Genetically transformed roots: from plant disease to biotechnological resource

Georgiev, Milen I.; Agostini, Elizabeth; Ludwig‐Müller, Jutta; Xu, Jianfeng

doi:10.1016/j.tibtech.2012.07.001

Cited by 185 publications

(119 citation statements)

References 72 publications

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“…The molecular basis of hairy root induction is a transfer DNA (T-DNA) from root inducing (Ri) plasmid of A. rhizogenes. T-DNA integrated into the genome has rol gene loci (rol A, B, C) which have a major role in promoting phytochemical production in genetically transformed roots (Georgiev et al 2012).…”

Section: Introductionmentioning

confidence: 99%

In vitro regeneration and Agrobacterium mediated genetic transformation of Artemisia aucheri Boiss

Sharafi

Sohi

Mirzaee

et al. 2014

Physiol Mol Biol Plants

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In the present study, we developed an efficient protocol for in vitro plant regeneration and genetically transformed root induction in medicinal plant Artemisia aucheri Boiss. Leaf explants were cultivated in MS medium supplemented by combination of plant growth regulators including α-naphthalene-acetic acid, 6-benzyl-aminopurine, indole-3-acetic acid and 2, 4-dichlorophenoxyaceticacid. The highest frequency of shoot organogenesis occurred on MS medium supplemented with 0.05 mg/l NAA plus 2 mg/l BA (96.3 %) and MS medium supplemented with 0.5 mg/l IAA plus 2 mg/l BA (88.3 %). Root induction was obtained on MS medium supplemented with 0.5 mg/l IBA. This is a simple, reliable, rapid and high efficient regeneration system for A. aucheri Boiss in short period via adventitious shoot induction approach. Also, an efficient genetically transformed root induction for A. aucheri was developed through Agrobacterium rhizogenes-mediated transformation by four bacterial strains, A4, ATCC15834, MSU440, and A13 (MAFF-02-10266). The maximum frequency of hairy root induction was obtained using MSU440 (93 %) and ATCC15834 (89 %) bacterial strains. Hairy root lines were confirmed by PCR using the rolB gene specific primers and Southern blot analysis.

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Section: Introductionmentioning

confidence: 99%

In vitro regeneration and Agrobacterium mediated genetic transformation of Artemisia aucheri Boiss

Sharafi

Sohi

Mirzaee

et al. 2014

Physiol Mol Biol Plants

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“…However, Agrobacterium sp. was usually described as aerobic microorganisms that could inhabit plant tissues causing tumors [12]. Therefore, most of strains in the genus are plant pathogens, implying the limitation of their application.…”

Section: Discussionmentioning

confidence: 99%

Heterotrophic Nitrification and Aerobic Denitrification by Four Novel Isolated Bacteria

Li¹,

Wu²,

Shen³

et al. 2015

Pol. J. Environ. Stud.

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“…Now hairy root cultures are considered to be green factories for high-value molecules (Georgiev et al 2012). Our previous study reported that hairy root cultures of R. verticillata possessed higher level of ajmalicine than other organs, such as roots, stems, old leaves, young leaves and barks (Liu et al 2012).…”

Section: Bioinformatics Analysis Of Rvmctmentioning

confidence: 99%

Molecular cloning and characterization of the gene encoding 2-C-methyl-D-erythritol 4-phosphate cytidyltransferase from hairy roots of Rauvolfia verticillata

Lan

2012

Biologia

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2-C-methyl-D-erythritol 4-phosphate cytidyltransferase (MCT) catalyzes the third reaction in the plastidial non-mevalonate pathway, which provides the precursors for ajmalicine. A full-length cDNA encoding MCT (RvMCT) was identified from hairy roots of Rauvolfia verticillata. The full-length 1,499-bp cDNA of RvMCT had a 945-bp coding sequence that encoded a 314-amino-acid protein with an N-terminal chloroplast transit peptide of 67 amino acid residues. RvMCT exhibited homology with other plant MCTs at the levels of sequence and structure. The phylogenetic analysis revealed the plant MCTs could be divided into three separated clusters including gymnosperms, monocotyledons and dicotyledons. Gene expression of ajmalicine metabolism (DXR, MCT, MECS, HDS, HDR, STR and SGD) in hairy roots, roots, stems, old leaves, young leaves and barks was analyzed by quantitative PCR. All the seven genes had higher expression levels in hairy roots than in other plant organs. This suggested hairy roots of R. verticillata possessed more active alkaloid metabolism than other organs and it was the reason that hairy roots produced higher levels of ajmalicine. Furthermore, the expression of DXR, MECS, HDS, HDR, STR and SGD genes was not detected in stems (only MCT detected in stems), so it could be presumed that stem acted as a transporter tissue of ajmalicine. Finally, the colour complementation assay indicated that the function of RvMCT was the same as Arabidopsis MCT. Molecular cloning, characterization and functional identification of RvMCT will be helpful to understand more about the role of MCT involved in ajmalicine biosynthesis at the molecular level.

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Genetically transformed roots: from plant disease to biotechnological resource

Cited by 185 publications

References 72 publications

In vitro regeneration and Agrobacterium mediated genetic transformation of Artemisia aucheri Boiss

In vitro regeneration and Agrobacterium mediated genetic transformation of Artemisia aucheri Boiss

Heterotrophic Nitrification and Aerobic Denitrification by Four Novel Isolated Bacteria

Molecular cloning and characterization of the gene encoding 2-C-methyl-D-erythritol 4-phosphate cytidyltransferase from hairy roots of Rauvolfia verticillata

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