Genetic Variation of Asian Tapir

Abstract

2 A 24 HSRVII) ?V-A"90iz'ftsI# Ere)iCHR i), ne)ZIHSs!,! Z), flxlilws-3) 1) matLF'i,Mvasuiz)f-9-i24].0804 pmAJI[veuaDltiJg[IIJ[[#tla'MI 155-1 z) waata/i"A l･ISSigittzmu i 241-oool t4if,EMvaIfi"trlrfimaX.t.FltNUy ll7s-1 3) .gpafu#tuUpa i 191-O042 HIJJ;'glSH!irftUe,t71 7-1-1 ABSTRACT. For the geneLic managemeni or capt.ive Asian tapir, geneLic variation of captive indjviduals was investigated using mitochondrial cytochrorno b scquencing. We t'ound two genetic lines/ the Indonesian captiviLy and Malay Peninsu[a captivity lines, This finding indicates that the Asian tapir rnight have difi/erentiated into two geogi'aphic groups, name!y, the Sumatra Island and Malay Peninsula groups, This biogeograpbic diff{/rent.iation is also observed in other maminals iri this area, Key wordsi Asian Lapir, capLive conservation, mitochoridrial cytochrome b

Use of mitochondrial cytochrome b sequences to determine the origin of captive Asian tapirs Tapirus indicus: implications for conservation

“…The majority of the captive individuals (48.4%) were haplotype Ti-1, which was a new haplotype found only in Thailand. Ti-2 and Ti-3 were similar to those described by Ogata et al (2009) This information enabled us to distinguish haplotypes (blue circles) according to geographic region: 1 haplotype was found on the island of Sumatra, Indonesia and 2 haplotypes were found only on the Malay peninsula (Malaysia). One grouping of a large population (blue rectangle) based on longer mitochondrial cytochrome b gene sequences (975 bp) with 12 sites was grouped into Ti-1 and Ti-2 (Thailand), Ti-3 (Thailand/ Malaysia), and Ti-4 (unknown origin).…”

“…Ti-5 and Ti-6 were found on the Malay Peninsula (Rovie-Ryan et al 2008). Ti-7 was located only on the island of Sumatra in Indonesia (Ogata et al 2009). Ti-4 indicated conserved and variable sites, which were quite similar to tapirs originating from mainland Indochina.…”

“…Moreover, the mitochondrial cytochrome b gene has been reliably used for assessing genetic variation in mammals due to its moderate evolutionary rate. Previous studies of Asian tapirs have shown 6 haplotypes with 17 variable sites (n = 29) using available cytochrome b sequences compiled from GenBank (Pitra & Veits 2000, Rovie-Ryan et al 2008, Ogata et al 2009, Steiner & Ryder 2011. The distribution of each haplotype shows that the intra-species relationship is largely influenced by the Isthmus of Kra and the Malacca Strait.…”

“…Previous genetic studies of the family Tapiridae have examined mitochondrial genes such as cytochrome c oxidase subunit I, cytochrome c oxidase subunit II (Ashley et al 1996), 12S rRNA (Springer 1997, Norman & Ashley 2000, Murphy et al 2001), 16S rRNA (Murphy et al 2001, O'Leary & Gatesy 2008, and cytochrome b (Pitra & Veits 2000, Arnason et al 2008, Rovie-Ryan et al 2008, Ogata et al 2009, De Thoisy et al 2010, Sanches et al 2011, Steiner & Ryder 2011. Each coding gene has provided a different degree of phylogenetic resolution at the taxonomic level.…”

Genetic diversity of the captive Asian tapir population in Thailand, based on mitochondrial control region sequence data and the comparison of its nucleotide structure with Brazilian tapir

“…Little genetic information is known about the wild and captive Asian tapir populations (Rovie-Ryan et al 2008;Ogata et al 2009;Muangkram et al 2013b). Recently, the full length of complete mitochondrial genome is elucidated by our previous study (Muangkram et al 2016).…”