Genetic variation of isolated Picea balfouriana populations from the southeast of the Qinghai-Tibet Plateau

Lu, Zhuoxuan; Wang, Yuhua; Zhang, Xiaolu; Korpelainen, Helena; Li, Chunyang

doi:10.1051/forest/2009052

Cited by 5 publications

(3 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Habitat fragmentation is expected to have a detrimental impact on the genetic diversity of plant species (O'Connell et al 2006;Isagi et al 2007;Lu et al 2009), but several studies have revealed that population responses to fragmentation are variable (Jump and Penuelas 2006;Kettle et al 2007). In this study, the genetic diversity (H Sne = 0.144) based on ISSR markers was within the general range previously detected for ssp.…”

Section: Discussionsupporting

confidence: 79%

Genetic structure in fragmented populations of Hippophae rhamnoides ssp. sinensis in China investigated by ISSR and cpSSR markers

et al. 2011

Self Cite

View full text Add to dashboard Cite

Hippophae rhamnoides ssp. sinensis is an ecologically and economically important species that has been widely used as a pioneer plant in China. In this study, we employed both nuclear ISSR and maternal cpSSR markers to survey the genetic diversity and structure of populations of ssp. sinensis representing three different landscapes, the northwestern desert and grassland region, the alpine vegetation region of the Qinghai-Tibetan Plateau, and the northeastern humid forest region. In all, 12 natural populations with a scattered distribution in the area were studied. The genetic diversities of populations were found to be uneven, and the total genetic diversity was low on the basis of both types of marker. Mantel tests based on both individual Euclidean distance matrices and population genetic distance (measured by Upt) matrices showed that the two marker systems detected similar trends with respect to genetic distances between populations. The analysis of molecular variance (AMOVA) revealed significant differentiation among populations and among regions for both types of marker. Although the detected pattern of isolationby-distance among all sampled populations confirmed the earlier colonization pathway, the low level of gene flow and the lack of isolation by distance within each region suggested the presence of an additional dispersal barrier. UPGMA dendrograms and PCA plots also revealed clear clustering and significant regional differentiation. Our results indicate that the genetic structure of ssp. sinensis has been affected by habitat fragmentation and restricted population sizes. We propose that the biology of reproduction and ecology have played determinant roles in the development of the regional structure of populations. The genetic information obtained will help to establish conservation strategies and programs for sustainable management of H. rhamnoides ssp. sinensis.

show abstract

Section: Discussionsupporting

confidence: 79%

Genetic structure in fragmented populations of Hippophae rhamnoides ssp. sinensis in China investigated by ISSR and cpSSR markers

et al. 2011

Self Cite

View full text Add to dashboard Cite

show abstract

“…It can also slow down the extinction process caused by adaptation and evolution [ 38 ]. Microsatellite markers are efficient in examining genetic diversity and exploring genetic relationships in plants, and have been widely used to investigate the genetic diversity of Qinghai-Tibetan Plateau species [ 39 , 40 , 41 , 42 , 43 , 44 , 45 ].…”

Section: Discussionmentioning

confidence: 99%

Genetic Structure and Eco-Geographical Differentiation of Lancea tibetica in the Qinghai-Tibetan Plateau

Chi

Zhang

Gao

et al. 2019

Genes

View full text Add to dashboard Cite

The uplift of the Qinghai-Tibetan Plateau (QTP) had a profound impact on the plant speciation rate and genetic diversity. High genetic diversity ensures that species can survive and adapt in the face of geographical and environmental changes. The Tanggula Mountains, located in the central of the QTP, have unique geographical significance. The aim of this study was to investigate the effect of the Tanggula Mountains as a geographical barrier on plant genetic diversity and structure by using Lancea tibetica. A total of 456 individuals from 31 populations were analyzed using eight pairs of microsatellite makers. The total number of alleles was 55 and the number per locus ranged from 3 to 11 with an average of 6.875. The polymorphism information content (PIC) values ranged from 0.2693 to 0.7761 with an average of 0.4378 indicating that the eight microsatellite makers were efficient for distinguishing genotypes. Furthermore, the observed heterozygosity (Ho), the expected heterozygosity (He), and the Shannon information index (I) were 0.5277, 0.4949, and 0.9394, respectively, which indicated a high level of genetic diversity. We detected high genetic differentiation among all sampling sites and restricted gene flow among populations. Bayesian-based cluster analysis (STRUCTURE), principal coordinates analysis (PCoA), and Neighbor-Joining (NJ) cluster analysis based on microsatellite markers grouped the populations into two clusters: the southern branch and the northern branch. The analysis also detected genetic barriers and restricted gene flow between the two groups separated by the Tanggula Mountains. This study indicates that the geographical isolation of the Tanggula Mountains restricted the genetic connection and the distinct niches on the two sides of the mountains increased the intraspecific divergence of the plants.

show abstract

“…Microsatellite, also known as simple sequence repeat (SSR), is widely used in many molecular genetic applications including genotype mapping (ACHERÉ et al, 2004;ECHT et al, 2011;KANG et al, 2010), population structure and genetic diversity analysis (ESPINOZA et al, 2012;PLUESS et al, 2013;LU et al, 2009), and gene flow and germplasm conservation studies (SHIMONO et al, 2011). SSR markers are co-dominant, abundant and evenly distributed in genomes of plants, multi-allelic, highly polymorphic and are easily detectable by simple polymerase chain reaction (PCR) technology (HODGETTS et al, 2001;RAJORA et al, 2001;PEAKALL et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity of Picea likiangensis natural population at different altitudes revealed by EST-SSR markers

et al. 2014

View full text Add to dashboard Cite

Altitude is thought to have greatly influenced current species distribution and their genetic diversity. However, it is unclear how different altitudes have affected the distribution and genetic diversity of Picea likiangensis, a dominant forestry species in the Qinghai-Tibetan Plateau region (QTP). In this study, we investigated the genetic diversity of Picea likiangensis populations which distributed in different altitudes of QTP using EST-SSR markers. The results suggested that this species has high genetic diversity at species level, with 100 % of loci being polymorphic and an average Nei's gene diversity (He) of 0.7186 and Shannon's information index (I) of 1.5415. While the genetic diversity of Picea likiangensis at population level was lower than that at species level, with He and I being 0.6562 and 1.3742, respectively. The variation in genetic diversity of all four studied populations indicated a low-high-low pattern along the elevation gradients. The mid-elevation population (3050 m) was more genetically diverse than both low-elevation (2900 m) and high-elevation populations (3200 m and 3350 m). Nei's genetic diversity (F st = 0.0809) and AMOVA analysis (Phist = 0.1135) indicated that a low level of genetic differentiation among populations. Gene flow among populations was 2.8384, suggesting that high gene flow is a main factor leading to high levels of the genetic diversity among populations.

show abstract

Genetic variation of isolated Picea balfouriana populations from the southeast of the Qinghai-Tibet Plateau

Cited by 5 publications

References 51 publications

Genetic structure in fragmented populations of Hippophae rhamnoides ssp. sinensis in China investigated by ISSR and cpSSR markers

Genetic structure in fragmented populations of Hippophae rhamnoides ssp. sinensis in China investigated by ISSR and cpSSR markers

Genetic Structure and Eco-Geographical Differentiation of Lancea tibetica in the Qinghai-Tibetan Plateau

Genetic diversity of Picea likiangensis natural population at different altitudes revealed by EST-SSR markers

Contact Info

Product

Resources

About