Genetic Variation in Morphology, Seed Quality and Self-(in)Compatibility among the Inbred Lines Developed from a Population Variety in Outcrossing Yellow Mustard (Sinapis alba)

Abstract

Yellow mustard (Sinapis alba L.) has been grown as an important source of condiment for the spice trade in the world. It is an obligate outcrossing species due to its sporophytic self-incompatibility (SI). To utilize heterosis for yield potential, we have attempted to develop elite component inbred lines for producing high-yielding synthetic varieties for this crop. The open-pollinated variety Andante was used as the initial population. To circumvent the SI barrier, bud-pollination for selfing was performed on the selected initial (S0) plants. Various types of inbreeding depression were observed in the S1 generation. Elite inbred lines tolerant to inbreeding were produced by purging the deleterious alleles in each inbred generation. Self-compatible (SC) lines were developed for the first time in this species. There were three types of erucic variants (high: 49.9%, median: 23.9% and low: 1.4%), three types of linolenic variants (high: 18.5%, median: 13.8% and low: 3.8%) and two types of mucliage variants (high: 164.0 cS*mL/g and low: 12.0 cS*mL/g) among the developed inbred lines. These variants are being used to investigate the genetic and molecular mechanism underpinning the phenotypic variation of the seed oil profile and SI/SC traits in yellow mustard.

Genetic diversity analysis of yellow mustard (Sinapis alba L.) germplasm based on genotyping by sequencing

“…Canadian breeding has been focusing on seed-yield increase since the 1950s (Downey and Rakow 1995;Katepa-Mupondwa et al 2005). As yellow mustard is an obligate outcrossing species (Olsson 1960), recurrent selection has been a widely used breeding method (Cheng et al 2012), but it is not always effective for seed yield due to low heritability. To enhance breeding efforts, diverse accessions of yellow mustard germplasm have been collected from different parts of the world, and 132 yellow mustard accessions are now maintained at Plant Gene Resources of Canada (PGRC) at Saskatoon.…”

Phenomenon of Inbreeding Depression on Maize in Perspective of The Quran

“…The origin plant is so-called S 0 , and the progeny from self-pollination of the plant is so-called S 1 (progeny from selfpollinationof the first generation). Progeny from self-pollination of the second generation is socalled S 2 , and so on (Cheng, Williams, & Zhang, 2012;Jaradat & Goldstein, 2018;Kustanto, Basuki, Sugiharto, & Kasno, 2012;Poehlman, 1979). The lost vigor during self-pollination period was regained on progeny F 1 when the inbred line was crossbred with other inbred lines, which had no correlation (Pekkala, Knott, Kotiaho, Nissinen, & Puurtinen, 2014;Poehlman, 1979).…”

Transposable Element Insertion and Epigenetic Modification Cause the Multiallelic Variation in the Expression of FAE1 in Sinapis alba

“…Erucic acid variants have been identified by inbreeding of open-pollinated plants in yellow mustard, which is an obligate outcrossing crop due to its self-incompatible reproductive system (Raney et al, 1999;Cheng et al, 2012). The high (52.9%), medium (24.4%), low (1.4%), and zero (0.1%) erucic contents of the yellow mustard lines Y517, Y496, Y1130, and Y514 were conditioned by the four FAE1 alleles E 1 , E 2 , E 3 , and e, respectively (Javidfar and Cheng, 2013).…”