Genetic Variations of Fourth Exon Region of Prolactin Gene and Its Associations with Growth Traits in European Sea Bass Dicentrarchus labrax (Moronidae)

Özcan-Gökçek, E.; Işık, Raziye

doi:10.1134/s0032945223030128

Cited by 2 publications

(1 citation statement)

References 39 publications

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“…According to genome-wide association studies (GWAS), milk traits are affected by several candidate genes and these genes could be used in GS. Among the various candidate genes, prolactin gene (PRL) seems to be promising, because it has diverse biological functions such as water and electrolyte balance, growth and development, immunity traits and reproductive functions and it plays crucial roles in mammary gland development, milk secretion and initiation and maintenance of lactation (Khatami et al, 2005;Zhang et al, 2008;César et al, 2012;Ozdemir, 2020;Özcan-Gökçek and Işık, 2023;Samuel et al, 2023).…”

Section: Introductionmentioning

confidence: 99%

Detecting the Molecular Associations of Prolactin Gene Genotypes With Milk Traits in Friesian and Baladi Cows Using RFLP Technique

Khalil,

Iraqi,

Mohamed

et al. 2023

Egyptian Journal of Animal Production

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Four years of experimental work were conducted applying PCR-RFLP to detect the SNP polymorphic associations of the prolactin candidate gene (PRL) with milk traits in cattle. A total of 494 lactations for 180 milking cows of Friesian in Elkarda herd (FK) and Sakha herd (FS) and Baladi cows in Elserw herd (BS) were used. The PCR products were digested using NaeI and SmaI restricted enzymes to investigate the molecular associations of the PRL gene genotypes with milk traits.The highest expected numbers of alleles (NE) in CC, CD and DD genotypes of the PRL gene were recorded in the DD genotype in both Friesian and Baladi herds, while the lowest expected numbers were recorded in CC genotype in all herds. The highest NE in GG, GT and TT genotype was in the TT genotype and the lowest NE was in the GG genotype in all herds. PRL gene was not in Hardy-Weinberg Equilibrium in all herds. The allelic frequency for the D allele was much higher than the frequency of the C allele, while the frequency of the T allele was higher than the frequency of the G allele.The observed and expected values of heterozygosity for the PRL gene were mostly moderate in the three herds studied. The values of polymorphic information content (PIC) were mostly moderate in all herds. The values of fixation index (reduction in heterozygosity due to inbreeding, FIS) were high in the FK herd (0.82), FS herd (0.62) and BS herd (0.94).The molecular associations between PRL gene genotypes and milk traits indicate that the CD genotype of the PRL gene has strong molecular associations with yields of milk, fat and protein relative to CC and DD genotypes. GG genotype was significantly associated with all milk traits relative to GT and TT genotypes.

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Section: Introductionmentioning

confidence: 99%

Detecting the Molecular Associations of Prolactin Gene Genotypes With Milk Traits in Friesian and Baladi Cows Using RFLP Technique

Khalil,

Iraqi,

Mohamed

et al. 2023

Egyptian Journal of Animal Production

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In Silico Protein Investigation and Correlation Analysis of Growth Hormone Gene and Growth Traits in European Sea Bass (Dicentrarchus labrax)

Özcan-Gökçek,

Işık,

Karahan

et al. 2024

Preprint

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The growth hormone (GH) gene plays a regulatory role in postnatal somatic growth, metabolism, and development in vertebrates and fish. The genetic variations in a partial region of the GH gene and its associations with growth traits were studied using DNA sequencing in 200 European sea bass (Dicentrarchus labrax) individuals. We identified 5 haplotypes (HAP1-5) and 10 novel SNPs (g.1557 A > T, g.1611 T > C, g.1663 C > G, g.1799 T > C, g.1824 T > C, g.1912 T > A, and g.2052 G > C) in the GH gene in European sea bass. The genotypes of g.1611 T > C locus of the GH gene were found to be significantly associated with total weight, filet weight, and head length). The association between the GH g.1557A > T genotypes and preanal and abdominal length was statistically significant (p < 0.05). Similarly, the genotypes of g.1857 C > T loci having the synonymous mutation in phenylalanine amino acid were significantly associated with standard length (p < 0.05). HAP4 reported the highest weight and length traits than the other haplotypes (p < 0.05). It is suggested that HAP4 should be used as a potential marker to improve the accuracy of selection in European sea bass in Mediterranean conditions.

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Genetic Variations of Fourth Exon Region of Prolactin Gene and Its Associations with Growth Traits in European Sea Bass Dicentrarchus labrax (Moronidae)

Cited by 2 publications

References 39 publications

Detecting the Molecular Associations of Prolactin Gene Genotypes With Milk Traits in Friesian and Baladi Cows Using RFLP Technique

Detecting the Molecular Associations of Prolactin Gene Genotypes With Milk Traits in Friesian and Baladi Cows Using RFLP Technique

In Silico Protein Investigation and Correlation Analysis of Growth Hormone Gene and Growth Traits in European Sea Bass (Dicentrarchus labrax)

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