2014
DOI: 10.1161/circresaha.114.303475
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Genetically Encoded Ca 2+ Indicators in Cardiac Myocytes

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Cited by 58 publications
(61 citation statements)
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References 159 publications
(154 reference statements)
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“…50,51 As a special consideration to Ca 2+ imaging, the usefulness of super resolution methods is limited by the fact that Ca 2+ diffuses rapidly and small molecule Ca 2+ indicators also have limited on/off kinetics. A promising approach may be to express localized Ca 2+ indicators 5255 in subcellular compartments to further quantify local Ca 2+ levels in relation to sarcomere strain.…”
Section: Discussionmentioning
confidence: 99%
“…50,51 As a special consideration to Ca 2+ imaging, the usefulness of super resolution methods is limited by the fact that Ca 2+ diffuses rapidly and small molecule Ca 2+ indicators also have limited on/off kinetics. A promising approach may be to express localized Ca 2+ indicators 5255 in subcellular compartments to further quantify local Ca 2+ levels in relation to sarcomere strain.…”
Section: Discussionmentioning
confidence: 99%
“…Ca 2ϩ release from the SR is thought to be critical for pacemaker activity in SAMs (5,13,34), and genetically-encoded Ca 2ϩ indicators (GECIs) could provide important new insights into this process. GECIs can be targeted to subcellular microdomains and have yielded much important information about localized Ca 2ϩ signaling in other types of cardiac myocytes (10,11,22,27,36). In a similar manner, tethered FRET-based cAMP sensors could be used to characterize spatio-temporal cAMP signaling associated with different GPCRs and phosphodiesterase isoforms, as has been done in other cardiac myocytes (20,23,30,37,43).…”
Section: Discussionmentioning
confidence: 98%
“…For chemical and genetically encoded Ca 2 + ‐sensitive dyes, the situation is easier, mainly due to the slower kinetics of Ca 2 + fluxes. These probes have already been widely used for high‐throughput screening in both disease modelling and drug development with encouraging results (Kaestner et al , 2014; Huebsch et al , 2015; Lu et al , 2015; Rast et al , 2015; Dempsey et al , 2016; Klimas et al , 2016). Ca 2 + ‐handling also affects cell contractility, which can also be measured, as recent examples with sophisticated optical mapping systems show (Herron et al , 2012; Hayakawa et al , 2014; Maddah et al , 2015).…”
Section: Assays and Readoutsmentioning
confidence: 99%