Genetic variations of body weight and GCRV resistance in a random mating population of grass carp

Huang, Rong; Sun, Jiaxian; Luo, Qing; He, Libo; Liao, Lanjie; Li, Yongming; Guo, Fuhua; Zhang, Zhu; Wang, Yaping

doi:10.18632/oncotarget.5945

Cited by 22 publications

(20 citation statements)

References 33 publications

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“…Therefore, eight female and eight male wild grass carp individuals from different water systems (Zhujiang river, Yangtse river, Xiangjiang river, Lao river.) that were described in our previous study 24 , were collected. The detection of polymorphism in those wild grass carps confirmed that the natural germplasm resources of wild grass carp were genetically diverse 24 .…”

Section: Resultsmentioning

confidence: 99%

“…that were described in our previous study 24 , were collected. The detection of polymorphism in those wild grass carps confirmed that the natural germplasm resources of wild grass carp were genetically diverse 24 . Further PCR tests were performed in these wild grass carps to confirm that whether the Y-linked sequence is specific to wild male individuals.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Computational identification of Y-linked markers and genes in the grass carp genome by using a pool-and-sequence method

Zhang

Huang

Chen

et al. 2017

Sci Rep

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The molecular analysis of sex in vertebrates is important, as it has the potential to provide vital information for theoretical and applied research alike. Teleost fish are the ancient vertebrates that present a broad sex chromosome system but lack differentiated sex chromosomes in most species. Hence understanding the sex in fish would not only illuminate the sex determination evolution in vertebrates but also shed light on fish farming. In the present study, we used grass carp as a teleost fish model, studied the Y chromosome by using a pool-and-sequence strategy in combination with fragment-ratio method. In total, we identified five Y-linked scaffolds (totaling 347 Kb) and six Y-specific sequences that could be used as sex-specific markers, demonstrating the suitability of NGS-based re-sequencing of pooled DNAs for the identification of sex markers in fish. Moreover, 14 putative Y-linked genes were described for the first time. All the genes, except for un-y1, un-y2, and ubq-y, showed high similarity to their female homologs. RT-PCR revealed that ubq-y was only expressed in the male hypothalamus and pituitary. These findings provided an abundant resource for the Y chromosome of grass carp, and may help elucidate sex chromosome evolution in cyprinid fish.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Computational identification of Y-linked markers and genes in the grass carp genome by using a pool-and-sequence method

Zhang

Huang

Chen

et al. 2017

Sci Rep

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“…And as such, diferent approaches such as SNP, MTLS, AFLP, RAPD, RFLP and QTL analyses have been used for the identiication of disease resistance and susceptibility traits in diferent aquatic organisms. In the case of ish viral diseases, QTL resistance traits have been generated for grass cap reovirus (GCRV) infection in grass carp [179], nervous necrosis virus (NNV) in seabass [180], viral hemorrhagic septicemia (VHS) in turbot [181] and rainbow trout [182], infectious salmon anaemia (ISAV) virus in Atlantic salmon, lymphocytic disease virus in Japanese lounder [183] and infectious pancreatic necrosis virus (IPNV) in Atlantic salmon [184,185]. Among these, the QTL for resistance against IPNV has contributed to signiicantly reducing the IPNV incidence by >80% from 2008 when IPNV resistance ish were introduced in the Norwegian Atlantic salmon industry to 2015 [186].…”

Section: Applications Of Rna-seq and Omics Strategies -From Microorgamentioning

confidence: 99%

Current Advances in Functional Genomics in Aquaculture

Munang’andu¹,

Evensen²

2017

Applications of RNA-Seq and Omics Strategies - From Microorganisms to Human Health

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Gene expression studies in aquaculture have slowly evolved from the traditional reductionist approach of single gene sequencing to high throughput sequencing (HTS) techniques able to sequence entire genomes of living organisms. The upcoming of HTS techniques has led to emergence of metagenomics, nutrigenomics, epigenetics and other omics technologies in aquaculture in the last decade. Metagenomics analyses have accelerated the speed at which emerging pathogens are being discovered, thereby contributing to the design of timely disease control strategies in aquaculture. Using metagenomics, it is easy to identify and monitor microbial communities found in diferent ecosystems. In vaccine production, genomic studies are being used to identify cross neutralizing antigens against variant strains of the same pathogens. In genetics and epigenetics, genomics traits have been identiied that are beginning to gain commercial applications in aquaculture. Nutrigenomics have not only enhanced our understanding of the biological markers for nutrition-related diseases, but they have also enhanced our ability to formulate diets able to maintain a stable immune homeostasis in the gut. Overall, herein, we have shown that functional genomics provide multifaceted applications ranging from monitoring microbial communities in aquatic environments to optimizing production systems in aquaculture.

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“…Grass carp reovirus (GCRV) is an aquareovirus that causes hemorrhagic disease in grass carp ( Ctenopharyngon idellus ) and it is one of the most extensively characterized aquatic reoviruses due to its virulence in vivo and in vitro [4]. GCRV is typically categorized as a type C aquareovirus because it lacks an outer-fiber protein.…”

Section: Introductionmentioning

confidence: 99%

Orthoreovirus outer-fiber proteins are substrates for SUMO-conjugating enzyme Ubc9

Wang

et al. 2016

Oncotarget

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Reoviruses are potential anticancer agents due to their ability to induce cell death in tumor cells. Grass carp reovirus (GCRV) is one of the best characterized models on reovirus pathogenesis in vitro. However, there is little known about how SUMOylation affects reovirus pathogenesis. The SUMO conjugating enzyme 9 (Ubc9) determines the targets of SUMOylation. Here, the protein interactions between reovirus outer fiber proteins, specifically GCRV-104 VP55, and Ubc9 were probed using a yeast two-hybrid system. The N-terminal coiled-coil domain of VP55, containing a single lysine residue, was responsible for the interaction between VP55 and Ubc9 in yeast. In solid phase binding assays, a single amino acid mutation (K87R) prevented Ubc9 from binding to VP55. Overexpression of Ubc9 enhanced GCRV-104 infection efficiency, and knockdown of Ubc9 in CIK cells inhibited viral replication, which suggested that Ubc9 was a proviral factor. Furthermore, Ubc9 was shown to bind outer fiber proteins from type II GCRV, avian reovirus and mammalian reovirus in yeast. To our knowledge, this is the first study to show that Ubc9 binds to reovirus outer-fiber proteins and likely contributes to efficient orthoreovirus replication. These results suggest that SUMOylation modifications could be targeted to improve the therapeutic efficacy of oncolytic reovirus.

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Genetic variations of body weight and GCRV resistance in a random mating population of grass carp

Cited by 22 publications

References 33 publications

Computational identification of Y-linked markers and genes in the grass carp genome by using a pool-and-sequence method

Computational identification of Y-linked markers and genes in the grass carp genome by using a pool-and-sequence method

Current Advances in Functional Genomics in Aquaculture

Orthoreovirus outer-fiber proteins are substrates for SUMO-conjugating enzyme Ubc9

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