Genetically targeted chemical assembly of functional materials in living cells, tissues, and animals

Liu, Jia; Kim, Yoon Seok; Richardson, Charlotte; Tom, Ariane; Ramakrishnan, Charu; Birey, Fikri; Katsumata, Tōru; Chen, Shu‐Cheng; Wang, Cheng; Wang, Xiao; Joubert, Lydia‐Marie; Jiang, Yuanwen; Wang, Shuangfeng; Fenno, Lief E.; Tok, Jeffrey B.‐H.; Paşca, Sergiu P.; Shen, Kang; Bao, Zhenan; Deisseroth, Karl

doi:10.1126/science.aay4866

Cited by 160 publications

(169 citation statements)

References 32 publications

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“…1b and Supplementary Data 1 ). Furthermore, consistent with recent report for synthesis of aniline-based polymers catalyzed by APEX2 in living cells 21 , 22 , we found BN2 could efficiently form red-colored polymers (Supplementary Fig. 1c ).…”

Section: Resultssupporting

confidence: 92%

Spatiotemporal profiling of cytosolic signaling complexes in living cells by selective proximity proteomics

Yuan

et al. 2021

Nat Commun

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Signaling complexes are often organized in a spatiotemporal manner and on a minute timescale. Proximity labeling based on engineered ascorbate peroxidase APEX2 pioneered in situ capture of spatiotemporal membrane protein complexes in living cells, but its application to cytosolic proteins remains limited due to the high labeling background. Here, we develop proximity labeling probes with increased labeling selectivity. These probes, in combination with label-free quantitative proteomics, allow exploring cytosolic protein assemblies such as phosphotyrosine-mediated protein complexes formed in response to minute-scale EGF stimulation. As proof-of-concept, we systematically profile the spatiotemporal interactome of the EGFR signaling component STS1. For STS1 core complexes, our proximity proteomics approach shows comparable performance to affinity purification-mass spectrometry-based temporal interactome profiling, while also capturing additional—especially endosomally-located—protein complexes. In summary, we provide a generic approach for exploring the interactome of mobile cytosolic proteins in living cells at a temporal resolution of minutes.

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Section: Resultssupporting

confidence: 92%

Spatiotemporal profiling of cytosolic signaling complexes in living cells by selective proximity proteomics

Yuan

et al. 2021

Nat Commun

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“…In situ polymerization on cell surface 11 and enzymatic reaction in the cells 13,66,67 or tissues 68 Neuron regulation 11 and cancer therapy 13,[65][66][67][68] Ultrasound Piezoelectric materials 3D cyclic mechanical stimulation, 76 electrical stimulation, 77,78 ultrasound stimulation for polymer, 79 nanoparticle-laden cells, 80 and microswimmers 81 Stem cell differentiation, 76,77,78 signal transduction, 79,80 and cell delivery and differentation 81 controlled in 2D arrays by an external magnetic field on the surface of substrate materials. These 2D arrays of the MNPs enable reversible cell adhesion on the substrate surface.…”

Section: Organometallic Complexationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…In situ self-assembly can be remotely induced via an injection of cytocompatible ligands, precursors, peptides, and ions into the targeted sites. [11][12][13] Multifarious nanoengineered in situ self-assembly can be extracellularly and intracellularly induced in the presence of living cells by exploiting unique and cytocompatible biochemical interactions. Piezoelectricity can be actively induced by externally exerting mechanical stimulation, electrical potential, and ultrasound, which is particularly beneficial in activating electrically excitable tissues, such as the nervous, bone, and muscle tissues.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Remote active control of nanoengineered materials for dynamic nanobiomedical engineering

Kim

Choi

Shin

et al. 2020

VIEW

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Cells dynamically interact with native nanostructured extracellular matrix at a molecular level in vivo. Developing remotely and actively controllable nanoengineered biomaterials can manipulate and unravel complex cell-material interactions that dynamically occur in the nanoscale in vivo. In this review, we discuss emerging advances in a myriad of recent nanoengineering technologies to design remotely manipulable materials that enable dynamic nanobiomedical engineering at the molecular level. In particular, we focus on remote active stimuli, such as magnetic fields, light, in situ self-assembly, and ultrasound, to manipulate dynamic cell-material interactions in both in vitro and in vivo settings. Remote active control can be particularly appealing with targeting capability for particular locations at any prescribed time points with a degree of reversibility. The unique remote controllability enables the regulation of cellular signaling, adhesion, differentiation, and polarization; cell, drug, and gene delivery; and in situ self-assembly. These materials allow the remote control in regenerative medicine, immunotherapy, cancer therapy, and biocatalysis as well as mechanistic studies on dynamic nanoscale cell-material interactions. We also highlight current challenges in the remote active control, such as reproducibility, tissue-penetrative capability, noninvasive surgery, spatial localization, and temporal variation. Albeit remotely and actively controllable nanoengineered biomaterials are in the nascent stage of development, they can evolve into multiresponsive, reversible, and cost-effective three-dimensional systems with safe and convenient long-term control at the cell, tissue, and organ level toward clinical patient-tailorable on-demand therapy. K E Y W O R D S dynamic nanobiomedical engineering, magnetic control, nanoengineered biomaterial, photonic control, remote active control, self-assembly-based control This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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“…Enlightened by a microbial medicine factory 9,10 , a concept has emerged in which a biological body can act as a bioreactor for in situ production of functional nanoagents via chemical reaction or physical assembly of injected precursors. The speci c species and microenvironment within the biological body can act as catalysts, stimuli or reactants 11,12 . This biosynthesis technology holds great potential for overcoming the systemic toxicity and low delivery e ciency that are associated with current drug delivery systems 13 .…”

Section: Introductionmentioning

confidence: 99%

In Situ Silver-based Electrochemical Bioreactor In Vivo

Huang¹,

Zhong²,

Deng³

et al. 2020

Preprint

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In this study we show for the first time that a reduced graphene oxide (rGO) carrier has a 15-fold higher catalysis rate than graphene oxide (GO) in Ag+ reduction. Based on this, we constructed a tumor microenvironment-enabled in situ silver-based electrochemical oncolytic bioreactor (SEOB) which unlocked an Ag+ prodrug to generate silver nanoparticles and inhibited the growth of various tumors. In this bioreactor system, intratumoral H2O2 acted as the reductant and the rGO carrier acted as the catalyst. Chelation of aptamers to this prodrug increased the production of silver nanoparticles by tumor cells, especially in the presence of Vitamin C, which broke down in tumor cells to supply massive amounts of H2O2. Consequently, highly efficient silver nanoparticle-induced apoptosis was observed in HepG2 and A549 cells in vitro and in HepG2- and A549-derived tumors in vivo. The apoptosis was associated with ROS-induced changes in mitochondrial membrane potential and DNA damage. The specific aptamer targeting and intratumoral silver nanoparticle production guaranteed excellent biosafety, with no damage to normal cells, because the Ag+ prodrug was specifically unlocked in tumors. More significantly, there was no evident tissue damage in monkeys, which greatly increases the clinical translation potential of the SEOB system.

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Genetically targeted chemical assembly of functional materials in living cells, tissues, and animals

Cited by 160 publications

References 32 publications

Spatiotemporal profiling of cytosolic signaling complexes in living cells by selective proximity proteomics

Spatiotemporal profiling of cytosolic signaling complexes in living cells by selective proximity proteomics

Remote active control of nanoengineered materials for dynamic nanobiomedical engineering

In Situ Silver-based Electrochemical Bioreactor In Vivo

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