Genetically modified whole-cell bioreporters for environmental assessment

Xu, Tingting; Close, Dan; Sayler, Gary S.; Ripp, Steven

doi:10.1016/j.ecolind.2012.01.020

Cited by 81 publications

(56 citation statements)

References 183 publications

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“…More industrial applications are already being developed, such as bacteria to warn a company that its waste flow is too toxic and must be further treated or to report if groundwater is contaminated [52]. Synthetic biologists also hope that living bioreporters can bring applications to the consumer.…”

Section: Communication With Humansmentioning

confidence: 97%

How Synthetic Biology Will Reconsider Natural Bioluminescence and Its Applications

Reeve

Sanderson

Ellis

et al. 2014

Advances in Biochemical Engineering/Biotechnology

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As our understanding of natural biological systems grows, so too does our ability to alter and rebuild them. Synthetic biology is the application of engineering principles to biology in order to design and construct novel biological systems for specific applications. Bioluminescent organisms offer a treasure trove of light-emitting enzymes that may have applications in many areas of bioengineering, from biosensors to lighting. A few select bioluminescent organisms have been well researched and the molecular and genetic basis of their luminescent abilities elucidated, with work underway to understand the basis of luminescence in many others. Synthetic biology will aim to package these light-emitting systems as self-contained biological modules, characterize their properties, and then optimize them for use in other chassis organisms. As this catalog of biological parts grows, synthetic biologists will be able to engineer complex biological systems with the ability to emit light. These may use luminescence for an array of disparate functions, from providing illumination to conveying information or allowing communication between organisms.

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Section: Communication With Humansmentioning

confidence: 97%

How Synthetic Biology Will Reconsider Natural Bioluminescence and Its Applications

Reeve

Sanderson

Ellis

et al. 2014

Advances in Biochemical Engineering/Biotechnology

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“…(2) What is the influence of soil physicochemical parameters on MTE mobility and leachates ecotoxicity? Several studies use specific bacteria strain to sense the presence of metals in soils [25][26][27][28], nevertheless, the development of statistical model to understand the link between chemical concentration of compound and bacteria sensors is still on-going work [23]. So, the originality of this study was to combine the use of new bacterial strains never tested in a context of the remediation of an industrial polluted site and calculation of eco-scores which facilitates the comparisons between different soils.…”

Section: Introductionmentioning

confidence: 99%

Use of ecotoxicity test and ecoscores to improve the management of polluted soils: case of a secondary lead smelter plant

Foucault

Durand

Tack

et al. 2013

Journal of Hazardous Materials

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OATAO is an open access repository that collects the work of Toulouse researchers and makes it freely available over the web where possible. This is an author-deposited version published in : http://oatao.univ-toulouse.fr/ Eprints ID : 11346 a b s t r a c tWith the rise of sustainable development, rehabilitation of brownfield sites located in urban areas has become a major concern. Management of contaminated soils in relation with environmental and sanitary risk concerns is therefore a strong aim needing the development of both useful tools for risk assessment and sustainable remediation techniques. For soils polluted by metals and metalloids (MTE), the criteria for landfilling are currently not based on ecotoxicological tests but on total MTE concentrations and leaching tests. In this study, the ecotoxicity of leachates from MTE polluted soils sampled from an industrial site recycling lead-acid batteries were evaluated by using both modified Escherichia coli strains with luminescence modulated by metals and normalized Daphnia magna and Alivibrio fischeri bioassays. The results were clearly related to the type of microorganisms (crustacean, different strains of bacteria) whose sensitivity varied. Ecotoxicity was also different according to sample location on the site, total concentrations and physico-chemical properties of each soil. For comparison, standard leaching tests were also performed. Potentially phytoavailable fraction of MTE in soils and physico-chemical measures were finally performed in order to highlight the mechanisms. The results demonstrated that the use of a panel of microorganisms is suitable for hazard classification of polluted soils. In addition, calculated eco-scores permit to rank the polluted soils according to their potentially of dangerousness. Influence of soil and MTE characteristics on MTE mobility and ecotoxicity was also highlighted.

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“…Toxicity bioassays sometimes require utilization of genetic engineering with non luminescent organisms [43]. Different expression systems exist.…”

Section: Genetic Transformation With a Luciferase Gene Reporter Systemmentioning

confidence: 99%

Assessing Ecotoxicity in Marine Environment Using Luminescent Microalgae: Where Are We At?

Sanchez-Ferandin¹

2015

AJPS

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Nowadays, microalgae are particularly used to assess the environmental impact of contaminants in aquatic systems. Naturally present in some algal species, bioluminescence is highly used in application fields related to environmental monitoring. Bioluminescent dinoflagellates have played a pivotal role in this domain. When exposed to heavy metals or toxic organic compounds, bioluminescent dinoflagellates have the capacity to decrease light emission. In addition, new molecular tools allow the possibility to produce genetically modified microorganisms which are able to perform luminescence. Combined with the luciferase reporter gene, two main genetic constructions can be employed. Activation of a specific inducible promoter induces the luminescence gene transcription and this signal increases over time. Constitutive promoters result in a high basal expression level of the reporter gene. During exposure to a potential toxic pollutant, the basal expression level will decrease due to the toxic effect. Toxicity bioassays based on engineered luminescent Chlorophyta microalgae are among the most sensitive tests and are an invaluable complement to classical toxicity assays.

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Genetically modified whole-cell bioreporters for environmental assessment

Cited by 81 publications

References 183 publications

How Synthetic Biology Will Reconsider Natural Bioluminescence and Its Applications

How Synthetic Biology Will Reconsider Natural Bioluminescence and Its Applications

Use of ecotoxicity test and ecoscores to improve the management of polluted soils: case of a secondary lead smelter plant

Assessing Ecotoxicity in Marine Environment Using Luminescent Microalgae: Where Are We At?

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