Genetic Variation of Foxtail Millet [Setaria italica (L.) P. Beauv.] Among Accessions Collected From Korea Revealed by AFLP Markers

Abstract

AFLP markers were employed to detect genetic diversity and genetic relationship among 26 accessions of foxtail millet collected from Korea. Analysis of 26 accessions of foxtail millet with nine AFLP primer set combinations detected a total of 170 bands, of which 145 (85.3%) showed polymorphic at the species level. The phenotypic diversity (H S ) calculated for the nine primer combinations ranged from 1.84 to 6.8, with an average of 3.85. The average phenotypic diversity values were 3.39 and 2.99 for accessions collected from Gangwon province (Group 1), and accessions collected from the other areas including Gyeonggi province (Group 2), respectively. In the cluster analysis of 26 accessions, two major groups were recognized at 73% genetic similarity. Group I includes 13 accessions, which were collected in Gangwon province, and 1 accession, which was collected in Gyeonggi province, with genetic similarity of 76.8%. Group II includes two accessions, which were collected in Gangwon province, and 10 accessions, which were collected in the other areas with genetic similarity of 78.9%. The presenting data on the assessment of genetic diversity and genetic relationships among Korean accessions of foxtail millet will be helpful for efficient collection or conservation strategy of foxtail millet germplasm in Korea.

EST-SSR Based Genetic Diversity and Population Structure among Korean Landraces of Foxtail Millet (Setaria italica L.)

“…1). However, for the majority of the landraces, no clear corresponding relationship between clustering groups and their geographic locations was found, and most of them had tendency to be grouped together. The landraces from Gangwon-do spanned over three clusters showing higher diversity, which is similar to the results reported by Kim et al (2011) for the germplasm collections from same region. Additionally, the suitable climate and soil of this area have been useful for the farmers to grow and maintain foxtail millet landraces .…”

“…Many different types of markers such as isozyme (Jusuf and Pernes, 1985), restriction fragment length polymorphism (RFLP) , RAPD (Schontz and Reather, 1999), AFLP (Kim et al, 2011), and simple sequence repeat (SSR) markers (Jia et al, 2009) have been used to study the genetic diversity of foxtail millet species. Similarly, Hirano et al (2011) employed transposons to determine the correlation between geographical area and genetic structure.…”