Genetic variation of white spot syndrome virus (WSSV) in Pacific white shrimp <i>Litopenaeus vannamei</i>
 (Boone 1931) culture of Thailand

Piamsomboon, Patharapol; Chaweepack, Tidaporn; Han, Jee Eun; Tang, Kathy F.J.; Wongtavatchai, Janenuj

doi:10.1111/are.13456

Cited by 7 publications

(7 citation statements)

References 23 publications

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“…No PCR product was detected for 5 samples. These same results were observed with a third PCR amplification using the primer set TJW75 as described by Piamsomboon et al in 2018 [34] (Table 1). The 35 samples described above gave 702 bp amplicons and no PCR product for the five remaining samples.…”

Section: Vntr Analysis In Orf75supporting

confidence: 82%

“…The first PCR using the primer set ORF14/15-complete [ 29 ] gave 1850 bp amplicon (GenBank Accession MZ327625, Supplementary Table S1 ) for 20 samples out of the 40 samples. To investigate the reason for these negatives results, the two-step PCR as described previously by Piamsomboon et al in 2018 [ 34 ] was applied to the samples. The first step used the primer set TJW14/15 and the second step used the primer set VR14/15-screen ( Table 2 ).…”

Section: Resultsmentioning

confidence: 99%

“…The primer set VR23/24-south [ 26 ] generated a PCR amplicon of 1265 bp (GenBank Accession MZ327626, Supplementary Table S1 ) for 26 of 40 samples. Other PCR amplifications were achieved for 14 out of 40 samples using the primers as described by Dieu et al in 2004, Jiang et al in 2017, and Piamsomboon et al in 2018 [ 26 , 34 , 35 ] ( Table 1 ). None of these PCR amplifications was unable to produce an amplicon for the 14 remaining samples.…”

Section: Resultsmentioning

confidence: 99%

“…The numbers of tandem repeats for VNTRs loci (ORF75, ORF94, ORF125) were analyzed using the Tandem Repeats Finder (TRF) program [ 31 ]. The same sequences of repeat fragments as described previously [ 15 , 16 , 29 , 32 , 33 , 34 ] were used to describe WSSV strains: 45 bp and 102 bp repeat units (RUs) for ORF75, 54 bp RU for ORF94 and 69 bp RU for ORF125. The WSSV genotype was characterized as “N75, N94, N125, X14/15, X23/24” where N is the number of repeat units in a specific ORF and X is the number of base pair deletions in the variable region VR14/15, VR23/24.…”

Section: Methodsmentioning

confidence: 99%

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Genotype Diversity and Spread of White Spot Syndrome Virus (WSSV) in Madagascar (2012–2016)

Onihary

Razanajatovo

Rabetafika

et al. 2021

Viruses

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White Spot Disease (WSD) caused by the White Spot Syndrome Virus (WSSV) is the most devastating viral disease threatening the shrimp culture industry worldwide, including Madagascar. WDS was first reported on the island in 2012; however, little is known about the circulation of the virus and its genetic diversity. Our study aimed at describing the molecular diversity and the spread of WSSV in the populations of Madagascan crustaceans. Farmed and wild shrimps were collected from various locations in Madagascar from 2012 to 2016 and were tested for WSSV. Amplicons from positive specimens targeting five molecular markers (ORF75, ORF94, ORF125, VR14/15 and VR23/24) were sequenced for genotyping characterizations. Four genotypes were found in Madagascar. The type-I genotype was observed in the south-west of Madagascar in April 2012, causing a disastrous epidemic, then spread to the North-West coast. Type-II strains were detected in October 2012 causing an outbreak in another Penaeus monodon farm. In 2014 and 2015, types II and III were observed in shrimp farms. Finally, in 2016, types II and IV were found in wild species including Fenneropenaeus indicus, Metapenaeus monoceros, Marsupenaeus japonicus and Macrobrachium rosenbergii. Considering the economic importance of the shrimp industry for Madagascar, our study highlights the need to maintain WSSV surveillance to quickly take appropriate countermeasures in case of outbreak and to sustain this industry.

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Section: Vntr Analysis In Orf75supporting

confidence: 82%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Genotype Diversity and Spread of White Spot Syndrome Virus (WSSV) in Madagascar (2012–2016)

Onihary

Razanajatovo

Rabetafika

et al. 2021

Viruses

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“…The Sonora isolate has noticeable modi cations on wsv129, wsv178, wsv204 and wsv249 as gain or loss of speci c sequences equivalent to Repeated Motifs (RM) that strongly suggest a role in the infection process. A high variation from 3 to 20 in the number of repetitive sequences in WSV178 has been related to virulence [52,53]. In contrast a lower mean number of repeated sequences was observed in outbreaks, where WSSV genotypes with 5, 6 and 7 of a 54 bp RM were those that predominated in semi-intensive shrimp crops [54].…”

Section: Discussionmentioning

confidence: 99%

In-Vivo Comparative Virulence of Different White Spot Syndrome Virus Isolates In Penaeus Vannamei And Whole Genome Comparison Analysis

Hernández-Montiel¹,

Weidmann

Bekaert

et al. 2021

Preprint

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White Spot Syndrome Virus (WSSV) infects several economically important aquacultural species, causing significant losses to the industry. This virus belongs to the Nimaviridae family, and has a dsDNA genome ranging from 257 to 309 kb (more than 20 isolate genomes fully sequenced and published to date). Multiple routes of infection could be the cause of the high virulence and mortality rates detected in shrimp species. In particular, Penaeus vannamei, differences in isolate virulence have been observed, along with controversy over whether deletions or insertions are associated with gain or loss of virulence.The pathogenicity of three isolates from three locations in Mexico (two from Sinaloa: 'CIAD', and 'Angostura', and one from Sonora: 'Sonora') was evaluated in vivo in white shrimp (P. vannamei) infection assays. Differences were observed in the mortality rate of shrimp among the three isolates, with the Sonora isolate being the most virulent. Subsequently, the complete WSSV genomes were sequenced in depth from the tissues of infected shrimp, and assembled in reference to the genome of isolate CN01 (KT995472), identifying genome sizes for Angostura and Sonora of 289,350 bp and 288,995 bp, respectively. Where three deletion zones were identified compared to CN01 comprising 15 genes, including three envelope proteins VP41A, VP52A and VP41B, one non-structural protein ICP35 and 11 others encoding proteins whose function is currently unknown. In addition, five genes (wsv129, wsv178, wsv204, wsv249 and wsv497) show a modified number of repeat motifs. The main implications and possible effects on viral infection of these modifications are discussed.

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Acute hepatopancreatic necrosis disease

Dong

2022

Aquaculture Pathophysiology

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Genetic variation of white spot syndrome virus (WSSV) in Pacific white shrimp Litopenaeus vannamei (Boone 1931) culture of Thailand

Abstract: White spot syndrome virus (WSSV) is highly pathogenic to penaeid shrimp and has caused significant economic losses in the shrimp farming industry in Thailand. Genotyping analysis was done in 124 WSSV isolates from cultured Pacific white shrimp

Cited by 7 publications

References 23 publications

Genotype Diversity and Spread of White Spot Syndrome Virus (WSSV) in Madagascar (2012–2016)

Genotype Diversity and Spread of White Spot Syndrome Virus (WSSV) in Madagascar (2012–2016)

In-Vivo Comparative Virulence of Different White Spot Syndrome Virus Isolates In Penaeus Vannamei And Whole Genome Comparison Analysis

Acute hepatopancreatic necrosis disease

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