Genetic variation of longtail tuna Thunnus tonggol landed in four fish markets in Indonesia based on mitochondrial DNA

Abstract

Abstract. Astarini IA, Ningsih EY, Simanungkalit D, Ardiana SA, Al Malik MD, Yusmalinda NLA, Sembiring A, Pertiwi NPD, Cahyani NKD, Collins A. 2021. Genetic variation of longtail tuna Thunnus tonggol landed in four fish markets in Indonesia based on mitochondrial DNA. Biodiversitas 22: 1644-1651. Longtail tuna (Thunnus tonggol, Family: Scombridae) is an economically valuable neritic species found in tropical and subtropical waters in the Indo-Pacific region. High catch numbers, which have been decreasing, could negatively impact this tuna’s population level. Little research has been conducted on the longtail tuna population in Indonesia. This study aims to determine the genetic diversity and potential population structure of longtail tuna landed in four fish markets in Indonesia (representing three sampling locations because two markets are relatively close to each other) based on sequences of a region of mitochondrial control region (d-loop). A total of 101 samples, out of 110, were identified and confirmed as T. tonggol species by amplifying and sequencing a fragment of d-loop (amplicons ranging from 482 - 523 bp). Neighbor-joining analysis resulted in a topology with all samples grouped into one clade with an average genetic distance of 0.020. Meanwhile, haplotype diversity (Hd) and nucleotide diversity (?) values of the longtail tuna samples were 0.9939 and 0.0192, respectively. The fixation index (Fst) value was -0.00507, with p> 0.05, which indicates that there is no significant population structure among the longtail tuna collected from four fish markets representing three sampling locations. The results of this analysis can be used as basic data in planning sustainable fisheries management efforts.

The DNA Barcode of cytb on Selais Bungkuk Fish (Hemisilurus heterorhynchus Bleeker) Originating from Riau, Indonesia

“…Beberapa sekuens barkode DNA telah dikembangkan untuk tujuan identifikasi organisme dan studi filogenetik secara molekuler. Barkode DNA yang sudah dikembangkan dan diaplikasikan pada ikan diantaranya sekuens DNA cytochrome b (cytb) (Fernandes et al, 2017;Cutarelli et al 2018;Giusti et al, 2019;Ghouri et al, 2020), cytochrome C oxidase subunit I (COI) (Ude et al, 2020;Lopez-Vaamonde et al, 2021;Batubara et al, 2021), NADH dehydrogenase subunit 5 (ND5) (Ahmed, 2022), serta D-Loop (Ardura, Planes and Garcia-Vazquez, 2013;Sarmiento et al, 2016;Astarini et al, 2021;Elvyra and Afdizan, 2021).…”

Molecular and phylogenetic analysis of Sardinella lemuru Bleeker 1835 at fishing ground Canggu-Bali inferred D-loop mutations of mtDNA

“…Before the DNA extraction step in the laboratory, preserved individual tissue into specific tubes that consists of muscle and fin of caudal and pectoral. This method works under sterile conditions in a concentration of 96% ethanol (C2H5OH) [23]. On the other hand, interviews were conducted with local fishers to confirm the previous artisanal fishery conditions of S. lemuru, the timing of catching and types of fishing gear.…”