Genetic variation of wild and hatchery populations of the catla Indian major carp (Catla catla Hamilton 1822: Cypriniformes, Cyprinidae) revealed by RAPD markers

Rahman, Shanjida; Khan, Mukhlesur Rahman; Islam, Md. Shahidul; Alam, Samsul

doi:10.1590/s1415-47572009005000013

Cited by 28 publications

(22 citation statements)

References 16 publications

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“…The second clade was further separated into two subgroups, Ado-Ekiti and Esaodo populations in one sub-group, and Akure, Ilesa and Owena populations in the other subgroup. The genetic distance and identity of C. gariepinus from six populations ( (Sultana et al, 2010), C. catla (Rahman et al, 2009) and L. rohita (Islam and Alam, 2004) using RAPD marker analysis. High quality bands with good reproducibility generated Garg et al (2010) who reported 172 to 1677bp in C. batrachus.…”

Section: Resultsmentioning

confidence: 99%

GENETIC VARIABILITY IN CULTURED AND WILD POPULATIONS OF Clarias gariepinus (Osteichthys: Clariidae) USING RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) MARKER

Popoola

Fasakin

Awopetu

2014

CJF

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Three wild populations of Clarias gariepinus from Esaodo (River Osun), Owena (River Owena), and Agbabu (River Oluwa), and three farmed populations, viz: Akure, Ilesa and Ado-Ekiti, in Southwest Nigeria, were analysed for their genetic differences using Random amplified polymorphic DNA (RAPD) analysis. Live specimens comprising 40 individuals (680 ± 3.28 g) from each location were collected and kept in six concrete tanks (2x1x1m). Altogether 435 reproducible bands were obtained from six populations for the nine RAPD primers used. The Analysis of Molecular Variance (AMOVA) indicated that the sampled populations are significantly different from each other, and that 99% of the total variation resided within the population. The percentage of genetic identity (GI) of RAPD-PCR profile among six populations ranged from 74.6% to 83.5%, while Genetic distance of the six populations based on RAPD-PCR profile ranged from 0.180 to 0.293. Estimates of genetic variation in wild and cultured populations of C. gariepinus were made, and total and mean number of segregating fragments were 71 (89.9%), 34.5 and 59 (74.7%), 35.4, respectively. Total gene diversity within wild and cultured populations (Ht) was 0.3419 and 0.3010, respectively. The study established that there is genetic variability in both wild and cultured C. gariepinus. RAPD showed that samples within the wild and cultured populations under study were closer to each other than between the two habitats. With reference to total gene diversity values and total number of segregating fragments, the wild population was considerably more diverse than the cultured population.

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Section: Resultsmentioning

confidence: 99%

GENETIC VARIABILITY IN CULTURED AND WILD POPULATIONS OF Clarias gariepinus (Osteichthys: Clariidae) USING RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) MARKER

Popoola

Fasakin

Awopetu

2014

CJF

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“…This low genetic variation may be due to inbreeding practices usually carried out at fish seed hatcheries. Rahman et al (2009) reported the genetic variation studies among the wild and hatchery stock populations in Bangladesh. High genetic diversity was reported in wild type population and high similarity within the hatchery population.…”

Section: Discussionmentioning

confidence: 99%

Genetic Variability in Labeo rohita, Catla catla and their Hybrid (Labeo rohita ♂ ? Catla catla ♀) Populations Employing Randomly Amplified Polymorphic (RAPD)-Inter Simple Sequence Repeat (ISSR) Assays

Naveed¹

2017

IJAB

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The present study was conducted to estimate genetic diversity of hybrid produced by two major carps (Labeo rohita and Catla catla) using DNA fingerprinting RAPD-ISSRs assay. A total of 65 RAPD-ISSR combinations were studied to check polymorphism among the accessions. 866 loci were amplified with an average of 13.32 loci per primer and 45.26% were found informative. Primers: DDA31-ISSR1 and DDA32-ISSR2 were most informative producing 12 polymorphic bands each. A similarity of 82% and 63% was exhibited by C. catla and their hybrids and L. rohita and their hybrids, respectively. Similarity analysis resulted 92% similarity among L. rohita parents' group and 84% similarity for their hybrids. Cluster analysis divided all accessions into two clusters: A and B; placing L. rohita in-group A, while B cluster was divided into two Sub groups B1 and B2 having C. catla and hybrids, respectively. The low level of genetic diversity, clearly signposted the alarming need of diverse parental populations for fish breeding programs and saving hybrid gene pool losses. It is the first report molecular program for the selection of major carps for breeding of fish in seed hatcheries.

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“…As important characteristics, such markers must be exclusive to the species in question and preferentially monomorphic, that is, found with 100% frequency among the individuals analyzed (Castiglioni and de Campos Bicudo, 2005;Rampelotti et al, 2008;Rahman et al, 2009).…”

Section: Discussionmentioning

confidence: 99%

Genetic polymorphism, molecular characterization and relatedness of Macrobrachium species (Palaemonidae) based on RAPD-PCR

Guerra

Lima²,

Taddei³

et al. 2010

Genet. Mol. Res.

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ABSTRACT. The prawn genus Macrobrachium belongs to the family Palaemonidae. Its species are widely distributed in lakes, reservoirs, floodplains, and rivers in tropical and subtropical regions of South America. Globally, the genus Macrobrachium includes nearly 210 known species, many of which have economic and ecological importance. We analyzed three species of this genus (M. jelskii, M. amazonicum and M. brasiliense) using RAPD-PCR to assess their genetic variability, genetic structure and the phylogenetic relationship between them and to look for molecular markers that enable separation of M. jelskii and M. amazonicum, which are closely related syntopic species. Ten different random decamer primers were used for DNA amplification, yielding 182 fragments. Three of these fragments were monomorphic and exclusive to M. amazonicum or M. jelskii and can be used as specific molecular markers to identify and separate these two species. Similarity indices and a phylogenetic tree showed that M. amazonicum and M. jelskii are closest to each other, while M. brasiliense was the most differentiated species among them; this may be attributed to the different habitat conditions to which these species have been submitted. This information will be useful for further studies on these important crustacean species.

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Genetic variation of wild and hatchery populations of the catla Indian major carp (Catla catla Hamilton 1822: Cypriniformes, Cyprinidae) revealed by RAPD markers

Cited by 28 publications

References 16 publications

GENETIC VARIABILITY IN CULTURED AND WILD POPULATIONS OF Clarias gariepinus (Osteichthys: Clariidae) USING RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) MARKER

GENETIC VARIABILITY IN CULTURED AND WILD POPULATIONS OF Clarias gariepinus (Osteichthys: Clariidae) USING RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) MARKER

Genetic Variability in Labeo rohita, Catla catla and their Hybrid (Labeo rohita ♂ ? Catla catla ♀) Populations Employing Randomly Amplified Polymorphic (RAPD)-Inter Simple Sequence Repeat (ISSR) Assays

Genetic polymorphism, molecular characterization and relatedness of Macrobrachium species (Palaemonidae) based on RAPD-PCR

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