Genetic Variation of the First Generation of Rodent Tuber ( Typhonium flagelliforme Lodd.) Mutants Based on RAPD Molecular Markers

Sianipar, Nesti Fronika; Laurent, Danny; Purnamaningsih, Ragapadmi; Darwati, Ireng

doi:10.4308/hjb.22.2.98

Cited by 11 publications

(4 citation statements)

References 18 publications

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“…The previous report of genetic distance obtained was 0.78-0.97 and the dendrogram showed four main clusters in Typhonium flagelliforme mutants (45). The genetic distance ranged from 0.05 to 0.30 based on AFLP marker and 0.13 to 0.44 ranges from RAPD marker observed in 10 mutant lines of cowpea (46). The genetic matrix was calculated to analyse the mutant and control genotype.…”

Section: Discussionmentioning

confidence: 99%

60Co gamma ray induced mutants of cowpea and assessment of genetic variability by SCoT marker

Sankar

Dhanarajan²,

Gurunathan³

et al. 2022

Plant Sci. Today

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Mutagenesis is a well-known technique for introducing new variants into crop plants. In the present study, M2 populations were generated in the cowpea (Vigna unguiculata (L.) Walp.) variety CO7 using gamma irradiation. The M2 progeny were used to investigate the effectiveness of the gamma irradiation doses and examined for the agronomic traits. The genomic variation present in the mutants and their parents was analysed using five SCoT markers. Marker analysis revealed a total of 87 amplicons and among these, 20 amplicons showed polymorphism. The highest numbers of amplicons were observed at SCoT10 (39), while the lowest number of amplicons was produced by SCoT09 (07). The percentage of polymorphism ranged from 18.18% to 28.57%, with an average of 21.12%. Polymorphic information content (PIC) values ranged from 0.197 to 0.345. Analysis of Molecular Variation (AMOVA) showed 12% and 88% between the genotypes and within the genotypes respectively. The constructions of 4 clusters were identified through Unweighted Pair Group Method with Arithmetic Mean (UPGMA) dendrogram tree based on the genetic distance deduced from SCoT marker analysis. Analysis of the genetic relatedness between parent and mutants through Principal Coordinate Analysis (PCoA) revealed two main groups. The present study concludes that the genetic variability induced by gamma irradiation and inherited in the next generations. This research investigation supports that gamma irradiation alters the growth and yield traits, which is helpful for generating the cowpea improvement.

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Section: Discussionmentioning

confidence: 99%

60Co gamma ray induced mutants of cowpea and assessment of genetic variability by SCoT marker

Sankar

Dhanarajan²,

Gurunathan³

et al. 2022

Plant Sci. Today

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“…Primer Pair Matching, primers work in pairs for forward primers and reverse primers because both are used in the same PCR reaction, it must be ensured that the PCR conditions are suitable for both and the temperature difference between the two primers is not more than 5 o C [15]. [11,16,17,18,19]. In conducting this research, it was carried out through the following stages as seen in figure 1:…”

Section: A Polymerase Chain Reactionmentioning

confidence: 99%

Algorithm Model Determination of DNA Primer Design For The Success PCR Process

Siswanto¹,

Sianipar²,

Warnars³

et al. 2022

IJETAE

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Deoxyribonucleic Acid Primer Design is an important factor in the success of the Polymerase Chain Reaction process because it will determine the value of Guanine and Cytosine content and Melting temperature for the genome amplification process in the adequacy of research data. To get a good primer design, a simulation process is needed to get the desired results, namely Guanine and Cytosine content between 50%-60%, Melting temperature 50o -65oC, a minimum length of 70 base pair sequences and the temperature difference between the forward primer and reverse primer less than 5oC. Usually researchers use the Primer3plus or NCBI Primer-BLAST software to get the Deoxyribonucleic Acid Primer Design results. Because there are limitations in the use of the software, the researchers, researchers want to use software that are suitable for current and future research needs. For this reason, a new algorithm model is needed to make software to support the needs of researchers. The method used in the algorithm model is to use Start Codon ATG and Stop Codon TAA, TAG and TGA with sequence lengths of 18, 21 and 24 base pairs. The raw data used is the Deoxyribonucleic Acid of the Typhonium Flagelliforme plant which has potential anti-cancer compounds. The new algorithm model resulted in 22 (twenty two) Deoxyribonucleic Acid Primer Design options, all of which met the data constraints and research requirements.

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“…Ada 37 galur mutan putatif generasi pertama (MV1) di green house yang telah dikarakterisasi secara morfologi dan dianalisis dengan RAPD sehingga diperoleh 15 galur. 15 galur yang dianalisis menunjukkan keragaman dan perubahan genetik dari tanaman kontrol (Sianipar et al, 2015b).…”

Section: Pendahuluanunclassified

Pengembangan Tanaman Keladi Tikus (Typhonium Flagelliforme Lodd.) Asal Indonesia Sebagai Obat Antikanker

Sianipar¹,

Purnamaningsih²,

Rosiana³

2016

ETHOS (Jurnal Penelitian dan Pengabdian)

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Genetic Variation of the First Generation of Rodent Tuber ( Typhonium flagelliforme Lodd.) Mutants Based on RAPD Molecular Markers

Cited by 11 publications

References 18 publications

60Co gamma ray induced mutants of cowpea and assessment of genetic variability by SCoT marker

60Co gamma ray induced mutants of cowpea and assessment of genetic variability by SCoT marker

Algorithm Model Determination of DNA Primer Design For The Success PCR Process

Pengembangan Tanaman Keladi Tikus (Typhonium Flagelliforme Lodd.) Asal Indonesia Sebagai Obat Antikanker

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