Genetic variation in New Zealand populations of the plant pathogenSclerotinia sclerotiorum

Abstract

Genetic variation of the fungal plant pathogen Sclerotinia sclerotiorum (Lib.) de Bary was examined using DNA fingerprinting. Seventyfive isolates of S. sclerotiorum were collected from four populations in the South Island of New Zealand. DNA fingerprints were generated for each isolate by Southern blotting using a cloned repetitive sequence, pLK44.20, as a probe. The 47 different fingerprints produced revealed a high level of variation both within and between populations. Comparison of fingerprint similarities indicated substantial local movement of isolates but gave little evidence for long-range dispersal. A subset of the isolates was tested for mycelial compatibility. Pairs with identical or highly similar DNA fingerprints gave compatible reactions, whereas isolates whose fingerprints were dissimilar were incompatible. The high level of variation observed in New Zealand populations of S. sclerotiorum has relevance for control of diseases caused by this pathogen, as any method of † Author to whom correspondence is to be addressed. H98027Received 12 June 1998; accepted 3 November 1998 disease control must be effective across the range of pathogen variation.

Genetic diversity and morphological variability of Sclerotinia sclerotiorum isolates of oilseed Brassica in India

“…Variability among the isolates has already been reported by Carpenter et al (1999). All isolates were darkly pigmented.…”

Case Study: Sclerotinia sclerotiorum: Genetic Diversity and Disease Control

“…Prior researches on S. sclerotiorum population structure, in areas where canola, sunflower, cabbage, and other crops are grown, have shown that this pathogen populations are mainly clonal (Kohli et al, 1992(Kohli et al, , 1995Anderson and Kohn, 1995;Cubeta et al, 1997;Kohli and Kohn, 1998;Carpenter et al, 1999;Hambleton et al, 2002), even though some recombination has been detected (Cubeta et al, 1997;Carbone and Kohn, 2001a), being more evident in subtropical populations than in temperate ones (Carbone et al 1999;Carbone and Kohn, 2001b;Atallah et al, 2004;Sexton and Howlett, 2004;Sexton et al, 2006;Mert-Türk et al, 2007;Gomes et al, 2011).…”

“…Several works have deployed MCG typing in population studies of both S. sclerotiorum and Sclerotinia minor (Kohli et al, 1992(Kohli et al, , 1995Cubeta et al, 1997;Carpenter et al, 1999;Carbone and Kohn, 2001b;Hambleton et al, 2002;Phillips et al, 2002;Durman et al, 2003;Hollowell et al, 2003;Kull et al, 2004;Atallah et al, 2004;Sexton and Howlett, 2004). Individual isolates are classified into clonal lineages by the use of two or more independent markers such as MCGs, DNA fingerprinting, or microsatellite markers (Kohn et al, 1991;Carbone et al, 1999;Sirjusingh and Khon, 2001;Hambleton et al, 2002;Auclair et al, 2004).…”

Genetic Diversity among Different Isolates of Sclerotinia sclerotiorum in North of Iran

“…Oilseed rape is a new crop to this area and the high level of polymorphism may reflect the movement of S. sclerotiorum ascospores onto rapeseed crop from several wild host plants. 28 …”