Energy content is an important criterion for evaluation in lucerne breeding.
The in sacco method was used to investigate the
phenotypic variation in the kinetics of degradation of dry matter and fibre
for 15 lucerne cultivars grown in one location, and to compare these kinetic
parameters with laboratory analyses of dry matter solubility, fibre content
(NDF, neutral detergent fibre), and fibre solubility. Bags containing forage
samples were incubated for 2, 4, 8, 12, 24, 48, and 72 h in 3 fistulated
Holstein cows. Dry matter and NDF degradation curves were modelled with a
sigmoid curve with 3 parameters. Phenotypic variations were observed for dry
matter and NDF disappearance at each time interval, and for the parameters of
the degradation kinetics. For both dry matter and NDF, the totally degraded
fraction was correlated with the solubility measured by wet chemistry, but the
rate of degradation of the slowly degraded fraction was not correlated with
chemical analyses. The effect of the variations in the parameters of dry
matter and fibre degradation on ruminant performance needs to be studied
further.