Genetic variation and microevolution of dengue 2 virus in Southeast Asia

Trent, Dennis W.; Grant, Joyce A.; Monath, Thomas P.; Manske, Charles L.; Corina, Marc; Fox, George E.

doi:10.1016/0042-6822(89)90195-5

Cited by 43 publications

(31 citation statements)

References 36 publications

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“…Molecular studies have demonstrated that dengue viruses vary genetically in nature; unfortunately, phenotypic changes that have been observed in the field have not yet been associated with genetic changes in the virus (26,99,100,116,117,143). Collectively, however, the data suggest that viral factors play a significant role in the pathogenesis of severe dengue disease.…”

Section: Virologic Factorsmentioning

confidence: 81%

Dengue and Dengue Hemorrhagic Fever

Gubler¹

1997

Tropical Infectious Diseases

633

756

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Section: Virologic Factorsmentioning

confidence: 81%

Dengue and Dengue Hemorrhagic Fever

Gubler¹

1997

Tropical Infectious Diseases

633

756

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“…Dengue-2 virus has received the most attention because of its frequent association with severe dengue symptoms. Two exhaustive analyses of Thai dengue-2 viruses demonstrated antigenic and genetic variation within a defined geographic area but no virulence-related marker was identified [35,36]. Comparisons of structural genes of dengue-2 viruses isolated from patients with different levels of disease severity demonstrated a high degree of heterogeneity in the nucleotide and amino acid sequences but no correlation with the degree of virulence could be established [3,[30][31][32].…”

Section: Introductionmentioning

confidence: 94%

Direct sequencing of genomic cDNA fragments amplified by the polymerase chain reaction for molecular epidemiology of dengue-2 viruses

Denis

Nogueira

Drouet

et al. 1993

Archives of Virology

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A nucleotide fragment encoding amino acids 29 to 94 in the E-protein of 28 dengue-2 isolates of diverse geographic and host origins was examined by direct sequencing of a polymerase chain reaction (PCR)-amplified product, and compared to six previously published sequences. Nucleotide divergence ranged from 0 to 19.8% corresponding to a maximum of 9% divergence in the amino acid sequence. Taking a divergence of 6% between the nucleotide sequence as a cut off for genotype classification, six groups have been established. Southeast Asian and the Jamaican 1983 genotypes show a high rate of similarity (> 95.2%). Our results suggest that virus of this group is now circulating as the dominant topotype in Brazil (1990) and in French Guyana (1986-1991). African strains fall into two groups, one endemic group (1970-1990) and one epidemic group (1986-1987). The three other groups correspond to viruses from Sri Lanka (1982) and the Seychelles (1977), from Puerto Rico (1973) and from Tahiti (1975). Our approach appears to be valuable characterizing dengue isolates, easily and rapidly.

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“…Rapid evolution of R N A viruses makes it possible, by comparing genomic sequences, to detect epidemiological links between strains even if they have been isolated in remote geographical regions. Using oligonucleotide fingerprinting it is possible to survey the entire genome and this method has been widely used in epidemiological studies (Kew et al, 1984;Takeda et al, 1984;Trent et al, 1989). However, it loses its power relatively rapidly as strains diversify.…”

Section: Discussionmentioning

confidence: 99%