Genetic variation among methicillin-resistant Staphylococcus aureus isolates from cancer patients in Saudi Arabia

Ali, Alreshidi Mateq; Al‐Salamah, Ali A.; Hamat, Rukman Awang; Neela, Vasanthakumari; Alshrari, Ahmed Subeh; Atshan, Salman Sahab; AlAjlan, Hisham; Shamsudin, Mariana Nor

doi:10.1007/s10096-012-1801-9

Cited by 21 publications

(21 citation statements)

References 25 publications

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“…In the present study, 10 different spa types were identified, among which spa type t037 was the most prevalent, accounting for 23.3% of the isolates. This spa type was reported in previous studies conducted in Iran (10), China (29), and Saudi Arabia (30). It is noteworthy to mention that the majority of isolates (81%) with spa type t037 were MRSA.…”

Section: Discussionsupporting

confidence: 81%

SCCmec and spa Typing of Staphylococcus aureus Strains Isolated from Patients with Urinary Tract Infection: Emergence of spa Types t426 and t021 in Iran

Goudarzi

Abiri

Nasirian

et al. 2018

Jundishapur J Microbiol

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Background: Urinary tract infection (UTI) is one of the most frequent types of infections in community and hospital settings. Objectives: The aim of the present study was to investigate antimicrobial susceptibility patterns and also to determine spa and SCCmec types of Staphylococcus aureus strains isolated from patients with UTI. Methods: During a 12-month period, 863 urine samples were investigated. In vitro susceptibility of isolates was performed using the Kirby-Bauer disk diffusion method. Moreover, conventional PCR was performed to detect mecA, nucA, spa, and toxin (pvl, tst) genes as well as Multiplex PCR to determine SCCmec types. Results: A total of 90 isolates (10.4%) were analyzed in the present survey. Methicillin resistance was observed in 61.1% of the isolates. In total, 25 isolates (27.8%) were positive for the pvl-encoding gene and 40 (44.4%) for tst-1 encoding gene. Based on a multiplex PCR assay, the 4 different SCCmec types were detected as type III (38.9%), followed by type II (31.1%), type IV (28.9%), and type I (1.1%). PantonValentine Leukocidin (PVL) positive isolates belonged to SCCmec type IV (14.4%) and II (13.3%). The 90 S. aureus isolates were classified to 10 spa types t037 (23.3%), t924 (15.6%), t383 (15.6%), t426 (12.2%), t044 (12.2%), t790 (6.7%), t084 (4.4%), t021 (4.4%), t7580 (4.4%), and t064 (1.2%). The spa types t044, t790, and t084 were obtained from methicillin-resistant S. aureus (MRSA) strains and spa types t924 and t7580 from methicillin-sensitive S. aureus strains. Conclusions: To the best of the author's knowledge, this is the first study on spa types t426 and t021 in Iran. Since the majority of strains isolated from patients with UTI were MRSA, detection of MRSA clones and their characteristics is necessary.

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Section: Discussionsupporting

confidence: 81%

SCCmec and spa Typing of Staphylococcus aureus Strains Isolated from Patients with Urinary Tract Infection: Emergence of spa Types t426 and t021 in Iran

Goudarzi

Abiri

Nasirian

et al. 2018

Jundishapur J Microbiol

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“…Spa type t631 was the second frequent type in this study and also reported for the first time in Iran. In other studies, this spa type was reported with low frequency (45,49). It is interesting to note that all the t631 spa types in the present study were MRSA.…”

Section: Discussionsupporting

confidence: 67%

High frequency of multidrug‐resistant Staphylococcus aureus with SCCmec type III and Spa types t037 and t631 isolated from burn patients in southwest of Iran

Parhizgari

Khoramrooz

Hosseini

et al. 2015

APMIS

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Methicilin resistance Staphylococcus aureus (MRSA) infections are the major challenges in hospitals, especially in the burn units. The use of molecular typing methods is essential for tracking the spread of S. aureus infection and epidemiological investigations. The aim of this study was to find the profile of the spa types and also the prevalence of each SCCmec type of S. aureus strains in a central burn hospital in southwest of Iran. A total of 81 non-duplicate S. aureus were isolated from burn patients between April 2011 and February 2012. The susceptibility of the isolates against 13 different antibiotics was tested by disk agar diffusion (DAD) method. MRSA strains were identified by amplification of mecA gene. Multiplex-polymerase chain reaction (PCR) technique was used to determine the SCCmec types of MRSA strains and all the S. aureus isolates were typed by spa typing method. Detection of mecA gene showed that 70 (86.4%) of the isolates were MRSA. The highest rate of resistance was observed for penicillin (97.5%) and erythromycin (77.8%). None of the isolates were resistant to vancomycin. Sixty-seven of the 70 MRSA isolates harbored only SCCmec type III and three untypeable isolates. Five different spa types were detected. The most common spa types were t037 (42.5%) and t631 (34.5%) and were only found in MRSA isolates. Only SCCmec type III was found in burn patients which emphasizes the HA-MRSA origin of these strains. Only five different spa types identified in this study are in accordance with one SCCmec type which indicates that a limited number of bacterial colons are circulated in the burn unit in this hospital.

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“…The ability of S. aureus to cause a spectrum of diseases both in humans and animals is due to a combination of bacterial virulence factors [6]. There are several virulence factors secreted by S. aureus including Panton-Valentine Leukocidin (PVL), protein A, toxic shock syndrome toxin 1 (TSST-1), and exfoliative toxins and several other toxins [3].…”

Section: Introductionmentioning

confidence: 99%

“…They may differ in the arrangement of chromosomal alleles, or the content of variable accessory genetic elements that confer certain resistance traits on the pathogen. Therefore, it is of prime importance to understand the genetic characteristics of these MRSA isolates which could be used to control these pathogens [6].…”

Section: Introductionmentioning

confidence: 99%

Antibiograms, toxin profiling and molecular typing of Staphylococcus aureus isolates from two tertiary hospitals in Jordan

Jaradat

Hamdan

Hayajneh

et al. 2017

J Infect Dev Ctries

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Introduction: Methicillin-resistant Staphylococcus aureus (MRSA) is a major cause of hospital-associated infections. This study was undertaken to investigate toxin profiles as well as antibiotic resistance patterns of S. aureus isolates form two tertiary hospitals in Jordan. Methodology: A total of 250 S. aureus isolates from clinical samples of two tertiary hospitals were analyzed for the presence of the mecA, vanA, vanB, and 16 Staphylococcus toxin encoding genes using PCR. The isolates were further tested for antimicrobial sensitivities using the disc diffusion method. DNA from all the isolates were fingerprinted by coa gene Restriction Fragment Length Polymorphism (RFLP) to study relationships between isolates from the two hospitals. Results: 73.2% of the isolates contained the mecA gene and thus were designated MRSA. All MRSA isolates showed high levels of resistance to many of the antibiotics compared to those of MSSA. All MRSA isolates were susceptible to vancomycin and teicoplanin while all MSSA isolates were susceptible to nitrofurantoin, teicoplanin, vancomycin, cefoxitin, clindamycin, erythromycin and gentamycin. The isolates exhibited high prevalence of the toxin genes and none of the isolates contained less than 4 genes with one isolate contained 14 genes with no apparent differences in gene profiles among MRSA and MSSA. About 60% of the isolates contained 12 to 13 toxin genes and were isolated either from pus or blood. Conclusion: Antibiograms of the MRSA isolates were significantly different from MSSA antibiograms while there were no apparent differences in the toxin genes profiles. Further, coagulase gene RFLP of the isolates showed that the isolates are very heterogenic.

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Genetic variation among methicillin-resistant Staphylococcus aureus isolates from cancer patients in Saudi Arabia

Cited by 21 publications

References 25 publications

SCCmec and spa Typing of Staphylococcus aureus Strains Isolated from Patients with Urinary Tract Infection: Emergence of spa Types t426 and t021 in Iran

SCCmec and spa Typing of Staphylococcus aureus Strains Isolated from Patients with Urinary Tract Infection: Emergence of spa Types t426 and t021 in Iran

High frequency of multidrug‐resistant Staphylococcus aureus with SCCmec type III and Spa types t037 and t631 isolated from burn patients in southwest of Iran

Antibiograms, toxin profiling and molecular typing of Staphylococcus aureus isolates from two tertiary hospitals in Jordan

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