Genetically Engineered Bacteria Immobilized in Alginate as an Option of Cyanotoxins Removal

Dziga, Dariusz; Sworzen, Magdalena; Władyka, Benedykt; Wasylewski, Marcin

doi:10.7763/ijesd.2013.v4.371

Cited by 9 publications

(11 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The cells of the E. coli BL21- mlrA strain tested previously [ 21 ] have indicated relatively high activity against MCs, three orders of magnitude higher than the natural degrader, the Sphingomonas AMC-3962 strain. Additionally, the same ratio of activity was noticed when cells of both strains were immobilized in alginate [ 13 ]. Based on the compared activity of cells and cell extracts (ratio 1:440, [ 21 ]), it was suspected that only a small portion of MlrA acts directly in the intact cells.…”

Section: Discussionmentioning

confidence: 77%

“…Additionally, the same ratio of activity was noticed when cells of both strains were immobilized in alginate [13]. Based on the compared activity of cells and cell extracts (ratio 1:440, [21]), it was suspected that only a small portion of MlrA acts directly in the intact cells.…”

Section: Discussionmentioning

confidence: 85%

“…Previously [ 13 ], the activity of cells immobilized in alginate beads was shown to be unstable during long-term incubation (three weeks). In a subsequent experiment, the dependence of MC degradation capability on the temperature and long-term stability of the designed system was analyzed ( Figure 4 ).…”

Section: Resultsmentioning

confidence: 99%

“…In our recent report, we indicated that immobilization of MC-degrading bacteria in alginate is a promising option [ 13 ]. Among different natural polysaccharide matrices used in the immobilization technique for microorganisms, entrapment in alginate is one of the most commonly employed [ 25 ].…”

Section: Discussionmentioning

confidence: 99%

“…The previously investigated bioreactors employing natural microorganisms offer relatively low efficiency compared to chemical treatment, and their use in fast purification of large amounts of MC-contaminated water is questionable. Our preliminary experiments [ 13 ] indicated that bacterial cells with significantly increased biodegradation potency are still active toward MC after immobilization in alginate. In the present paper, several novel data have been provided, including: (1) the comparison of open and closed bioreactors; (2) optimizing the efficiency of MC degradation using a different length of column and different initial microcystin LR (MC-LR) concentration; and (3) assays of the long-term efficiency of columns packed with alginate bead-based MC removal at different temperatures.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Bioreactor Study Employing Bacteria with Enhanced Activity toward Cyanobacterial Toxins Microcystins

Dziga

Liszniańska

Władyka

2014

Toxins

Self Cite

View full text Add to dashboard Cite

An important aim of white (grey) biotechnology is bioremediation, where microbes are employed to remove unwanted chemicals. Microcystins (MCs) and other cyanobacterial toxins are not industrial or agricultural pollutants; however, their occurrence as a consequence of human activity and water reservoir eutrophication is regarded as anthropogenic. Microbial degradation of microcystins is suggested as an alternative to chemical and physical methods of their elimination. This paper describes a possible technique of the practical application of the biodegradation process. The idea relies on the utilization of bacteria with a significantly enhanced MC-degradation ability (in comparison with wild strains). The cells of an Escherichia coli laboratory strain expressing microcystinase (MlrA) responsible for the detoxification of MCs were immobilized in alginate beads. The degradation potency of the tested bioreactors was monitored by HPLC detection of linear microcystin LR (MC-LR) as the MlrA degradation product. An open system based on a column filled with alginate-entrapped cells was shown to operate more efficiently than a closed system (alginate beads shaken in a glass container). The maximal degradation rate calculated per one liter of carrier was 219.9 µg h−1 of degraded MC-LR. A comparison of the efficiency of the described system with other biological and chemo-physical proposals suggests that this new idea presents several advantages and is worth investigating in future studies.

show abstract

Section: Discussionmentioning

confidence: 77%

Section: Discussionmentioning

confidence: 85%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations