Genetically regulated hepatic transcripts and pathways orchestrate haematological, biochemical and body composition traits

Ponsuksili, Siriluck; Trakooljul, Nares; Hadlich, Frieder; Haack, Fiete; Muráni, Eduard; Wimmers, Klaus

doi:10.1038/srep39614

Cited by 7 publications

(17 citation statements)

References 68 publications

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“…Out of 6321 previously identified transcripts that were correlated with a minimum of one biochemical trait with r = |0.22–0.41| at a significance level of FDR < 1% [ 14 ]; in this study, we obtained those transcripts that were negatively correlated with miRNA expression and were correlated with the same biochemical traits. The transcripts were predicted as targets of miRNAs according to analyses with TargetScan and RNAhybrid.…”

Section: Resultsmentioning

confidence: 99%

“…For haematological traits, the expression levels of miR-652 and miR-204 were found correlated with MCHC and the expression of miR-143 was found correlated with PLT. Eighteen out of 41 mRNA were previously assigned as cis -QTL [ 14 ]. Twelve out of 61 SNPs associated with miR-34a.…”

Section: Resultsmentioning

confidence: 99%

“…Our previous study identified liver transcripts related to cellular and molecular processes and established a comprehensive view of hepatic gene activity linked to metabolic and immune traits in pigs [ 14 ]. The fact that thousands of genes have potential binding sites for miRNAs and single miRNAs can simultaneously target hundreds of genes [ 50 , 51 ] makes the data analysis challenging even with the use of prediction software and criteria.…”

Section: Discussionmentioning

confidence: 99%

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Genetic architecture and regulatory impact on hepatic microRNA expression linked to immune and metabolic traits

et al. 2017

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Regulation of microRNA (miRNA) expression contributes to a wide range of target gene expression and phenotypes. The miRNA expression in the liver, the central metabolic organ, was examined in 209 pigs, and integrated with haematological and clinical biomarkers of metabolic and overall health, mRNA-target expression levels and single-nucleotide polymorphism (SNP) genotypes. The expression levels of 426 miRNA species correlated with plasma haematological or biochemical traits (r² = |0.19–0.45|, false discovery rate < 5%). Pairs of these miRNAs and their predicted target mRNAs showing expressing levels associated with the identical traits were examined to understand how immune and metabolic traits are affected by miRNA-mediated regulatory networks derived by mapping miRNA abundance as an expression quantitative trait. In total, 221 miRNA-expression-QTL correspond to 164 SNPs and 108 miRNAs, including miR-34a, miR-30e, miR-148-3p, miR-204, miR-181-5p, miR-143-5p and let-7 g that also correlate with the biomarkers. Sixty-one SNPs were simultaneously associated with 29 miRNA and 41 mRNA species. The expression levels of 13 out of 29 miRNA were correlated with one of the biochemical or haematological traits. For example, the expression levels of miR-34a were correlated with serum phosphorus and cholesterin levels; miR-204, miR-15a and miR-16b were correlated with triglyceride. For haematological traits, the expression levels of miR-652 and miR-204 were correlated with the mean corpuscular haemoglobin concentration, and the expression of miR-143 was correlated with plateletcrit. Pleiotropic association analyses revealed genetic links between mRNA and miRNA on SSC6 for miR-34a, SSC9 for miR-708 and SSC14 for miR-652. Our analysis of miRNA and mRNA transcript profiles, their correlation with clinically important plasma parameters of hepatic functions as well as information on their genetic regulation provide novel regulatory networks and potential new biomarkers for immune and metabolic traits.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Genetic architecture and regulatory impact on hepatic microRNA expression linked to immune and metabolic traits

et al. 2017

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“…Plasma UN concentration reflects the balance status of amino acids and is often used as an indicator of kidney and liver function. Low level of blood UN indicates a good balance of amino acids and suggests relatively low urea synthesis and hydration in the liver, and relatively high dietary protein efficiency (Ponsuksili et al, ). In the present study, we found that the plasma concentration of Alb increased linearly, while activity of CHE decreased with increasing amounts of mulberry.…”

Section: Discussionmentioning

confidence: 99%

Dietary mulberry leaf powder affects growth performance, carcass traits and meat quality in finishing pigs

Liu

Peng

et al. 2019

Animal Physiology Nutrition

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This study was conducted to evaluate the effect of mulberry leaves as an alternative source of protein on growth performance, carcass traits and meat quality in finishing pigs. A total of 180 Xiangcun Black pigs were randomly assigned to five treatment groups with six pens of six pigs per pen. The pigs were provided with a basal diet or a diet contained 3%, 6%, 9% or 12% of mulberry leaf powder during a 50‐day experiment period. The results showed that dietary mulberry leaf powder had no negative effect on growth performance in Xiangcun Black pigs, except in the 12% mulberry group, where final body weight and average daily gain decreased (p < .05) and feed to gain ratio of the pigs increased (p < .05). Dietary mulberry inclusion decreased (quadratic, p < .05) the back fat thickness, fibre mean cross‐sectional area (CSA) in the longissimus dorsi (LD) muscle and mRNA expression levels of myosin heavy chain (MyHC) IIb in LD and biceps femoris (BF) muscles, while increased (linear or quadratic, p < .05) the plasma concentration of albumin, levels of crude protein (CP), inosine monophosphate (IMP) and several amino acids in muscle tissues. When compared with the other groups, the 9% mulberry diet increased (p < .05) loin‐eye area and contents of CP and IMP in muscles, while decreased (p < .05) plasma activity of cholinesterase and concentrations of uric acid and urea. The 6% mulberry diet had the lowest fibre mean CSA and shear force and increased total fibre number of the LD muscle, when compared with the other groups. These results suggest that including mulberry in the diet at <12% is an effective feed crop to improve meat quality and the chemical composition of muscle without negatively affecting growth performance.

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“…In chicken and quail populations, estimations have shown a considerable heritability of approximately 10% [62,63,66]. However, holistic and genome-wide association studies (GWAS) for P-relevant traits are still missing for pig [67,68]. It can be assumed that the heritabilities are similar to quails and chicken, allowing the underlying genetic variation to be elucidated and subsequently used in breeding programs.…”

Section: Animal Geneticsmentioning

confidence: 99%

Bridging Gaps in the Agricultural Phosphorus Cycle from an Animal Husbandry Perspective—The Case of Pigs and Poultry

et al. 2018

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Since phosphorus (P) is an essential element for life, its usage and application across agricultural production systems requires great attention. Monogastric species such as pigs and poultry can significantly contribute to global food security but these animals remain highly dependent on the supply of mineral inorganic P in their feeds. Pig and poultry, which represent 70% of the global meat production, are also major P excretors and thus represent important sources of environmental P inputs. Balancing the P cycle within farming systems is crucial to achieve P sustainable and resilient livestock production. Therefore, the interconnection of animal feed, livestock farming, manure, and soil/aquatic ecosystems requires multidisciplinary approaches to improve P management. With regard to a sustainable agricultural P cycle, this study addresses aspects of feeding strategies and animal physiology (e.g., phase feeding, P conditioning, liquid feeding, phytase supplementation, genetics), soil agroecosystems (e.g., P cycling, P losses, P gains), reuse and recycling (e.g., manure, slaughter waste), measures of farmers' economic performance (e.g., bio-economic models), and P governance/policy instruments (e.g., P quota, P tax). To reconcile the economic and ecological sustainability of animal husbandry, the strategic objective of future research will be to provide solutions for a sufficient supply of high-quality animal products from resource-efficient and economically competitive agro-systems which are valued by society and preserve soil and aquatic ecosystems.

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Genetically regulated hepatic transcripts and pathways orchestrate haematological, biochemical and body composition traits

Cited by 7 publications

References 68 publications

Genetic architecture and regulatory impact on hepatic microRNA expression linked to immune and metabolic traits

Genetic architecture and regulatory impact on hepatic microRNA expression linked to immune and metabolic traits

Dietary mulberry leaf powder affects growth performance, carcass traits and meat quality in finishing pigs

Bridging Gaps in the Agricultural Phosphorus Cycle from an Animal Husbandry Perspective—The Case of Pigs and Poultry

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