Genetically Engineered <i>Saccharomyces cerevisiae</i> Strain that Can Ultilize Both Xylose and Glucose for Fermentation

Abstract

The primary problem in producing fuel ethanol through microorganism fermentation with lignocellulose is the strain. We constructed a URA3-directed low copy integration-expression plasmid pZMYBX1 and rDNA-directed high copy integration-expression plasmid pZMYX2. Using the lithium acetate transformation method, we co-transformed the linearized plasmid pZMYBX1 (StuI) and pZMYX2 (HpaI) into theS. cerevisiaecells. Ultimately, we obtain three recombinants: HDY-ZMYWBG1, HDY-ZMYWBG2 and HDY-ZMYWBG3. The ethanol yield for HDY-ZMYWBG1 and HDY-ZMYWBG3 are 0.368 g/g and 0.365 g/g, respectively, which are higher than the 0.330 g/g yield for W5. This findings show that the xylose metabolic pathway could be introduced into theS. cerevisiaeto produce an alternative strain for the production of biological ethanol from lignocellulose substrate.

Bioethanol from Lignocellulosic Biomass: Current Findings Determine Research Priorities

“…Upregulation of stress tolerance genes by recombinant DNA technology can be a useful approach to overcome inhibitory situations [ 51 ]. Ge et al [ 52 ] obtained three recombinants: HDY-ZMYWBG1, HDY-ZMYWBG2, and HDY-ZMYWBG3 using the lithium acetate transformation method into the S. cerevisiae cells. The ethanol yield for HDY-ZMYWBG1 and HDY-ZMYWBG3 is 0.368 g/g and 0.365 g/g, respectively.…”