Genetic variation in susceptibility to pine wilt disease of maritime pine (Pinus pinaster Aiton) half-sib families

Carrasquinho, Isabel; Lisboa, Ana; Inácio, Maria L.; Gonçalves, Elsa

doi:10.1007/s13595-018-0759-x

Cited by 29 publications

(53 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Pinus pinaster seeds were obtained from a top genetically ranked plus tree for PWD resistance (family 152) belonging to a breeding population, and ranked on the 1st EBLUP position out of 96. The mass selection program was performed at “Herdade da Comporta” (38°21′28.52″N; 8°45′49.89″W) in southern Portugal [ 9 , 62 ]. For cold-wet stratification, seeds were placed on a double layer of filter paper soaked in sterile distilled water, in Petri dishes sealed with Parafilm ® , and kept in the dark for 3 weeks at 4 °C.…”

Section: Methodsmentioning

confidence: 99%

“…In this study, resistance to PWN infection was considered when inoculated plants showed no PWD external symptoms (0%), whereas the symptomatic plants were considered susceptible to PWN infection. As the definition of resistant and tolerant plants cannot be differentiated based solely on external symptoms, plants considered as resistant in this study include their ability to defend or withstand the pathogenic attack, as in Carrasquinho et al [ 9 ].…”

Section: Methodsmentioning

confidence: 99%

“…Although variation in resistance to PWN infection between and within European Pinus spp. has been documented [ 6 , 7 , 8 , 9 , 10 ], little is known about the biochemical mechanisms underlying differential tree susceptibility.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Pinus pinaster Early Hormonal Defence Responses to Pinewood Nematode (Bursaphelenchus xylophilus) Infection

et al. 2021

Self Cite

View full text Add to dashboard Cite

The pinewood nematode (PWN) is the causal agent of pine wilt disease, a pathology that affects conifer forests, mainly Pinus spp. PWN infection can induce the expression of phytohormone-related genes; however, changes at the early phytohormone level have not yet been explored. Phytohormones are low-abundance metabolites, and thus, difficult to quantify. Moreover, most methodologies focus mainly on Arabidopsis or crop species. This work aimed to validate a fast (run time 6.6 min) liquid chromatography-triple quadrupole tandem mass spectrometry (LC-QqQ-MS/MS) analytical method to quantify 14 phytohormones in Pinus pinaster stem tissues. This method was further applied to evaluate, for the first time, early phytohormone changes in susceptible and resistant phenotypes of P. pinaster 24, 48 and 72 h after inoculation (HAI) with PWN. A significant increase in salicylic acid (SA, 48 and 72 HAI) and jasmonic acid methyl ester (JA-ME, 72 HAI) was observed in susceptible phenotypes. Results indicate that the higher susceptibility of P. pinaster to PWN infection might result from an inefficient trigger of hypersensitive responses, with the involvement of JA and SA pathways. This work provides an important update in forest research, and adds to the current knowledge of Pinus spp. defence responses to PWN infection.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Pinus pinaster Early Hormonal Defence Responses to Pinewood Nematode (Bursaphelenchus xylophilus) Infection

et al. 2021

Self Cite

View full text Add to dashboard Cite

show abstract

“…Remarkably, varieties with high resistance levels have been described in susceptible pine species (Toda and Kurinobu, 2002;Xu et al, 2012). In P. pinaster, different levels of resistance were also observed in plants after artificially inoculated with PWN (Menéndez-Gutiérrez et al, 2017a,b;Carrasquinho et al, 2018). Since control measures implemented so far have failed in stopping PWD spreading, breeding resistant varieties may be a highly effective control strategy.…”

Section: Introductionmentioning

confidence: 99%

“…Breeding programs have been successfully implemented for Pinus thunbergii, Pinus densiflora, and Pinus massoniana (Toda and Kurinobu, 2002;Xu et al, 2012). For P. pinaster, genetic variation in susceptibility to PWN inoculation was observed in two independent studies (Menéndez-Gutiérrez et al, 2017a;Carrasquinho et al, 2018), in which a moderate family heritability for survival (0.37; Carrasquinho et al, 2018) and mortality (0.59; Menéndez-Gutiérrez et al, 2017a) after inoculation was detected, suggesting that implementation of breeding programs can be valuable. Furthermore, plants without symptoms had very few PWNs when compared to symptomatic plants (Menéndez-Gutiérrez et al, 2017a), suggesting asymptomatic plants were able to control the multiplication of PWN, showing, therefore, true resistance to the parasite (Trudgill, 1991;Woodcock et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Insights Into the Mechanisms Implicated in Pinus pinaster Resistance to Pinewood Nematode

et al. 2021

Self Cite

View full text Add to dashboard Cite

Pine wilt disease (PWD), caused by the plant–parasitic nematode Bursaphelenchus xylophilus, has become a severe environmental problem in the Iberian Peninsula with devastating effects in Pinus pinaster forests. Despite the high levels of this species' susceptibility, previous studies reported heritable resistance in P. pinaster trees. Understanding the basis of this resistance can be of extreme relevance for future programs aiming at reducing the disease impact on P. pinaster forests. In this study, we highlighted the mechanisms possibly involved in P. pinaster resistance to PWD, by comparing the transcriptional changes between resistant and susceptible plants after infection. Our analysis revealed a higher number of differentially expressed genes (DEGs) in resistant plants (1,916) when compared with susceptible plants (1,226). Resistance to PWN is mediated by the induction of the jasmonic acid (JA) defense pathway, secondary metabolism pathways, lignin synthesis, oxidative stress response genes, and resistance genes. Quantification of the acetyl bromide-soluble lignin confirmed a significant increase of cell wall lignification of stem tissues around the inoculation zone in resistant plants. In addition to less lignified cell walls, susceptibility to the pine wood nematode seems associated with the activation of the salicylic acid (SA) defense pathway at 72 hpi, as revealed by the higher SA levels in the tissues of susceptible plants. Cell wall reinforcement and hormone signaling mechanisms seem therefore essential for a resistance response.

show abstract

Metabolomics ofPinusspp. in Response to Pinewood Nematode Infection

Rodrigues,

da Silva,

Vasconcelos

et al. 2023

Monitoring Forest Damage With Metabolomics Methods

View full text Add to dashboard Cite

Genetic variation in susceptibility to pine wilt disease of maritime pine (Pinus pinaster Aiton) half-sib families

Cited by 29 publications

References 29 publications

Pinus pinaster Early Hormonal Defence Responses to Pinewood Nematode (Bursaphelenchus xylophilus) Infection

Pinus pinaster Early Hormonal Defence Responses to Pinewood Nematode (Bursaphelenchus xylophilus) Infection

Insights Into the Mechanisms Implicated in Pinus pinaster Resistance to Pinewood Nematode

Metabolomics ofPinusspp. in Response to Pinewood Nematode Infection

Contact Info

Product

Resources

About