2014
DOI: 10.1111/age.12176
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Genetic variations in the SPP1 promoter affect gene expression and the level of osteopontin secretion into bovine milk

Abstract: Osteopontin (OPN) is now recognized as an important cytokine and extracellular integrin-binding protein at the crossroads of inflammation and homeostasis. In a previous study, we found that OPN gene (SPP1) polymorphisms are associated with milk performance traits and somatic cell score (SCS), a parameter used to estimate the genetic value of udder health in dairy cattle. In this study, we assessed whether the genetic variations had an impact on SPP1 promoter activity, immune response and the level of OPN secre… Show more

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Cited by 26 publications
(30 citation statements)
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“…; Dudemaine et al . ), CSN1S2 , CSN2 , CSN3 and PROLACTIN (Brym et al . ) have been described as candidate genes for milk production or milk composition.…”
Section: Resultsmentioning
confidence: 99%
“…; Dudemaine et al . ), CSN1S2 , CSN2 , CSN3 and PROLACTIN (Brym et al . ) have been described as candidate genes for milk production or milk composition.…”
Section: Resultsmentioning
confidence: 99%
“…The SPP1 gene, encoding for ospeopontin protein, is essential for mammary gland development, milk production, local mammary gland immunity and it has a significant role in the modulation of milk protein gene expression [77, 78]. The FASN gene is directly involved in de novo synthesis of most of the short and medium-chain fatty acids in milk and is essential for the development, functional competence, and maintenance of the lactating mammary gland [61, 79, 80].…”
Section: Discussionmentioning
confidence: 99%
“…Peripheral blood mononuclear cells (PBMCs) were isolated from 700 ml of blood drawn from the jugular vein using two commercial transfusion bags containing citrate-phosphatedextrose-adenine anticoagulant (Animal Blood Resources International, Dixon, CA, USA). The methods used to isolate PBMCs and monocytes by adherence and to culture the MDMs have previously been described (11,37). The identity and purity of CD14+ monocytes were confirmed by flow cytometry using Pacific Blue anti-human CD14 (clone M5E2) antibody as described previously (11).…”
Section: Monocyte Isolation and Mdm Differentiationmentioning
confidence: 99%