Genetic variation of carotenoids, vitamin E and phenolic compounds in Provitamin A biofortified maize

Muzhingi, Tawanda; Palacios-Rojas, Natalia; Miranda, A.; Cabrera, María Luisa; Yeum, Kyung‐Jin; Tang, Guangwen

doi:10.1002/jsfa.7798

Cited by 62 publications

(55 citation statements)

References 35 publications

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“…However, none of these approaches had been viable due to ineffective distribution system, poor infrastructure and/or non‐affordability by the poor people (Tanumihardjo, Palacios‐Rojas, & Pixley, ). Biofortification, the process of breeding micronutrient dense staple crops, is cost‐effective, sustainable and micronutrients can be delivered among the target group in its natural form (Bouis & Welch, ; Gupta et al., ; Muzhingi et al., ).…”

Section: Introductionmentioning

confidence: 99%

Influence of rare alleles of β‐carotene hydroxylase and lycopene epsilon cyclase genes on accumulation of provitamin A carotenoids in maize kernels

et al. 2017

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Vitamin A deficiency is one of the major health problems worldwide. Traditional yellow maize possesses very low provitamin A (proA) concentration in endosperm. The influence of rare alleles of β‐carotene hydroxylase (crtRB1) and lycopene epsilon cyclase (lcyE) genes capable of enhancing proA concentration was studied in four BC2F2 populations generated using subtropical inbreds and CIMMYT‐HarvestPlus lines. The occurrence of severe segregation distortion for crtRB1 gene was observed, while lcyE gene was segregated as per Mendelian ratio. Genotype with favourable allele of crtRB1 (CC) had a significant effect on β‐carotene (BC) (7.9‐fold), β‐cryptoxanthin (BCX) (twofold) and proA (5.5‐fold) accumulation, compared to unfavourable genotype (C+C+). Genotype with favourable allele of lcyE (LL) showed 2.1‐fold, 1.6‐fold and twofold significant enhancement in BC, BCX and proA, respectively, over unfavourable genotype (L+L+) in pooled analysis. Of the nine genotypes, double homozygote (CC/LL) had the highest mean BC (12.60 μg/g), BCX (4.44 μg/g) and proA (14.82 μg/g), and combined effect was significantly better than individual gene effects or any other combinations. The information generated here would be useful in designing strategy for proA enrichment in subtropical maize.

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Section: Introductionmentioning

confidence: 99%

Influence of rare alleles of β‐carotene hydroxylase and lycopene epsilon cyclase genes on accumulation of provitamin A carotenoids in maize kernels

et al. 2017

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“…Another research comprehends 87 inbred lines from the main heterotic groups in China and revealed βcarotene content in range from 0.016-1.726 µg/g DW (Chander et al, 2008), much lower than obtained in inbreds in our study. In maize hybrids biofortified for vitamin A (Kurilich and Juvik, 1999) β-carotene content was 0.55 µg/g DW, and in new 36 provitamin A biofortified hybrids (Muzhingi et al, 2017) β-carotene was within range from 1.3-8.00 µg/g DW. Provitamin A (α-carotene, β-carotene, βcryptoxanthin) and non-provitamin A carotenoids (lutein and zeaxanthin) in maize are primarly located in the endosperm, but tocopherols (α-tocopherol, γ-tocopherol and δ-tocopherol) are located in the embryo.…”

Section: Resultsmentioning

confidence: 94%

“…Higher carotenoid content could be estimated based on grain color, but not determineted, because of possible accumulation in different parts of maize kernel (seed coat, endosperm or germ). However, maize is not only a good source for provitamin A carotenoids but also a source of vitamin E, tocochromanols and phenolic compounds, which have antioxidant properties (Muzhingi et al, 2017). Vitamin E is common name for eight naturally occurring compounds, lipidsoluble antioxidants with two distinct groups, tocopherols and tocotrienols (Della Penna, 2006).…”

Section: Introductionmentioning

confidence: 99%

Pigmented maize - a potential source of β-carotene and α-tocopherol

Violeta¹,

Mesarović²,

Srebrić³

et al. 2018

J. eng. process. manag.

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Among cereals, maize has the highest content of bioavailable micronutrients in grain, particularly β-carotene and α-tocopherol, which makes this crop the most appropriate for biofortification. Great genetic variability is a valuable source of micronutrients, and genotypes with enhanced grain content could be used for improvement of commercial hybrids or synthetic populations creation. Three populations with dark orange, dark red and red grain, five elite lines, and their crosses were evaluated for β-carotene and α-tocopherol content. Based on obtained results, line (L5) could be further used in breeding for increased β-carotene content, and population with dark orange grain (P1) is recommended as a good source for multi-nutrient biofortification for both β-carotene and α-tocopherol. Three lines (L1, L2 and L5) had significantly higher value of α-tocopherol in crosses with dark red population (P2), compared to lines per se, and require several cycles of back-crossing for increase nutrient content.

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“…Wide genetic variation in tocopherol components has been observed in maize (Rocheford et al, 2002;Egesel et al, 2003;Li et al, 2012;Feng et al, 2015;Muzhingi et al, 2017;Das et al, 2019a). Estimation of tocopherol fractions (a-, b-, g-, and d-) is simple and fast (~15 min/sample) using HPLC, but it involves high cost (US$25-30 per sample).…”

Section: Molecular Breeding For Vitamin E Enrichmentmentioning

confidence: 99%

Molecular Breeding for Nutritionally Enriched Maize: Status and Prospects

et al. 2020

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Maize is a major source of food security and economic development in sub-Saharan Africa (SSA), Latin America, and the Caribbean, and is among the top three cereal crops in Asia. Yet, maize is deficient in certain essential amino acids, vitamins, and minerals. Biofortified maize cultivars enriched with essential minerals and vitamins could be particularly impactful in rural areas with limited access to diversified diet, dietary supplements, and fortified foods. Significant progress has been made in developing, testing, and deploying maize cultivars biofortified with quality protein maize (QPM), provitamin A, and kernel zinc. In this review, we outline the status and prospects of developing nutritionally enriched maize by successfully harnessing conventional and molecular marker-assisted breeding, highlighting the need for intensification of efforts to create greater impacts on malnutrition in maize-consuming populations, especially in the low-and middle-income countries. Molecular marker-assisted selection methods are particularly useful for improving nutritional traits since conventional breeding methods are relatively constrained by the cost and throughput of nutritional trait phenotyping.

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Genetic variation of carotenoids, vitamin E and phenolic compounds in Provitamin A biofortified maize

Abstract: Genotype was significant in determining the variation in β-cryptoxanthin, β-carotene, α-tocopherol and γ-tocopherol contents (P < 0.01). A genotype × environment interaction was observed for γ-tocopherol content (P < 0.01). © 2016 Society of Chemical Industry.

Cited by 62 publications

References 35 publications

Influence of rare alleles of β‐carotene hydroxylase and lycopene epsilon cyclase genes on accumulation of provitamin A carotenoids in maize kernels

Influence of rare alleles of β‐carotene hydroxylase and lycopene epsilon cyclase genes on accumulation of provitamin A carotenoids in maize kernels

Pigmented maize - a potential source of β-carotene and α-tocopherol

Molecular Breeding for Nutritionally Enriched Maize: Status and Prospects

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