-MTC susceptibility. The rs2066827*GTCGG CDKN1B genotype was more frequent in s-MTC patients (62.22%) than in controls (40.21%), increasing the susceptibility to s-MTC (ORZ2.47; 95% CIZ1.048-5.833; PZ0.038). By contrast, the rs11515*CGCGG of CDKN2A gene was more frequent in the controls (32.65%) than in patients (15.56%), reducing the risk for s-MTC (ORZ0.174; 95% CIZ0.048-0.627; PZ0.0075). A stepwise regression analysis indicated that two genotypes together could explain 11% of the total s-MTC risk. In addition, a relationship was found between disease progression and the presence of alterations in the CDKN1A (rs1801270), CDKN2C (rs12885), and CDKN2B (rs1063192) genes. WTrs1801270 CDKN1A patients presented extrathyroidal tumor extension more frequently (92%) than polymorphic CDKN1A rs1801270 patients (50%; PZ0.0376). Patients with the WT CDKN2C gene (rs12885) presented larger tumors (2.9G1.8 cm) than polymorphic patients (1.5G0.7 cm; PZ0.0324). On the other hand, patients with the polymorphic CDKN2B gene (rs1063192) presented distant metastases (36.3%; PZ0.0261). Conclusion: In summary, we demonstrated that CDKN1B and CDKN2A genes are associated with susceptibility, whereas the inherited genetic profile of CDKN1A, CDKN2B, and CDKN2C is associated with aggressive features of tumors. This study suggests that profiling cell cycle genes may help define the risk and characterize s-MTC aggressiveness.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Genotypingmentioning

confidence: 99%

Polymorphisms of cell cycle control genes influence the development of sporadic medullary thyroid carcinoma

Barbieri

Bufalo

Secolin

et al. 2014

European Journal of Endocrinology

View full text Add to dashboard Cite

show abstract

“…Based on the search strategy, 1,641 records were retrieved. In this meta-analysis, ten studies15161718192021222324 involving 11,214 cases and more than 8,776 controls were identified from the electronic databases according to the inclusion criteria. Characteristics of the identified studies are presented in Table 1.…”

Section: Resultsmentioning

confidence: 99%

“…The sample size in these studies ranged from 143 to 9,030 individuals. The genotype distributions of cases and controls were presented in seven studies15161718202324. Other three studies192122 only reported the ORs with 95% CIs in more than two of genetic models, such as homozygous model, heterozygous model, recessive model, or allele model; of these three studies, one study19 reported ORs and 95% CIs in two different populations, which was treated as two distinct reports in the combined analysis.…”

Section: Resultsmentioning

confidence: 99%

Genetic association between the cyclin-dependent kinase inhibitor gene p27/Kip1 polymorphism (rs34330) and cancer susceptibility: a meta-analysis

Cheng

Wang²,

et al. 2017

Sci Rep

View full text Add to dashboard Cite

The p27 rs34330 (-79C/T) polymorphism has been widely studied for human cancer susceptibility. The current findings, however, still remained controversial. Therefore, we performed the meta-analysis to provide a more accurate result. Eligible studies were identified from PubMed database up to June 2015. The association of p27 rs34330 polymorphism and cancer susceptibility was estimated with odds ratios and corresponding 95% confidence intervals. The meta-analysis was performed with Stata 12. A total of ten studies with 11,214 cases and more than 8,776 controls were included in the meta-analysis (including breast, lung, thyroid, endometrial, and hepatocellular cancer). In pooled analysis, p27 gene rs34330 polymorphism significantly increased the cancer susceptibility. Subgroup analysis indicated that the elevated risk was observed under all the genetic models for Asians and under three genetic models for Caucasians. Results of sensitivity analysis were similar to the overall results. The results suggested that the p27 rs34330 polymorphism increased the cancer susceptibility, especially in Asians. Further well-designed and large sample size studies are warranted to verify the conclusion.

show abstract

“…p21 and p27 are famous cyclin-dependent kinase inhibitors. They are members in the Cip/Kip family, which can arrest the cell cycle and serve as tumor suppressors (21). Cell cycle arrest contributes to proliferation inhibition and apoptosis induction.…”

Section: Discussionmentioning

confidence: 99%

GSTM3 reverses the resistance of hepatoma cells to radiation by regulating the expression of cell cycle/apoptosis-related molecules

et al. 2014

View full text Add to dashboard Cite

Radiotherapy (RT) is a major modality of hepatoma treatment. However, liver tumors often acquire radioresistance, which contributes to RT failure. The exact mechanisms of the radioresistance in hepatoma cells are largely unknown. Glutathione S-transferase M3 (GSTM3) is a phase II transferase, however, recent studies have suggested that GSTM3 is a potential tumor suppressor. The purpose of the present study was to investigate the role of GSTM3 in reversing radioresistance, and to explore the molecular mechanism of this in the human radiation-resistant PRF/PLC/5R hepatocellular carcinoma (HCC) cell line. The radioresistant PLC/PRF/5R cells were used as cell model, and were derived from PLC/PRF/5 parental cells using fractionated irradiation. The radiosensitivity of the cells was tested by clonogenic assay and flow cytometry analyses. The expression of B-cell chronic lymphocytic leukemia/lymphoma 2 (Bcl-2), Bax, p21, p27 and p53 was analyzed by quantitative polymerase chain reaction and immunoblotting with or without radiation. The results showed that the expression levels of GSTM3 were significantly lower in the PLC/PRF/5R cells than in the PLC/PRF/5 parental cells. GSTM3 overexpression sensitized the PLC/PRF/5R cells to radiation mainly though induction of apoptosis. According to the evidence from Annexin-V/PI staining, it markedly increased the percentage of apoptotic PRF/PLC/5R cells. The clonogenic assay indicated that GSTM3 significantly decreased the RT survival fraction in PRF/PLC/5R cells. Furthermore, GSTM3 increased the expression of cell cycle- and apoptosis-related genes (Bcl-2, Bax, p21, p27 and p53) in PRF/PLC/5R cells with irradiation. These findings suggest that GSTM3 plays an pivotal role in reversing the radioresistance of HCC and may be a potential target for sensitizing HCC cells to RT. The underlying mechanisms may be linked to the cell cycle arrest and apoptosis facilitation.

show abstract

Genetic variants of p21 and p27 and hepatocellular cancer risk in a Chinese Han population: A case‐control study

Cited by 23 publications

References 52 publications

Polymorphisms of cell cycle control genes influence the development of sporadic medullary thyroid carcinoma

Polymorphisms of cell cycle control genes influence the development of sporadic medullary thyroid carcinoma

Genetic association between the cyclin-dependent kinase inhibitor gene p27/Kip1 polymorphism (rs34330) and cancer susceptibility: a meta-analysis

GSTM3 reverses the resistance of hepatoma cells to radiation by regulating the expression of cell cycle/apoptosis-related molecules

Contact Info

Product

Resources

About