Genetic variation among dengue 2 viruses of different geographic origin

Trent, Dennis W.; Grant, Joyce A.; Rosen, Léon; Monath, Thomas P.

doi:10.1016/0042-6822(83)90255-6

Cited by 93 publications

(69 citation statements)

References 23 publications

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“…There has been some discussion of the possible role of virus variation in determining the severity of dengue virus infection (Rosen, 1977) and it has been suggested that oligonucleotide fingerprinting may be a useful approach to understanding the biological variation associated with the disease (Trent et al, 1983). Although the number of viruses examined in this study was relatively small, there was no indication that isolates from patients with disease of similar severity were more closely related than other isolates.…”

Section: Discussioncontrasting

confidence: 40%

“…Trent et al (1983) reported that of five Burmese dengue-2 strains isolated in 1976, four represented a single topotype (Thailand-Burma) and one was closely related to Puerto Rico-South Pacific topotype viruses. The isolation of different topotypes of dengue-2 virus from Burkina Faso (formerly Upper Volta) has also been reported, although the isolations occurred at an interval of 2 years (Trent et al, 1983;Kerschner et al, 1986). Our study describes the extent of genetic variation within a topotype and indicates that different topotypes can circulate concurrently at a single geographic location in which dengue viruses are endemic.…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies of structural variation in dengue viruses have examined strains of diverse geographical and temporal origins and established the existence of clusters of genetic variants or topotypes within serotypes (Trent et al, 1983;Kerschner et al, 1986. Topotypes can be loosely defined as viruses that share about 80~ or more of large T 1 oligonucleotides and which are geographically or epidemiologicalty related.…”

Section: Discussionmentioning

confidence: 99%

“…The relationships between 12 geographically distinct dengue-type 1 isolates assessed by oligonucleotide mapping were such that isolates from the same geographical area clustered into groups which shared a high degree of oligonucleotide homology (Repik et al, 1983). Genetic variation in dengue-2 isolates was also examined by oligonucleotide fingerprinting and it was found that geographically separate and epidemiologically unrelated viruses were distinct (Trent et al, 1983). On the basis of these studies, genetic variants or topotypes of dengue-2 viruses were established representing strains which share 80~o or more of their large oligonucleotides.…”

Section: Introductionmentioning

confidence: 99%

“…On the basis of these studies, genetic variants or topotypes of dengue-2 viruses were established representing strains which share 80~o or more of their large oligonucleotides. Six topotypes have been reported to date representing strains from Puerto Rico-South Pacific, Thailand-Burma, the Seychelles, the Philippines, Jamaica-West Africa and Africa (Trent et al, 1983;Kerschner et al, 1986). Antigen signature analysis of these topotypes using a panel of monoclonal antibodies directed against viral structural proteins has indicated that viruses of different topotypes may have significant antigenic differences .…”

Section: Introductionmentioning

confidence: 99%

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Variation in Dengue Type 2 Viruses Isolated in Bangkok during 1980

Walker

Henchal

Blok

et al. 1988

Journal of General Virology

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SUMMARYDengue type-2 viruses isolated in metropolitan Bangkok during 1980 (Bangkok/80) were characterized by oligonucleotide fingerprinting, restriction enzyme (RE) mapping and antigenic analysis using monoclonal antibody probes. Of 10 isolates analysed by oligonucleotide fingerprinting, nine were very closely related, showing 72-5 ~o to 91.4 ~ oligonucleotide homology. One isolate (D80-141) produced a distinctly different fingerprint (55.7~ to 58"0~o homology) and was less related to other Bangkok/80 dengue-2 virus isolates than to a 1964 Bangkok isolate (16681). RE mapping conducted on complementary dsDNA prepared from three Bangkok/80 isolates, strain 16681 and the prototype New Guinea C strain confirmed that D80-141 was genetically distinct. On antigenic analysis, only one of 22 monoclonal antibody probes produced against representative 1980 Bangkok dengue-2 isolates, D80-100 and D80-141, was able to distinguish between these virus strains. Monoclonal antibody 47-10/10, prepared using D80-100 virus and directed at the NS1 non-structural glycoprotein, had a significantly lower (100-fold) solid phase radioimmune assay endpoint titre for D80-141 antigen than for D80-100 antigen. By the indirect immunofluorescence assay, 47-10/10 had lower antibody endpoint titres against D80-141, the NGC strain and 13 (12~o) of 110 Bangkok/80 isolates than to a control antibody preparation. These results suggest that strain D80-141 represents a second minor topotype of dengue-2 which was circulating concurrently with the major endemic topotype in Bangkok in early 1980.

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Section: Discussioncontrasting

confidence: 40%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Variation in Dengue Type 2 Viruses Isolated in Bangkok during 1980

Walker

Henchal

Blok

et al. 1988

Journal of General Virology

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Global Climate Change and Emerging Infectious Diseases

Patz¹

1996

JAMA

532

301

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Dengue haemorrhagic fever: Virus or host response?

Pang

1987

BioEssays

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The pathogenesis of dengue haemorrhagic fever has been the subject of intense research and considerable controversy. One hypothesisproposes that the immune response in a sensitized host is the primary mechanism. In contrast, others have suggested that the disease is caused by a more virulent, variant strain of dengue virus. Recent advances in molecular biology and hybridoma technology are providing valuable clues toward a solution and illustrating the fact that the course of a human viral disease is often a net result of unseparated interactions between host and virus.So why should the rest of the world be concerned at the plight of the American traveler? Local Donald Pevsners could raise similar hell to have the baggage weight restriction removed universally. It is also worth identifying the offending countries and their airlines as a warning to any traveler, regardless of nationality or destination, that when they check in for their flight they may expect to find strict adherence to the 44-pound weight limit and that the agent will not wink at, say 52 pounds and wave you on, especially in countries that are short of convertible currency. This is a means of gouging a not insignificant amount of extra income from the visitor.The standard excess baggage charge is 1 % of the first-class fare per kilogram. So the extra 20 pounds of baggage that you brought into Bucharest from New York would cost you around $200 to take out again.

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Genetic variation among dengue 2 viruses of different geographic origin

Cited by 93 publications

References 23 publications

Variation in Dengue Type 2 Viruses Isolated in Bangkok during 1980

Variation in Dengue Type 2 Viruses Isolated in Bangkok during 1980

Global Climate Change and Emerging Infectious Diseases

Dengue haemorrhagic fever: Virus or host response?

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