Genetically Encoded Photoactuators and Photosensors for Characterization and Manipulation of Pluripotent Stem Cells

Pomeroy, Jordan E.; Nguyen, Hung; Hoffman, Brenton D.; Bursac, Nenad

doi:10.7150/thno.20593

Cited by 19 publications

(9 citation statements)

References 194 publications

(224 reference statements)

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“…(f) GECI consist of Ca 2+ -binding CaM, an M13 moiety, and a fluorescent protein. In GCaMP6, binding of Ca 2+ induces the formation of CaM-M13 complex, which protects the fluorescent core and increases its quantum yield 138. (g) GEVI consists of a voltagedependent domain and a fluorescent protein.…”

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confidence: 99%

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Next-generation interfaces for studying neural function

2019

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Monitoring and modulating the diversity of signals used by neurons and glia in a closed-loop fashion is necessary to establish causative links between biochemical processes within the nervous system and observed behaviours. As developments in neural interface hardware strive to keep pace with rapid progress in genetically encoded and synthetic reporters/modulators of neural activity, the integration of multiple functional features becomes a key requirement and a pressing challenge in the field of neural engineering. Electrical, optical, and chemical approaches have been used to manipulate and record neuronal activity in vivo, with a recent focus on technologies that both integrate multiple modes of interaction with neurons into a single device and enable bidirectional communication with neural circuits with enhanced spatiotemporal precision. These technologies are not only facilitating a greater understanding of the brain, spinal cord and peripheral circuits in the context of health and disease, but also informing the development of future closed-loop therapies for neurological, neuro-immune and neuroendocrine conditions.

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confidence: 99%

“…Arclight consist of a voltage-sensing domain fused to super ecliptic pHluorin reporter. Depolarization causes conformation change, which yields a decrease of green fluorescence 138. (h) Single-fiber64 and (i) multi-fiber photometry139 .…”

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confidence: 99%

Next-generation interfaces for studying neural function

2019

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“…[ 193 ] GECIs and GEVIs are fluorescent proteins that offer complementary advantages over electrophysiological approaches by enabling recording from specific subtypes of cells. [ 194–196 ] Fiber photometry uses an implanted optical fiber to both excite and measure fluorescence signals from GECIs or GEVIs, which directly correlate with changes in the neuronal activity of genetically defined subpopulations of neurons. Figure a shows a typical fiber photometry setup.…”

Section: Microsystems For Optical Biointerfacingmentioning

confidence: 99%

Advanced Electrical and Optical Microsystems for Biointerfacing

Obaid

Chen

2020

Advanced Intelligent Systems

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Electrical and optical biointerfaces have contributed considerably to understanding biological systems. Recent advances in biocompatible materials, structure designs, and fabrication techniques have established flexible and minimally invasive electronic/optoelectronic platforms that laminate onto targeted surface regions or implant into precise locations of biosystems to monitor and control various biological processes at cell, tissue, and organ levels. Herein, recent progress in advanced biointegrated electrical and optical platforms is discussed. An overview of materials and device designs to form flexible and even stretchable electrodes is presented. Strategies to reduce tissue damage and foreign‐body response to improve chronic stability are described. State‐of‐the‐art wearable and implantable microsystems with/without wireless capabilities for bioelectrical sensing and stimulation, optical recording and modulation, and multimodal operation are highlighted. In conclusion, a discussion of the remaining obstacles for future research in these areas is provided.

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“…Spatial and temporal gene expression is essential for development and regeneration in multicellular systems1-3. For tissue engineering, spatial and temporal control of gene activation or repression is also needed to recapitulate the heterogeneous complexity and architecture of the tissues intended to model or replace2.…”

Section: Introductionmentioning

confidence: 99%

Optogenetic control of mesenchymal cell fate towards precise bone regeneration

Wang¹,

Huang²,

Ren³

et al. 2019

Theranostics

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Rationale: Spatial-temporal control of cell fate in vivo is of great importance for regenerative medicine. Currently, there remain no practical strategies to tune cell-fate spatial-temporally. Optogenetics is a biological technique that widely used to control cell activity in genetically defined neurons in a spatiotemporal-specific manner by light. In this study, optogenetics was repurposed for precise bone tissue regeneration.Methods: Lhx8 and BMP2 genes, which are considered as the master genes for mesenchymal stem cell proliferation and differentiation respectively, were recombined into a customized optogenetic control system. In the system, Lhx8 was constitutively expressed, while BMP2 together with shLhx8 expression was driven by blue light.Results: As expected, blue light induced BMP2 expression and inactivated Lhx8 expression in cells infected with the optogenetic control system. Optogenetic control of BMP2 and Lhx8 expression inversely regulates MSC fate in vitro. By animal study, we found that blue light could fine-tune the regeneration in vivo. Blue light illumination significantly promotes bone regeneration when the scaffold was loaded with MSCs infected with adeno-Lhx8, GI-Gal4DBD, LOV-VP16, and BMP2-shLhx8.Conclusions: Together, our study revealed that optogenetic control of the master genes for mesenchymal stem cell proliferation and differentiation would be such a candidate strategy for precise regenerative medicine.

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Genetically Encoded Photoactuators and Photosensors for Characterization and Manipulation of Pluripotent Stem Cells

Cited by 19 publications

References 194 publications

Next-generation interfaces for studying neural function

Next-generation interfaces for studying neural function

Advanced Electrical and Optical Microsystems for Biointerfacing

Optogenetic control of mesenchymal cell fate towards precise bone regeneration

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