Genetically engineered filamentous phage for bacterial detection using magnetic resonance imaging

Borg, Raymond E; Ozbakir, Harun F; Xu, Binzhi; Li, Eugene; Fang, Xiwen; Peng, Huan; Chen, Irene; Mukherjee, Arnab

doi:10.1039/d3sd00026e

Cited by 1 publication

(1 citation statement)

References 62 publications

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“…Whole-cell MRI measurements were performed using cell pellets, as described in our previous studies. [20,41,59,60] Briefly, prior to under-going MRI, cells were seeded in in 10 cm tissue culture plates such that they reached confluence within approximately 48 h from the time of seeding. For cells harboring an inducible Aqp1 construct, doxycycline hyclate (0.01-1 μg/mL) was added to the culture media approximately 24 h after seeding.…”

Section: Magnetic Resonance Imagingmentioning

confidence: 99%

A Dual‐Gene Reporter‐Amplifier Architecture for Enhancing the Sensitivity of Molecular MRI by Water Exchange

Huang,

Chen,

Zhu

et al. 2024

ChemBioChem

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The development of genetic reporters for magnetic resonance imaging (MRI) is essential for investigating biological functions in vivo. However, current MRI reporters have low sensitivity, making it challenging to create significant contrast against the tissue background, especially when only a small fraction of cells express the reporter. To overcome this limitation, we developed an approach for amplifying the sensitivity of molecular MRI by combining a chemogenetic contrast mechanism with a biophysical approach to increase water diffusion through the co‐expression of a dual‐gene construct comprising an organic anion transporting polypeptide, Oatp1b3, and a water channel, Aqp1. We first show that the expression of Aqp1 amplifies MRI contrast in cultured cells engineered to express Oatp1b3. We demonstrate that the contrast amplification is caused by Aqp1‐driven increase in water exchange, which provides the gadolinium ions internalized by Oatp1b3‐expressing cells with access to a larger water pool compared with exchange‐limited conditions. We further show that our methodology allows cells to be detected using approximately 10‐fold lower concentrations of gadolinium than that in the Aqp1‐free scenario. Finally, we show that our approach enables the imaging of mixed‐cell cultures containing a low fraction of Oatp1b3labeled cells that are undetectable on the basis of Oatp1b3 expression alone.

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Section: Magnetic Resonance Imagingmentioning

confidence: 99%

A Dual‐Gene Reporter‐Amplifier Architecture for Enhancing the Sensitivity of Molecular MRI by Water Exchange

Huang,

Chen,

Zhu

et al. 2024

ChemBioChem

Self Cite

View full text Add to dashboard Cite

show abstract

Genetically engineered filamentous phage for bacterial detection using magnetic resonance imaging

Abstract: Detecting bacterial cells with high specificity in deep tissues is challenging. Optical probes provide specificity, but are limited by the scattering and absorption of light in biological tissues. Conversely, magnetic...

Cited by 1 publication

References 62 publications

A Dual‐Gene Reporter‐Amplifier Architecture for Enhancing the Sensitivity of Molecular MRI by Water Exchange

A Dual‐Gene Reporter‐Amplifier Architecture for Enhancing the Sensitivity of Molecular MRI by Water Exchange

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