Genetic variants of parvovirus B19 identified in the United Kingdom: Implications for diagnostic testing

Cohen, B. J.; Gandhi, Jayshree; Clewley, Jonathan P.

doi:10.1016/j.jcv.2006.01.011

Cited by 59 publications

(43 citation statements)

References 21 publications

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“…An MRCA occurring in the late 1950s is consistent with the epidemiology of genotype 1 and genotype 2. The large-scale surveillance of B19 virus genotypes in Europe showed that genotype 1 infections are almost invariably detected in contemporary samples, with extremely few if any genotype 2 (or 3) infections (6,7,21). This contrasts with the frequent detection of genotype 2 variants in tissue samples or persistently infected (immunosuppressed) individuals (16,24,27,30,36).…”

Section: Vol 82 2008 Rapid Evolution Of Parvovirus B19mentioning

confidence: 99%

“…Observations for maintained strong cytotoxic-T-cell reactivity in B19 virus-seropositive individuals (17) and low-level fluctuating viremia in 0.3 to 0.8% of healthy blood donors (20,35) do indeed imply various degrees of ongoing replication in vivo. It remains uncertain whether individuals with reactivated virus may be infectious; it is therefore possible that the infrequent cases of genotype 2 infections recorded in Europe (7,21,24) may have arisen by the transmission of reactivated virus from persistently infected individuals rather than by the continued low-level circulation of this genotype in the community. Understanding the nature of the persistence of B19 virus is therefore important for future investigations of its molecular epidemiology and evolution and of the factors underlying the large-scale population replacements that we have witnessed in the past few decades.…”

Section: Vol 82 2008 Rapid Evolution Of Parvovirus B19mentioning

confidence: 99%

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Rapid Sequence Change and Geographical Spread of Human Parvovirus B19: Comparison of B19 Virus Evolution in Acute and Persistent Infections

Norja

Eis‐Hübinger

Söderlund‐Venermo

et al. 2008

J Virol

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Parvovirus B19 is a common human pathogen maintained by horizontal transmission between acutely infected individuals. However, B19 virus can also be detected in tissues throughout the life of the host, although little is understood about the nature of such persistence. In the current study, we created large VP1/2 sequence data sets of plasma-and tissue (autopsy)-derived variants of B19 virus with known sample dates to compare the rates of sequence change in exogenous virus populations with those in persistently infected individuals. By using linear regression and likelihood-based methods (such as the BEAST program), we found that plasmaderived B19 virus showed a substitution rate of 4 ؋ 10 ؊4 and an unconstrained (synonymous)-substitution rate of 18 ؋ 10 ؊4 per site per year, several times higher than previously estimated and within the range of values for mammalian RNA viruses. The underlying high mutation frequency implied by these substitution rates may enable rapid adaptive changes that are more commonly ascribed to RNA virus populations. These revised estimates predict that the last common ancestor for currently circulating genotype 1 variants of B19 virus existed around 1956 to 1959, fitting well with previous analyses of the B19 virus "bioportfolio" that support a complete cessation of genotype 2 infections and their replacement by genotype 1 infections in the 1960s. In contrast, the evolution of B19 virus amplified from tissue samples was best modeled by using estimated dates of primary infection rather than sample dates, consistent with slow or absent sequence change during persistence. Determining what epidemiological or biological factors led to such a complete and geographically extensive population replacement over this short period is central to further understanding the nature of parvovirus evolution.

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Section: Vol 82 2008 Rapid Evolution Of Parvovirus B19mentioning

confidence: 99%

Section: Vol 82 2008 Rapid Evolution Of Parvovirus B19mentioning

confidence: 99%

Rapid Sequence Change and Geographical Spread of Human Parvovirus B19: Comparison of B19 Virus Evolution in Acute and Persistent Infections

Norja

Eis‐Hübinger

Söderlund‐Venermo

et al. 2008

J Virol

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“…Until recently, genotype 2 viraemic individuals were found relatively infrequently (Blümel et al, 2005;Cohen et al, 2006;Liefeldt et al, 2005;Nguyen et al, 1998Nguyen et al, , 1999Nguyen et al, , 2002Servant et al, 2002). Even in plasma factor concentrates, produced from thousands of blood donations, genotype 2 DNA was only detected in a very small percentage of individuals (Schneider et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

“…Genotype 3 virus was shown to be endemic in Ghana, West Africa (Candotti et al, 2004) and may be present in a certain region of Brazil (Sanabani et al, 2006). Outside these areas, only a few sporadic cases of viraemic infection have been reported in France (Nguyen et al, 1998(Nguyen et al, , 1999Servant et al, 2002) and one case was identified in the UK (Cohen et al, 2006). In an investigation performed in the USA, even the persisting genotype 3 DNA has only been found in a small percentage of tissue samples (Wong et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

Simultaneous persistence of multiple genome variants of human parvovirus B19

Schneider

Höne

Tolba

et al. 2008

Journal of General Virology

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The species human parvovirus B19 (B19V) can be divided into three genotypes. In this study, we addressed the question as to whether infection of an individual is restricted to one genotype. As viral DNA is detectable in tissue for long times after acute infection, we examined 87 liver specimens from adults for the presence of B19V DNA. Fifty-nine samples were found to be positive, 32 of them for genotype 1, 27 for genotype 2 and four for genotype 3. In four samples, DNA of two genotypes was detected; samples from three individuals were positive for genotypes 1 and 2 and a sample from one individual was positive for genotypes 1 and 3. Surprisingly, significant sequence heterogeneity was observed at approximately 1 % of the nucleotides of the genotype 1 genomes from individuals with double genotype 1 and 2 infection. Controls using different enzymes for genome amplification and dilutions of the template verified that nucleotide heterogeneity was due to the presence of three or more genome variants of genotype 1. In summary, the evidence shows that individuals can be infected with two different genotypes, and B19V DNA can persist as a population of different genomes. The results may have implications for the understanding of the antiviral immune response and the development of vaccines against B19V.

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“…5 Estas novas variantes foram encontradas na Europa Ocidental, EUA e Brasil, mas com menor prevalência que o genótipo 1. [5][6][7][8] Uma região epidêmica de genótipo 3 foi descrita em Ghana. 9,10 Figura 1.…”

Section: Introductionunclassified

Doenças hematológicas associadas ao eritrovírus

Garcia¹,

Pereira²,

Godoy³

et al. 2009

Rev. Bras. Hematol. Hemoter.

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O eritrovírus infecta células precursoras eritroides, determinando a interrupção temporária da eritropoese. Neste contexto, é importante o conhecimento das principais doenças hematológicas que podem estar associadas à presença do vírus, principalmente quando estão presentes em condições mórbidas, tais como nas anemias hemolíticas hereditárias. Este trabalho tem como objetivo relatar as principais doenças hematológicas que cursam com a infecção pelo eritrovírus B19. Rev. Bras. Hematol. Hemoter. 2009;31(4):285-290. Médica chefe do setor de Biologia Molecular da Fundação Pró-sangue, Hemocentro, São Paulo-SP. Palavras-chave: Eritrovírus; diagnóstico; doenças hematológicas. Revisão / Review REVISTA BRASILEIRA DE HEMATOLOGIA E H E M O T E R A P I A 1 Bióloga, aluna de pós-graduação do Laboratório de Imunopatologia da Faculdade de Medicina de São Paulo-SP. 2 Professora colaboradora da Disciplina e Serviço de Hematologia da Faculdade de Medicina da Universidade de São Paulo-SP. 3 Biomédica do Serviço de Hematologia do Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo-SP. 4 Biomédico. Aluno do curso de pós-doutorado da Faculdade de Medicina de São Paulo-SP. 5 Biomédico do Serviço de Hematologia do Hospital das Clínicas da Faculdade de Medicina da Universidade de São Faculdade de Medicina da Universidade de São Paulo (USP) -São Paulo-SP.Correspondência: Sheila de Oliveira Garcia Avenida Dr. Enéas de Carvalho Aguiar, bloco 4, 3085-22903085- E-mail: sheilagarcia.hp@gmail.com Doi:10.1590 Em 1984 foi relatado, na Inglaterra, o primeiro caso de infecção intrauterina por B19. Tratava-se de um caso de hidropisia fetal durante um surto epidêmico de eritema infeccioso. A partir deste relato, documentou-se a potencial toxicidade do eritrovírus B19 aos tecidos embrionários e fetais, agregando mais essa complicação ao grupo das doenças associadas a este agente. 2Desde então pode ser feita a associação do vírus com tropismo por células e tecidos, estes com alta taxa de multiplicação celular.Devido às características estruturais de seu DNA, o eritrovírus foi incluído na família Parvoviridae, subfamília Parvovirinae, gênero Erythrovirus pelo International Committee on Taxonomy of Viruses. 4Em 2002 foram descritos os genótipos 1 B19 clássico, genótipo 2, (protótipo K71 e cepa A6-similar) e genótipo 3 (protótipo V9). 5Estas novas variantes foram encontradas na Europa Ocidental, EUA e Brasil, mas com menor prevalência que o genótipo 1. [5][6][7][8] Uma região epidêmica de genótipo 3 foi descrita em Ghana. 9,10 286 Rev. Bras. Hematol. Hemoter. 2009;31(4):285-290 Garcia SO et al Classificação e estrutura viralO eritrovírus B19 é um vírus com cerca de 23nm, de estrutura icosaédrica (Figura 1), constituído por uma única fita de DNA não envelopado. 11A classificação na família Parvoviridae é feita por morfologia e função e o material genético constituído por uma fita simples de DNA. 12Devido à sua capacidade de infectar células de vertebrados e invertebrados, os Parvoviridae são divididos em Parvovirinae e Den...

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Genetic variants of parvovirus B19 identified in the United Kingdom: Implications for diagnostic testing

Cited by 59 publications

References 21 publications

Rapid Sequence Change and Geographical Spread of Human Parvovirus B19: Comparison of B19 Virus Evolution in Acute and Persistent Infections

Rapid Sequence Change and Geographical Spread of Human Parvovirus B19: Comparison of B19 Virus Evolution in Acute and Persistent Infections

Simultaneous persistence of multiple genome variants of human parvovirus B19

Doenças hematológicas associadas ao eritrovírus

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