Genetically improved potatoes: protection from damage by Colorado potato beetles

Perlak, Frederick J.; Stone, Terry B.; Muskopf, Yvonne M.; Petersen, Lisa J.; Parker, Gregory B.; McPherson, Sylvia A.; Wyman, J. A.; Love, Stephen L.; Reed, G. L.; Biever, Duane; Fischhoff, David A.

doi:10.1007/bf00014938

Cited by 315 publications

(198 citation statements)

References 31 publications

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“…israelensis used to control larvae of nuisance and vector mosquitoes and blackflies (4)(5)(6)(7). In addition, recombinant DNA technology has been used to transform plants with cry genes yielding insect-resistant transgenic crop plants, including cotton (8), corn (9), potatoes (10), and tomatoes (11). B. thuringiensis-transgenic cotton, corn, and potatoes already are produced commercially in the United States.…”

mentioning

confidence: 99%

CytA enables CryIV endotoxins of Bacillus thuringiensis to overcome high levels of CryIV resistance in the mosquito, Culex quinquefasciatus

Wirth

Georghiou

Federici

1997

Proc. Natl. Acad. Sci. U.S.A.

178

149

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Cry proteins produced by Bacillus thuringiensis are selective biodegradable insecticides used increasingly in bacterial insecticides and transgenic plants as alternatives to synthetic chemical insecticides. However, the potential for development of resistance and cross-resistance in target insect populations to Cry proteins used alone or in combination threatens the more widespread use of this novel pest control technology. Here we show that high levels of resistance to CryIV proteins in larvae of the mosquito, Culex quinquefasciatus, can be suppressed or reduced markedly by combining these proteins with sublethal quantities of CytA, a cytolytic endotoxin of B. thuringiensis. Resistance at the LC 95 level of 127-fold for a combination of three CryIV toxins (CryIVA, B, and D), resulting from 60 generations of continuous selection, was completely suppressed by combining sporulated powders of CytA in a 1:3 ratio with sporulated powders of a CryIVA, CryIVB, and CryIVD strain. Combining the CytA strain with a CryIVA and CryIVB strain also completely suppressed mosquito resistance of 217-fold to the latter toxins at the LC 95 level, whereas combination of CytA with CryIVD reduced resistance in a CryIVD-selected mosquito strain from greater than 1,000-fold to less than 8-fold. The CytA͞CryIV model provides a potential molecular genetic strategy for engineering resistance management for Cry proteins directly into bacterial insecticides and transgenic plants.

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mentioning

confidence: 99%

CytA enables CryIV endotoxins of Bacillus thuringiensis to overcome high levels of CryIV resistance in the mosquito, Culex quinquefasciatus

Wirth

Georghiou

Federici

1997

Proc. Natl. Acad. Sci. U.S.A.

178

149

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“…Three years after rolling out its biotech potatoes, NatureMark varieties comprised four percent of the U.S. crop. By 1991, Monsanto had developed potatoes resistant to both CPB and potato leafroll virus (PLRV) (Perlak et al 1993;Kaniewski and Thomas 2004). In 1998, NatureMark introduced NewLeaf Plus TM , a Russet Burbank variety with resistance to both CPB and PLRV.…”

Section: History Of Biotech Potatomentioning

confidence: 99%

Biotech Potatoes in the 21st Century: 20 Years Since the First Biotech Potato

et al. 2015

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Potato is the world's most important vegetable crop, with nearly 400 million tons produced worldwide every year, lending to stability in food supply and socioeconomic impact. In general, potato is an intensively managed crop, requiring irrigation, fertilization, and frequent pesticide applications in order to obtain the highest yields possible. Important traits are easy to find in wild relatives of potato, but their introduction using traditional breeding can take 15-20 years. This is due to sexual incompatibility between some wild and cultivated species, a desire to remove undesirable wild species traits from adapted germplasm, and difficulty in identifying broadly applicable molecular markers. Fortunately, potato is amenable to propagation via tissue culture and it is relatively easy to introduce new traits using currently available biotech transformation techniques. For these reasons, potato is arguably the crop that can benefit most by modern biotechnology. The benefits of biotech potato, such as limited gene flow to conventionally grown crops and weedy relatives, the opportunity for significant productivity and nutritional quality gains, and reductions in production cost and environmental impact, have the potential to influence the marketability of newly developed varieties. In this review we will discuss current and past efforts to develop biotech potato varieties, traits that could be impacted, and the potential effects that biotech potato could have on the industry.Resumen La papa es el cultivo hortícola más importante en el mundo, con cerca de 400 millones de toneladas producidas a nivel mundial anualmente, acreditando la estabilidad en el suministro de alimentos e impacto socioeconómico. En general, la papa es un cultivo manejado intensivamente, que requiere riego, fertilización y aplicaciones frecuentes de plaguicidas para obtener los más altos rendimientos posibles. Los caracteres importantes son fáciles de encontrar en parientes silvestres de la papa, pero su introducción usando el mejoramiento tradicional puede llevar de 15 a 20 años. Esto es debido a la incompatibilidad sexual entre algunas especies silvestres y cultivadas, el deseo para eliminar características indeseables de las especies silvestres del germoplasma adaptado, y la dificultad en la identificación de marcadores moleculares aplicables ampliamente. Afortunadamente, la papa es receptiva a la propagación por cultivo de tejidos y es relativamente fácil la introducción de nuevos caracteres usando técnicas biotecnológicas de transformación actualmente disponibles. Por estas razones, la papa es probablemente el cultivo que se puede beneficiar mayormente por la biotecnología moderna. Los beneficios de la papa biotecnológica, como el flujo genético limitado a cultivos que se siembran convencionalmente y a los parientes como malezas, la oportunidad para productividad significativa y logros en calidad nutricional, y las reducciones en los costos de producción e impacto ambiental, tienen el potencial para influenciar la comercialización...

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“…Although approved earlier for limited sale, it was not until 1996 that the Monsanto Company (USA) got the approval to commercially market European corn borer (Ostrinia nubilalis) resistant corn (Zea mays L.), Colorado potato beetle (Leptinotarsa decemlineata) resistant potato (Solanum tuberosum L.), cotton bollworm complex (tobacco budworm-Heliothis virescence, bollworm-Helicoverpa zea, and pink bollworm-Pectinophora gossypiell) resistant cotton (Gossypium hirsutum L.), and a non-selective, broad spectrum herbicide glyphosate (N-phosphonomethylglycine) tolerant soybean (Glycine max (L.) Merr.). The genes for all insecticidal proteins, modified cry1Ab in corn [2], modified cry1Ac in cotton [3], and modified cry3Ab in potato [4] derived from commonly found soil bacteria-Bacillus thuringiensis (Bt). Glyphosate tolerant soybean was developed by incorporating a bacterium glyphosate resistant EPSP (5-enolpyruvyl shikimate-3-phosphate) synthase gene [5].…”

Section: Development Of Transgenicmentioning

confidence: 99%

Genetic Engineering of Field, Industrial and Pharmaceutical Crops

Singh¹,

Singh²

2014

AJPS

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Ability to modify plants at the genomic level by advanced molecular technology has enhanced the scope of improvements in plant traits attempted earlier through conventional breeding methods. Techniques such as genetic transformation have opened new vistas whereby functional genes, not commonly present in a particular species can be added from other species. The traits incorporated into the genetically engineered plants in the beginning were confined to those governed by dominant genes, e.g. insecticide resistance and herbicide tolerance but advancements with time now also permit the transfer of complexly inherited traits such as drought and cold tolerance. Transgenic technology is also useful in understanding gene expression and metabolic pathways which can then be used to harness the full genomic potential of the plant. This review presents a narrative on development of transgenics and their use for the improvement of field, industrial and pharmaceuticals crops. In addition, discussions are made on current status on genetically modified crops, hurdles to genetic engineering, overcoming strategies and future scope.

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Genetically improved potatoes: protection from damage by Colorado potato beetles

Cited by 315 publications

References 31 publications

CytA enables CryIV endotoxins of Bacillus thuringiensis to overcome high levels of CryIV resistance in the mosquito, Culex quinquefasciatus

CytA enables CryIV endotoxins of Bacillus thuringiensis to overcome high levels of CryIV resistance in the mosquito, Culex quinquefasciatus

Biotech Potatoes in the 21st Century: 20 Years Since the First Biotech Potato

Genetic Engineering of Field, Industrial and Pharmaceutical Crops

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